Molecular markers of endometrial carcinoma detected in uterine aspirates.

Endometrial cancer (EC) is the most frequent of the invasive tumors of the female genital tract. Although usually detected in its initial stages, a 20% of the patients present with advanced disease. To date, no characterized molecular marker has been validated for the diagnosis of EC. In addition, n...

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Autores: Colas, Eva, Perez, Cristina, Cabrera, Silvia, Pedrola, Nuria, Monge, Marta, Castellvi, Josep, Eyzaguirre, Fernando, Gregorio, Jesus, Ruiz, Anna, Llaurado, Marta, Rigau, Marina, Garcia, Marta, Ertekin, Tug\cce Tugce, Montes, Melania, López López, Rafael, Carreras, Ramon, Xercavins, Jordi, Ortega, Alicia, Maes, Tamara, Rosell, Elisabet, Doll, Andreas, Abal Posada, Miguel, Reventos, Jaume, Gil-Moreno, Antonio
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/5824
Acceso en línea:http://hdl.handle.net/20.500.11940/5824
Access Level:acceso abierto
Palabra clave:Female
Humans
Reproducibility of Results
Biomarkers, Tumor
Gene Expression Profiling
Body Fluids
Endometrial Neoplasms
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spelling Molecular markers of endometrial carcinoma detected in uterine aspirates.Colas, EvaPerez, CristinaCabrera, SilviaPedrola, NuriaMonge, MartaCastellvi, JosepEyzaguirre, FernandoGregorio, JesusRuiz, AnnaLlaurado, MartaRigau, MarinaGarcia, MartaErtekin, Tug\cce TugceMontes, MelaniaLópez López, RafaelCarreras, RamonXercavins, JordiOrtega, AliciaMaes, TamaraRosell, ElisabetDoll, AndreasAbal Posada, MiguelReventos, JaumeGil-Moreno, AntonioFemaleHumansReproducibility of ResultsBiomarkers, TumorGene Expression ProfilingBody FluidsEndometrial NeoplasmsEndometrial cancer (EC) is the most frequent of the invasive tumors of the female genital tract. Although usually detected in its initial stages, a 20% of the patients present with advanced disease. To date, no characterized molecular marker has been validated for the diagnosis of EC. In addition, new methods for prognosis and classification of EC are needed to combat this deadly disease. We thus aimed to identify new molecular markers of EC and to evaluate their validity on endometrial aspirates. Gene expression screening on 52 carcinoma samples and series of real-time quantitative PCR validation on 19 paired carcinomas and normal tissue samples and on 50 carcinoma and noncarcinoma uterine aspirates were performed to identify and validate potential biomarkers of EC. Candidate markers were further confirmed at the protein level by immunohistochemistry and Western blot. We identified ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, SOCS2 and DCN as differentially expressed in ECs. Furthermore, the differential expression of these biomarkers in primary endometrial tumors is correlated to their expression level in corresponding uterine fluid samples. Finally, these biomarkers significantly identified EC with area under the receiver-operating-characteristic values ranging from 0.74 to 0.95 in uterine aspirates. Interestingly, analogous values were found among initial stages. We present the discovery of molecular biomarkers of EC and describe their utility in uterine aspirates. These findings represent the basis for the development of a highly sensitive and specific minimally invasive method for screening ECs.2011info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.11940/5824reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Inglésinfo:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/58242026-06-12T08:40:47Z
dc.title.none.fl_str_mv Molecular markers of endometrial carcinoma detected in uterine aspirates.
title Molecular markers of endometrial carcinoma detected in uterine aspirates.
spellingShingle Molecular markers of endometrial carcinoma detected in uterine aspirates.
