A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics

19 p.-1 fig.

Detalles Bibliográficos
Autores: Berbel, Dàmaris, Cámara, Jordi, García, Ernesto, Tubau, Fe, Guérin, François, Giard, Jean-Christophe, Domínguez, M. Ángeles, Cattoir, Vincent, Ardanuy, Carmen
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::33023e25673c79ff6827f47f7ad16228
Acceso en línea:http://hdl.handle.net/10261/192378
Access Level:acceso abierto
Palabra clave:GAS
L phenotype
LS(A)P phenotype
Streptococcus pyogenes
lnu(B)
lsa(E)
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network_name_str España
repository_id_str
spelling A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibioticsBerbel, DàmarisCámara, JordiGarcía, ErnestoTubau, FeGuérin, FrançoisGiard, Jean-ChristopheDomínguez, M. ÁngelesCattoir, VincentArdanuy, CarmenGASL phenotypeLS(A)P phenotypeStreptococcus pyogeneslnu(B)lsa(E)19 p.-1 fig.A lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes [group A streptococcus (GAS)]. The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics. Antimicrobial susceptibility was tested using the microdilution broth method and the resistance phenotype was tested by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). Whole-genome sequencing revealed that the strain was ST10 and emm93, and had five resistance genes [lnu(B), ant(6)-Ia, aph(3')-III, tet(M) and dfrG]. The tet(M) gene was located in a Tn916-like transposon. The lsa(E)-lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Ia-sat4-aph(3')III] and lsa(E)-lnu(B) genes. This structure was not transferred by conjugation. This study identified a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS.This study was supported by grants from Fondo de Investigaciones Sanitarias de la Seguridad Social (INT 15/0186; INT16/0117 and PI18/00339) and Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES CB06/06/0037), an initiative of the Instituto de Salud Carlos III, Madrid, Spain. Financial support was also provided by the European Regional Development Fund and by CERCA Programme.Peer reviewedElsevierInstituto de Salud Carlos IIIEuropean CommissionGarcía, Ernesto [0000-0002-1741-5486]Tubau, Fe [0000-0002-6416-6451]Giard, Jean-Christophe [0000-0001-8588-2732]Cattoir, Vincent [0000-0003-4026-9912]Ardanuy, Carmen [0000-0003-0225-607X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/192378reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.ijantimicag.2019.08.019Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::33023e25673c79ff6827f47f7ad162282026-05-22T06:33:51Z
dc.title.none.fl_str_mv A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
title A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
spellingShingle A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
Berbel, Dàmaris
GAS
L phenotype
LS(A)P phenotype
Streptococcus pyogenes
lnu(B)
lsa(E)
title_short A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
title_full A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
title_fullStr A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
title_full_unstemmed A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
title_sort A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics
dc.creator.none.fl_str_mv Berbel, Dàmaris
Cámara, Jordi
García, Ernesto
Tubau, Fe
Guérin, François
Giard, Jean-Christophe
Domínguez, M. Ángeles
Cattoir, Vincent
Ardanuy, Carmen
author Berbel, Dàmaris
author_facet Berbel, Dàmaris
Cámara, Jordi
García, Ernesto
Tubau, Fe
Guérin, François
Giard, Jean-Christophe
Domínguez, M. Ángeles
Cattoir, Vincent
Ardanuy, Carmen
author_role author
author2 Cámara, Jordi
García, Ernesto
Tubau, Fe
Guérin, François
Giard, Jean-Christophe
Domínguez, M. Ángeles
Cattoir, Vincent
Ardanuy, Carmen
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
García, Ernesto [0000-0002-1741-5486]
Tubau, Fe [0000-0002-6416-6451]
Giard, Jean-Christophe [0000-0001-8588-2732]
Cattoir, Vincent [0000-0003-4026-9912]
Ardanuy, Carmen [0000-0003-0225-607X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv GAS
L phenotype
LS(A)P phenotype
Streptococcus pyogenes
lnu(B)
lsa(E)
topic GAS
L phenotype
LS(A)P phenotype
Streptococcus pyogenes
lnu(B)
lsa(E)
description 19 p.-1 fig.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/192378
url http://hdl.handle.net/10261/192378
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ijantimicag.2019.08.019
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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