Preliminary report on cross-species microsatellite amplification for bumblebee biodiversity and conservation studies

The Iberian Peninsula holds a high diversity of bumblebees but there is a general lack of information about their biodiversity in this area. To overcome this and facilitate conservation studies, we present two novel multiplex assays for the amplification of six and five microsatellite loci respectiv...

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Detalles Bibliográficos
Autores: Cejas Acuña, D., Ornosa Gallego, Concepción, Muñoz Gabaldón, I., Rúa Tarín, P. de la
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/13877
Acceso en línea:https://hdl.handle.net/20.500.14352/13877
Access Level:acceso abierto
Palabra clave:595.799(460)
Conservation genetics
Apidae
Bombus
Molecular markers
Iberian Peninsula
Genética de la conservación
Marcadores moleculares
Península Ibérica
Insectos
2413 Biología de Insectos (Entomología)
Descripción
Sumario:The Iberian Peninsula holds a high diversity of bumblebees but there is a general lack of information about their biodiversity in this area. To overcome this and facilitate conservation studies, we present two novel multiplex assays for the amplification of six and five microsatellite loci respectively. Both assays successfully amplified for most of the studied species in the Iberian populations. Sibling workers and population genetic parameters were analysed in the managed species B. terrestris and in the wild species B. monticola and B. mesomelas, demonstrating the capability of these multiplex assays for biodiversity studies of both managed and wild bumblebee species.