Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins

Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs)...

Descripción completa

Detalles Bibliográficos
Autores: Costa-Hurtado, Mar, Garcia-Rodriguez, Laura, Lopez-Serrano, Sergi, Aragon, Virginia
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/549
Acceso en línea:http://hdl.handle.net/20.500.12327/549
https://doi.org/10.1186/s13567-019-0687-2
Access Level:acceso abierto
Palabra clave:619
id ES_8aafa56006fcedd1c1cfce0db2d4a777
oai_identifier_str oai:recercat.cat:20.500.12327/549
network_acronym_str ES
network_name_str España
repository_id_str
spelling Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteinsCosta-Hurtado, MarGarcia-Rodriguez, LauraLopez-Serrano, SergiAragon, Virginia619Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs). VtaA2 is up-regulated during infection and is only found in virulent strains. In order to determine its biological function, the vtaA2 gene was cloned with its native promotor region in pACYC184, and the transformed Escherichia coli was used to perform functional in vitro assays. VtaA2 was found to have a role in attachment to plastic, mucin, BSA, fibronectin and collagen. As other VtaAs from H. parasuis, the passenger domain of VtaA2 contains collagen domains. In order to examine the contribution of the collagen repeats to VtaA2 function, a recombinant vtaA2 without the central collagen domains was obtained and named vtaA2OL. VtaA2OL showed similar capacity than VtaA2 to adhere to plastic, mucin, BSA, fibronectin and plasma but a reduced capacity to adhere to collagen, suggesting that the collagen domains of VtaA2 are involved in collagen attachment. No function in cell adhesion and invasion to epithelial alveolar cell line A549 or unspecific binding to primary alveolar macrophages was found. Likewise VtaA2 had no role in serum or phagocytosis resistance. We propose that VtaA2 mediates adherence to the host by binding to the mucin, found in the upper respiratory tract mucus, and to the extracellular matrix proteins, present in the connective tissue of systemic sites, such as the serosa.info:eu-repo/semantics/publishedVersionBMCProducció AnimalSanitat Animal2019info:eu-repo/semantics/article9http://hdl.handle.net/20.500.12327/549https://doi.org/10.1186/s13567-019-0687-2reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVeterinary ResearchMINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2016-77361-R/ES/Papel de la microbiota respiratoria en la prevención de las enfermedades causadas por los colonizadores precoces del tracto respiratorio superior del cerdo/MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/IJCI-2016-30204/ES/ /Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/5492026-05-29T05:05:01Z
dc.title.none.fl_str_mv Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
title Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
spellingShingle Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
Costa-Hurtado, Mar
619
title_short Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
title_full Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
title_fullStr Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
title_full_unstemmed Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
title_sort Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins
dc.creator.none.fl_str_mv Costa-Hurtado, Mar
Garcia-Rodriguez, Laura
Lopez-Serrano, Sergi
Aragon, Virginia
author Costa-Hurtado, Mar
author_facet Costa-Hurtado, Mar
Garcia-Rodriguez, Laura
Lopez-Serrano, Sergi
Aragon, Virginia
author_role author
author2 Garcia-Rodriguez, Laura
Lopez-Serrano, Sergi
Aragon, Virginia
author2_role author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs). VtaA2 is up-regulated during infection and is only found in virulent strains. In order to determine its biological function, the vtaA2 gene was cloned with its native promotor region in pACYC184, and the transformed Escherichia coli was used to perform functional in vitro assays. VtaA2 was found to have a role in attachment to plastic, mucin, BSA, fibronectin and collagen. As other VtaAs from H. parasuis, the passenger domain of VtaA2 contains collagen domains. In order to examine the contribution of the collagen repeats to VtaA2 function, a recombinant vtaA2 without the central collagen domains was obtained and named vtaA2OL. VtaA2OL showed similar capacity than VtaA2 to adhere to plastic, mucin, BSA, fibronectin and plasma but a reduced capacity to adhere to collagen, suggesting that the collagen domains of VtaA2 are involved in collagen attachment. No function in cell adhesion and invasion to epithelial alveolar cell line A549 or unspecific binding to primary alveolar macrophages was found. Likewise VtaA2 had no role in serum or phagocytosis resistance. We propose that VtaA2 mediates adherence to the host by binding to the mucin, found in the upper respiratory tract mucus, and to the extracellular matrix proteins, present in the connective tissue of systemic sites, such as the serosa.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/549
https://doi.org/10.1186/s13567-019-0687-2
url http://hdl.handle.net/20.500.12327/549
https://doi.org/10.1186/s13567-019-0687-2
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Veterinary Research
MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2016-77361-R/ES/Papel de la microbiota respiratoria en la prevención de las enfermedades causadas por los colonizadores precoces del tracto respiratorio superior del cerdo/
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/IJCI-2016-30204/ES/ /
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412735652986880
score 15,811543

Similares en LA Referencia