Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast

An important question in cell biology is how cellular organelles partition during cell division. In organisms undergoing closed mitosis, the elongation of an intranuclear spindle drives nuclear division, generating two identically sized nuclei [1, 2]. However, how the site of nuclear division is det...

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Detalhes bibliográficos
Autores: Expósito Serrano, María, Sánchez Molina, Ana, Gallardo Palomo, Paola, Salas-Pino, Silvia, Daga, Rafael
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:rio.upo.es:10433/25241
Acesso em linha:https://hdl.handle.net/10433/25241
Access Level:acceso abierto
Palavra-chave:Mitosis
Importin α
Nuclear pore complex
Nuclear envelope
Nuclear envelope division
Central spindle
Midzone membrane domain
ER tubules
Endomembrane sorting
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spelling Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission YeastExpósito Serrano, MaríaSánchez Molina, AnaGallardo Palomo, PaolaSalas-Pino, SilviaDaga, RafaelMitosisImportin αNuclear pore complexNuclear envelopeNuclear envelope divisionCentral spindleMidzone membrane domainER tubulesEndomembrane sortingAn important question in cell biology is how cellular organelles partition during cell division. In organisms undergoing closed mitosis, the elongation of an intranuclear spindle drives nuclear division, generating two identically sized nuclei [1, 2]. However, how the site of nuclear division is determined and the underlying mechanism driving nuclear envelope (NE) fission remain largely unknown. Here, using the fission yeast, we show that the microtubule bundler Ase1/PRC1 at the spindle midzone is required for the local concentration of nuclear pore complexes (NPCs) in the region of the NE in contact with the central spindle. As the spindle elongates during anaphase B, components of these NPCs are sequentially eliminated, and this is accompanied by the local remodeling of the NE. These two events lead to the eventual removal of NPCs and nuclear division. In the absence of importin a, NPCs remain stable in this region and no event of NE remodeling is observed. Consequently, cells fail to undergo nuclear division. Thus, our results highlight a new role of the central spindle as a spatial cue that determines the site of nuclear division and point to NPC removal as the triggering event.Cell Press20252025-12-1720202020-08-1720202020-08-17journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10433/25241reponame:RIO. Repositorio Institucional Olavideinstname:Universidad Pablo de Olavide (UPO)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099849-B-I00 ARQUITECTURA Y MECANICA NUCLEAR EN LA LEVADURA DE FISIONAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099849-B-I00 ARQUITECTURA Y MECANICA NUCLEAR EN LA LEVADURA DE FISIONopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:rio.upo.es:10433/252412026-06-13T12:46:27Z
dc.title.none.fl_str_mv Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
title Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
spellingShingle Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
Expósito Serrano, María
Mitosis
Importin α
Nuclear pore complex
Nuclear envelope
Nuclear envelope division
Central spindle
Midzone membrane domain
ER tubules
Endomembrane sorting
title_short Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
title_full Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
title_fullStr Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
title_full_unstemmed Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
title_sort Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
dc.creator.none.fl_str_mv Expósito Serrano, María
Sánchez Molina, Ana
Gallardo Palomo, Paola
Salas-Pino, Silvia
Daga, Rafael
author Expósito Serrano, María
author_facet Expósito Serrano, María
Sánchez Molina, Ana
Gallardo Palomo, Paola
Salas-Pino, Silvia
Daga, Rafael
author_role author
author2 Sánchez Molina, Ana
Gallardo Palomo, Paola
Salas-Pino, Silvia
Daga, Rafael
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Mitosis
Importin α
Nuclear pore complex
Nuclear envelope
Nuclear envelope division
Central spindle
Midzone membrane domain
ER tubules
Endomembrane sorting
topic Mitosis
Importin α
Nuclear pore complex
Nuclear envelope
Nuclear envelope division
Central spindle
Midzone membrane domain
ER tubules
Endomembrane sorting
description An important question in cell biology is how cellular organelles partition during cell division. In organisms undergoing closed mitosis, the elongation of an intranuclear spindle drives nuclear division, generating two identically sized nuclei [1, 2]. However, how the site of nuclear division is determined and the underlying mechanism driving nuclear envelope (NE) fission remain largely unknown. Here, using the fission yeast, we show that the microtubule bundler Ase1/PRC1 at the spindle midzone is required for the local concentration of nuclear pore complexes (NPCs) in the region of the NE in contact with the central spindle. As the spindle elongates during anaphase B, components of these NPCs are sequentially eliminated, and this is accompanied by the local remodeling of the NE. These two events lead to the eventual removal of NPCs and nuclear division. In the absence of importin a, NPCs remain stable in this region and no event of NE remodeling is observed. Consequently, cells fail to undergo nuclear division. Thus, our results highlight a new role of the central spindle as a spatial cue that determines the site of nuclear division and point to NPC removal as the triggering event.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-08-17
2020
2020-08-17
2025
2025-12-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10433/25241
url https://hdl.handle.net/10433/25241
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099849-B-I00 ARQUITECTURA Y MECANICA NUCLEAR EN LA LEVADURA DE FISION
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099849-B-I00 ARQUITECTURA Y MECANICA NUCLEAR EN LA LEVADURA DE FISION
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:RIO. Repositorio Institucional Olavide
instname:Universidad Pablo de Olavide (UPO)
instname_str Universidad Pablo de Olavide (UPO)
reponame_str RIO. Repositorio Institucional Olavide
collection RIO. Repositorio Institucional Olavide
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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