Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7

Incubation of carboxydotrophs at 37. °C provides optimal conditions for their growth. However, a fast accumulation of organic acids, specifically acetate, during the exponential growth phase may result in low alcohol production and substrate consumption due to a phenomenon known as "acid crash&...

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Autores: Ramió Pujol, Sara, Ganigué Pagès, Ramon, Bañeras Vives, Lluís, Colprim Galceran, Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/10962
Acceso en línea:http://hdl.handle.net/10256/10962
Access Level:acceso embargado
Palabra clave:Biocombustibles
Biocarburants
Gasos
Gases
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spelling Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7Ramió Pujol, SaraGanigué Pagès, RamonBañeras Vives, LluísColprim Galceran, JesúsBiocombustiblesBiocarburantsGasosGasesIncubation of carboxydotrophs at 37. °C provides optimal conditions for their growth. However, a fast accumulation of organic acids, specifically acetate, during the exponential growth phase may result in low alcohol production and substrate consumption due to a phenomenon known as "acid crash". The present work investigates growth and productivity of Clostridium carboxidivorans P7 at two incubation temperatures. At 37. °C the culture was not able to override the "acid crash", resulting in low ethanol titers (1.56. mM). On the other hand, lower metabolic rates at 25. °C enhanced ethanol and butanol production (32.1 and 14.5. mM, respectively). Moreover, at low temperatures, hexanol and caproic acid were also produced at significant concentrations, 8.21 and 9.02. mM respectively, among the highest values reported for P7. Our results demonstrate that production of biofuels with longer carbon chains molecules may be enhanced incubating syngas-fermenting acetogenic bacteria at sub-optimal temperaturesThe authors would like to thank the Generalitat de Catalunya (2013 FI-DGR) and Spanish Ministry (CTQ2014-53718-R and CTM2013-43454-R) for their financial support in this study. LEQUIA and IEA are recognized as consolidated research groups by the Generalitat de Catalunya (2014-SGR-1168 and 2014-SGR-2016). R.G. gratefully acknowledges support from Beatriu de Pinos fellowship (BP-2011-B) and FP7 Marie Curie Career Integration Grants (PCIG13-GA-2013-618593)ElsevierMinisterio de Economía y Competitividad (Espanya)infoinfo2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/10962http://hdl.handle.net/10256/10962© Bioresource Technology, 2015, vol. 192, p. 296-303Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2015.05.077info:eu-repo/semantics/altIdentifier/issn/0960-8524info:eu-repo/grantAgreement/MINECO//CTQ2014-53718-Rinfo:eu-repo/grantAgreement/MINECO//CTM2013-43454-Rinfo:eu-repo/grantAgreement/EC/FP7/618593Tots els drets reservatsinfo:eu-repo/semantics/embargoedAccessoai:recercat.cat:10256/109622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
title Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
spellingShingle Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
Ramió Pujol, Sara
Biocombustibles
Biocarburants
Gasos
Gases
title_short Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
title_full Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
title_fullStr Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
title_full_unstemmed Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
title_sort Incubation at 25°C prevents acid crash and enhances alcohol production in Clostridium carboxidivorans P7
dc.creator.none.fl_str_mv Ramió Pujol, Sara
Ganigué Pagès, Ramon
Bañeras Vives, Lluís
Colprim Galceran, Jesús
author Ramió Pujol, Sara
author_facet Ramió Pujol, Sara
Ganigué Pagès, Ramon
Bañeras Vives, Lluís
Colprim Galceran, Jesús
author_role author
author2 Ganigué Pagès, Ramon
Bañeras Vives, Lluís
Colprim Galceran, Jesús
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Biocombustibles
Biocarburants
Gasos
Gases
topic Biocombustibles
Biocarburants
Gasos
Gases
description Incubation of carboxydotrophs at 37. °C provides optimal conditions for their growth. However, a fast accumulation of organic acids, specifically acetate, during the exponential growth phase may result in low alcohol production and substrate consumption due to a phenomenon known as "acid crash". The present work investigates growth and productivity of Clostridium carboxidivorans P7 at two incubation temperatures. At 37. °C the culture was not able to override the "acid crash", resulting in low ethanol titers (1.56. mM). On the other hand, lower metabolic rates at 25. °C enhanced ethanol and butanol production (32.1 and 14.5. mM, respectively). Moreover, at low temperatures, hexanol and caproic acid were also produced at significant concentrations, 8.21 and 9.02. mM respectively, among the highest values reported for P7. Our results demonstrate that production of biofuels with longer carbon chains molecules may be enhanced incubating syngas-fermenting acetogenic bacteria at sub-optimal temperatures
publishDate 2015
dc.date.none.fl_str_mv 2015
info
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/10962
http://hdl.handle.net/10256/10962
url http://hdl.handle.net/10256/10962
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2015.05.077
info:eu-repo/semantics/altIdentifier/issn/0960-8524
info:eu-repo/grantAgreement/MINECO//CTQ2014-53718-R
info:eu-repo/grantAgreement/MINECO//CTM2013-43454-R
info:eu-repo/grantAgreement/EC/FP7/618593
dc.rights.none.fl_str_mv Tots els drets reservats
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Tots els drets reservats
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Bioresource Technology, 2015, vol. 192, p. 296-303
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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