Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle

Glucose constitutes a major fuel for the heart, and high glucose uptake during fetal development is coincident with the highest level of expression of the glucose transporter GLUT-1 during life. We have previously reported that GLUT-1 is repressed perinatally in rat heart, and GLUT-4, which shows a...

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Autores: Santalucía Albi, Tomàs, Boheler, Kenneth R., Brand, Nigel J., Sahye, Una, Fandos Espallargas, César, Viñals Canals, Francesc, Ferré, Josep, Testar, Xavier, Palacín Prieto, Manuel, Zorzano Olarte, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1999
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177079
Acceso en línea:https://hdl.handle.net/2445/177079
Access Level:acceso abierto
Palabra clave:Monosacàrids
Proteïnes
Músculs
Metabolisme
Miocardi
Monosaccharides
Proteins
Muscles
Metabolism
Myocardium
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repository_id_str
spelling Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscleSantalucía Albi, TomàsBoheler, Kenneth R.Brand, Nigel J.Sahye, UnaFandos Espallargas, CésarViñals Canals, FrancescFerré, JosepTestar, XavierPalacín Prieto, ManuelZorzano Olarte, AntonioMonosacàridsProteïnesMúsculsMetabolismeMiocardiMonosaccharidesProteinsMusclesMetabolismMyocardiumGlucose constitutes a major fuel for the heart, and high glucose uptake during fetal development is coincident with the highest level of expression of the glucose transporter GLUT-1 during life. We have previously reported that GLUT-1 is repressed perinatally in rat heart, and GLUT-4, which shows a low level of expression in the fetal stage, becomes the main glucose transporter in the adult. Here, we show that the perinatal expression of GLUT-1 and GLUT-4 glucose transporters in heart is controlled directly at the level of gene transcription. Transient transfection assays show that the -99/-33 fragment of the GLUT-1 gene is sufficient to drive transcriptional activity in rat neonatal cardiomyocytes. Electrophoretic mobility shift assays demonstrate that the transcription factor Sp1, a trans-activator of GLUT-1 promoter, binds to the -102/-82 region of GLUT-1 promoter during the fetal state but not during adulthood. Mutation of the Sp1 site in this region demonstrates that Sp1 is essential for maintaining a high transcriptional activity in cardiac myocytes. Sp1 is markedly down-regulated both in heart and in skeletal muscle during neonatal life, suggesting an active role for Sp1 in the regulation of GLUT-1 transcription. In all, these results indicate that the expression of GLUT-1 and GLUT-4 in heart during perinatal development is largely controlled at a transcriptional level by mechanisms that might be related to hyperplasia and that are independent from the signals that trigger cell hypertrophy in the developing heart. Furthermore, our results provide the first functional insight into the mechanisms regulating muscle GLUT-1 gene expression in a live animal.American Society for Biochemistry and Molecular Biology1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177079Articles publicats en revistes (Infermeria Fonamental i Clínica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1074/jbc.274.25.17626Journal of Biological Chemistry, 1999, vol. 274, num. 25, p. 17626-17634https://doi.org/10.1074/jbc.274.25.17626(c) American Society for Biochemistry and Molecular Biology, 1999info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1770792026-05-27T06:46:51Z
dc.title.none.fl_str_mv Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
title Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
spellingShingle Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
Santalucía Albi, Tomàs
Monosacàrids
Proteïnes
Músculs
Metabolisme
Miocardi
Monosaccharides
Proteins
Muscles
Metabolism
Myocardium
title_short Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
title_full Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
title_fullStr Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
title_full_unstemmed Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
title_sort Factors involved in GLUT1 glucose transporter gene transcription in cardiacmuscle
dc.creator.none.fl_str_mv Santalucía Albi, Tomàs
Boheler, Kenneth R.
Brand, Nigel J.
Sahye, Una
Fandos Espallargas, César
Viñals Canals, Francesc
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author Santalucía Albi, Tomàs
author_facet Santalucía Albi, Tomàs
Boheler, Kenneth R.
Brand, Nigel J.
Sahye, Una
Fandos Espallargas, César
Viñals Canals, Francesc
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author_role author
author2 Boheler, Kenneth R.
Brand, Nigel J.
Sahye, Una
Fandos Espallargas, César
Viñals Canals, Francesc
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Monosacàrids
Proteïnes
Músculs
Metabolisme
Miocardi
Monosaccharides
Proteins
Muscles
Metabolism
Myocardium
topic Monosacàrids
Proteïnes
Músculs
Metabolisme
Miocardi
Monosaccharides
Proteins
Muscles
Metabolism
Myocardium
description Glucose constitutes a major fuel for the heart, and high glucose uptake during fetal development is coincident with the highest level of expression of the glucose transporter GLUT-1 during life. We have previously reported that GLUT-1 is repressed perinatally in rat heart, and GLUT-4, which shows a low level of expression in the fetal stage, becomes the main glucose transporter in the adult. Here, we show that the perinatal expression of GLUT-1 and GLUT-4 glucose transporters in heart is controlled directly at the level of gene transcription. Transient transfection assays show that the -99/-33 fragment of the GLUT-1 gene is sufficient to drive transcriptional activity in rat neonatal cardiomyocytes. Electrophoretic mobility shift assays demonstrate that the transcription factor Sp1, a trans-activator of GLUT-1 promoter, binds to the -102/-82 region of GLUT-1 promoter during the fetal state but not during adulthood. Mutation of the Sp1 site in this region demonstrates that Sp1 is essential for maintaining a high transcriptional activity in cardiac myocytes. Sp1 is markedly down-regulated both in heart and in skeletal muscle during neonatal life, suggesting an active role for Sp1 in the regulation of GLUT-1 transcription. In all, these results indicate that the expression of GLUT-1 and GLUT-4 in heart during perinatal development is largely controlled at a transcriptional level by mechanisms that might be related to hyperplasia and that are independent from the signals that trigger cell hypertrophy in the developing heart. Furthermore, our results provide the first functional insight into the mechanisms regulating muscle GLUT-1 gene expression in a live animal.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177079
url https://hdl.handle.net/2445/177079
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.274.25.17626
Journal of Biological Chemistry, 1999, vol. 274, num. 25, p. 17626-17634
https://doi.org/10.1074/jbc.274.25.17626
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 1999
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 1999
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Infermeria Fonamental i Clínica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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