Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon

The human immunodeficiency virus type 1 (HIV-1) regulator Tat is essential for viral replication because it achieves complete elongation of viral transcripts. Tat can be released to the extracellular space and taken up by adjacent cells, exerting profound cytoskeleton rearrangements that lead to apo...

Descripción completa

Detalles Bibliográficos
Autores: Lopez-Huertas, Maria Rosa, Callejas, S., Abia, David, Mateos, Elena, Dopazo, A., Alcamí, José, Coiras, Mayte
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6965
Acceso en línea:http://hdl.handle.net/20.500.12105/6965
Access Level:acceso abierto
Palabra clave:Cell Proliferation
Chemotaxis
Computational Biology
Cytoskeleton
Exons
Gene Expression
HIV-1
Humans
Jurkat Cells
Models, Molecular
Receptors, Cell Surface
Transcriptional Activation
tat Gene Products, Human Immunodeficiency Virus
id ES_8a79165ac708193d90ec558c7d101bf0
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/6965
network_acronym_str ES
network_name_str España
repository_id_str
spelling Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exonLopez-Huertas, Maria RosaCallejas, S.Abia, DavidMateos, ElenaDopazo, A.Alcamí, JoséCoiras, MayteCell ProliferationChemotaxisComputational BiologyCytoskeletonExonsGene ExpressionHIV-1HumansJurkat CellsModels, MolecularReceptors, Cell SurfaceTranscriptional Activationtat Gene Products, Human Immunodeficiency VirusThe human immunodeficiency virus type 1 (HIV-1) regulator Tat is essential for viral replication because it achieves complete elongation of viral transcripts. Tat can be released to the extracellular space and taken up by adjacent cells, exerting profound cytoskeleton rearrangements that lead to apoptosis. In contrast, intracellular Tat has been described as protector from apoptosis. Tat gene is composed by two coding exons that yield a protein of 101 amino acids (aa). First exon (1-72aa) is sufficient for viral transcript elongation and second exon (73-101 aa) appears to contribute to non-transcriptional functions. We observed that Jurkat cells stably expressing intracellular Tat101 showed gene expression deregulation 4-fold higher than cells expressing Tat72. Functional experiments were performed to evaluate the effect of this deregulation. First, NF-kappaB-, NF-AT- and Sp1-dependent transcriptional activities were greatly enhanced in Jurkat-Tat101, whereas Tat72 induced milder but efficient activation. Second, cytoskeleton-related functions as cell morphology, proliferation, chemotaxis, polarization and actin polymerization were deeply altered in Jurkat-Tat101, but not in Jurkat-Tat72. Finally, expression of several cell surface receptors was dramatically impaired by intracellular Tat101 but not by Tat72. Consequently, these modifications were greatly dependent on Tat second exon and they could be related to the anergy observed in HIV-1-infected T cells.Oxford University PressPlan Nacional sobre el Sida (España)Fundación para la Investigación y la Prevención del Sida en EspañaComunidad de Madrid (España)Instituto de Salud Carlos IIIMinisterio de Ciencia e Innovación (España)20182018-12-2720102010-02-0520102010-02-05research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/6965reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES MVI1434 05–5ES PI040614 Not availableES PI0808752 Not availableES BIO2008-04384 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/69652026-06-12T12:43:37Z
dc.title.none.fl_str_mv Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
title Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
spellingShingle Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
Lopez-Huertas, Maria Rosa
Cell Proliferation
Chemotaxis
Computational Biology
Cytoskeleton
Exons
Gene Expression
HIV-1
Humans
Jurkat Cells
Models, Molecular
Receptors, Cell Surface
Transcriptional Activation
tat Gene Products, Human Immunodeficiency Virus
title_short Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
title_full Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
title_fullStr Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
title_full_unstemmed Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
title_sort Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
dc.creator.none.fl_str_mv Lopez-Huertas, Maria Rosa
Callejas, S.
Abia, David
Mateos, Elena
Dopazo, A.
Alcamí, José
Coiras, Mayte
author Lopez-Huertas, Maria Rosa
author_facet Lopez-Huertas, Maria Rosa
Callejas, S.
Abia, David
Mateos, Elena
Dopazo, A.
Alcamí, José
Coiras, Mayte
author_role author
author2 Callejas, S.
Abia, David
Mateos, Elena
Dopazo, A.
Alcamí, José
Coiras, Mayte
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Plan Nacional sobre el Sida (España)
Fundación para la Investigación y la Prevención del Sida en España
Comunidad de Madrid (España)
Instituto de Salud Carlos III
Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Cell Proliferation
Chemotaxis
Computational Biology
Cytoskeleton
Exons
Gene Expression
HIV-1
Humans
Jurkat Cells
Models, Molecular
Receptors, Cell Surface
Transcriptional Activation
tat Gene Products, Human Immunodeficiency Virus
topic Cell Proliferation
Chemotaxis
Computational Biology
Cytoskeleton
Exons
Gene Expression
HIV-1
Humans
Jurkat Cells
Models, Molecular
Receptors, Cell Surface
Transcriptional Activation
tat Gene Products, Human Immunodeficiency Virus
description The human immunodeficiency virus type 1 (HIV-1) regulator Tat is essential for viral replication because it achieves complete elongation of viral transcripts. Tat can be released to the extracellular space and taken up by adjacent cells, exerting profound cytoskeleton rearrangements that lead to apoptosis. In contrast, intracellular Tat has been described as protector from apoptosis. Tat gene is composed by two coding exons that yield a protein of 101 amino acids (aa). First exon (1-72aa) is sufficient for viral transcript elongation and second exon (73-101 aa) appears to contribute to non-transcriptional functions. We observed that Jurkat cells stably expressing intracellular Tat101 showed gene expression deregulation 4-fold higher than cells expressing Tat72. Functional experiments were performed to evaluate the effect of this deregulation. First, NF-kappaB-, NF-AT- and Sp1-dependent transcriptional activities were greatly enhanced in Jurkat-Tat101, whereas Tat72 induced milder but efficient activation. Second, cytoskeleton-related functions as cell morphology, proliferation, chemotaxis, polarization and actin polymerization were deeply altered in Jurkat-Tat101, but not in Jurkat-Tat72. Finally, expression of several cell surface receptors was dramatically impaired by intracellular Tat101 but not by Tat72. Consequently, these modifications were greatly dependent on Tat second exon and they could be related to the anergy observed in HIV-1-infected T cells.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-02-05
2010
2010-02-05
2018
2018-12-27
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/6965
url http://hdl.handle.net/20.500.12105/6965
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES MVI1434 05–5
ES PI040614 Not available
ES PI0808752 Not available
ES BIO2008-04384 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412718906179585
score 15,812429

Similares en LA Referencia