Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study

Stage II colorectal cancer (CRC) recurrence remains a clinical problem. Some of these patients are true stage III CRC with a pN0 pathology stage. This large prospective multicentre cohort study aimed at evaluating the diagnostic ability of lymph node (LN) cytology smears to perform the pN stage and...

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Autores: Diaz Mercedes, Sherley, Archilla Sanz, Iván, Lahoz, Sara, Rodrigo Calvo, María Teresa, López Prades, Sandra, Tarragona, Jordi, Landolfi, Stefania, Concha, Ángel, Machado, Isidro, Maurel Santasusana, Joan, Chic Ruché, Núria, Castells, Antoni, Balaguer Prunés, Francesc, Camps, Jordi, Cuatrecasas Freixas, Miriam
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/209370
Acesso em linha:https://hdl.handle.net/2445/209370
Access Level:Acceso aberto
Palavra-chave:Nodes limfàtics
Citologia
Càncer colorectal
Lymph nodes
Cytology
Colorectal cancer
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spelling Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre StudyDiaz Mercedes, SherleyArchilla Sanz, IvánLahoz, SaraRodrigo Calvo, María TeresaLópez Prades, Sandra Tarragona, JordiLandolfi, StefaniaConcha, ÁngelMachado, IsidroMaurel Santasusana, JoanChic Ruché, NúriaCastells, AntoniBalaguer Prunés, FrancescCamps, JordiCuatrecasas Freixas, MiriamNodes limfàticsCitologiaCàncer colorectalLymph nodesCytologyColorectal cancerStage II colorectal cancer (CRC) recurrence remains a clinical problem. Some of these patients are true stage III CRC with a pN0 pathology stage. This large prospective multicentre cohort study aimed at evaluating the diagnostic ability of lymph node (LN) cytology smears to perform the pN stage and compare it with the conventional haematoxylin and eosin (H&E) pathology pN stage. Additionally, we used the One-Step Nucleic Acid Amplification (OSNA), a high-sensitive molecular method of LN staging. A total of 3936 fresh LNs from 217 CRC surgical specimens were examined by three methods, H&E, LN cytology smears, and OSNA. H&E detected 29% of patients with positive LNs, cytology smears 35%, and OSNA 33.2% (p < 0.0001). H&E and cytology concordantly classified 92.2% of tumours, and 88.5% between OSNA and H&E. Cytology had 96.8% sensitivity and 90.3% specificity to discriminate positive/negative patients compared to H&E (p = 0.004), and 87.3% sensitivity and 89% specificity when compared to OSNA (p = 0.56). Patients with positive LNs detected by any of the three methods had significantly worse disease-free and overall survival. We conclude that pN stage accuracy for detecting positive LNs is superior with LN cytological smears than with conventional H&E, which would enable a better pN stage and management of early-stage CRC patients.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/209370Articles publicats en revistes (Medicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/cancers14246072Cancers, 2022, vol. 14, num.24https://doi.org/10.3390/cancers14246072cc-by (c) Diaz-Mercedes, S. et al., 2022http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2093702026-05-27T06:46:51Z
dc.title.none.fl_str_mv Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
title Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
spellingShingle Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
Diaz Mercedes, Sherley
Nodes limfàtics
Citologia
Càncer colorectal
Lymph nodes
Cytology
Colorectal cancer
title_short Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
title_full Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
title_fullStr Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
title_full_unstemmed Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
title_sort Cytology Smears: An Enhanced Alternative Method for Colorectal Cancer pN Stage-A Multicentre Study
dc.creator.none.fl_str_mv Diaz Mercedes, Sherley
Archilla Sanz, Iván
Lahoz, Sara
Rodrigo Calvo, María Teresa
López Prades, Sandra
Tarragona, Jordi
Landolfi, Stefania
Concha, Ángel
Machado, Isidro
Maurel Santasusana, Joan
Chic Ruché, Núria
Castells, Antoni
Balaguer Prunés, Francesc
Camps, Jordi
Cuatrecasas Freixas, Miriam
author Diaz Mercedes, Sherley
author_facet Diaz Mercedes, Sherley
Archilla Sanz, Iván
Lahoz, Sara
Rodrigo Calvo, María Teresa
López Prades, Sandra
Tarragona, Jordi
Landolfi, Stefania
Concha, Ángel
Machado, Isidro
Maurel Santasusana, Joan
Chic Ruché, Núria
Castells, Antoni
Balaguer Prunés, Francesc
Camps, Jordi
Cuatrecasas Freixas, Miriam
author_role author
author2 Archilla Sanz, Iván
Lahoz, Sara
Rodrigo Calvo, María Teresa
López Prades, Sandra
Tarragona, Jordi
Landolfi, Stefania
Concha, Ángel
Machado, Isidro
Maurel Santasusana, Joan
Chic Ruché, Núria
Castells, Antoni
Balaguer Prunés, Francesc
Camps, Jordi
Cuatrecasas Freixas, Miriam
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nodes limfàtics
Citologia
Càncer colorectal
Lymph nodes
Cytology
Colorectal cancer
topic Nodes limfàtics
Citologia
Càncer colorectal
Lymph nodes
Cytology
Colorectal cancer
description Stage II colorectal cancer (CRC) recurrence remains a clinical problem. Some of these patients are true stage III CRC with a pN0 pathology stage. This large prospective multicentre cohort study aimed at evaluating the diagnostic ability of lymph node (LN) cytology smears to perform the pN stage and compare it with the conventional haematoxylin and eosin (H&E) pathology pN stage. Additionally, we used the One-Step Nucleic Acid Amplification (OSNA), a high-sensitive molecular method of LN staging. A total of 3936 fresh LNs from 217 CRC surgical specimens were examined by three methods, H&E, LN cytology smears, and OSNA. H&E detected 29% of patients with positive LNs, cytology smears 35%, and OSNA 33.2% (p < 0.0001). H&E and cytology concordantly classified 92.2% of tumours, and 88.5% between OSNA and H&E. Cytology had 96.8% sensitivity and 90.3% specificity to discriminate positive/negative patients compared to H&E (p = 0.004), and 87.3% sensitivity and 89% specificity when compared to OSNA (p = 0.56). Patients with positive LNs detected by any of the three methods had significantly worse disease-free and overall survival. We conclude that pN stage accuracy for detecting positive LNs is superior with LN cytological smears than with conventional H&E, which would enable a better pN stage and management of early-stage CRC patients.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/209370
url https://hdl.handle.net/2445/209370
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cancers14246072
Cancers, 2022, vol. 14, num.24
https://doi.org/10.3390/cancers14246072
dc.rights.none.fl_str_mv cc-by (c) Diaz-Mercedes, S. et al., 2022
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Diaz-Mercedes, S. et al., 2022
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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