Colas, Eva
Female
Humans
Reproducibility of Results
Biomarkers, Tumor
Gene Expression Profiling
Body Fluids
Endometrial Neoplasms
title_short Molecular markers of endometrial carcinoma detected in uterine aspirates.
title_full Molecular markers of endometrial carcinoma detected in uterine aspirates.
title_fullStr Molecular markers of endometrial carcinoma detected in uterine aspirates.
title_full_unstemmed Molecular markers of endometrial carcinoma detected in uterine aspirates.
title_sort Molecular markers of endometrial carcinoma detected in uterine aspirates.
dc.creator.none.fl_str_mv Colas, Eva
Perez, Cristina
Cabrera, Silvia
Pedrola, Nuria
Monge, Marta
Castellvi, Josep
Eyzaguirre, Fernando
Gregorio, Jesus
Ruiz, Anna
Llaurado, Marta
Rigau, Marina
Garcia, Marta
Ertekin, Tug\cce Tugce
Montes, Melania
López López, Rafael
Carreras, Ramon
Xercavins, Jordi
Ortega, Alicia
Maes, Tamara
Rosell, Elisabet
Doll, Andreas
Abal Posada, Miguel
Reventos, Jaume
Gil-Moreno, Antonio
author Colas, Eva
author_facet Colas, Eva
Perez, Cristina
Cabrera, Silvia
Pedrola, Nuria
Monge, Marta
Castellvi, Josep
Eyzaguirre, Fernando
Gregorio, Jesus
Ruiz, Anna
Llaurado, Marta
Rigau, Marina
Garcia, Marta
Ertekin, Tug\cce Tugce
Montes, Melania
López López, Rafael
Carreras, Ramon
Xercavins, Jordi
Ortega, Alicia
Maes, Tamara
Rosell, Elisabet
Doll, Andreas
Abal Posada, Miguel
Reventos, Jaume
Gil-Moreno, Antonio
author_role author
author2 Perez, Cristina
Cabrera, Silvia
Pedrola, Nuria
Monge, Marta
Castellvi, Josep
Eyzaguirre, Fernando
Gregorio, Jesus
Ruiz, Anna
Llaurado, Marta
Rigau, Marina
Garcia, Marta
Ertekin, Tug\cce Tugce
Montes, Melania
López López, Rafael
Carreras, Ramon
Xercavins, Jordi
Ortega, Alicia
Maes, Tamara
Rosell, Elisabet
Doll, Andreas
Abal Posada, Miguel
Reventos, Jaume
Gil-Moreno, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Female
Humans
Reproducibility of Results
Biomarkers, Tumor
Gene Expression Profiling
Body Fluids
Endometrial Neoplasms
topic Female
Humans
Reproducibility of Results
Biomarkers, Tumor
Gene Expression Profiling
Body Fluids
Endometrial Neoplasms
description Endometrial cancer (EC) is the most frequent of the invasive tumors of the female genital tract. Although usually detected in its initial stages, a 20% of the patients present with advanced disease. To date, no characterized molecular marker has been validated for the diagnosis of EC. In addition, new methods for prognosis and classification of EC are needed to combat this deadly disease. We thus aimed to identify new molecular markers of EC and to evaluate their validity on endometrial aspirates. Gene expression screening on 52 carcinoma samples and series of real-time quantitative PCR validation on 19 paired carcinomas and normal tissue samples and on 50 carcinoma and noncarcinoma uterine aspirates were performed to identify and validate potential biomarkers of EC. Candidate markers were further confirmed at the protein level by immunohistochemistry and Western blot. We identified ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, SOCS2 and DCN as differentially expressed in ECs. Furthermore, the differential expression of these biomarkers in primary endometrial tumors is correlated to their expression level in corresponding uterine fluid samples. Finally, these biomarkers significantly identified EC with area under the receiver-operating-characteristic values ranging from 0.74 to 0.95 in uterine aspirates. Interestingly, analogous values were found among initial stages. We present the discovery of molecular biomarkers of EC and describe their utility in uterine aspirates. These findings represent the basis for the development of a highly sensitive and specific minimally invasive method for screening ECs.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.11940/5824
url http://hdl.handle.net/20.500.11940/5824
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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