Antiplatelet activity of isorhamnetin via mitochondrial regulation

With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to mai...

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Autores: Rodríguez Domínguez, Laura|||0000-0002-6669-1962, Badimon, Lina|||0000-0002-9162-2459, Méndez, D., Padró, Teresa|||0000-0003-1921-954X, Vilahur, Gemma|||0000-0002-2828-8873, Peña, Esther|||0000-0003-2750-0614, Carrasco, B., Vogel, Hermine|||0000-0003-0072-4239, Palomo, Iván, Fuentes, E.
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:271985
Acesso em linha:https://ddd.uab.cat/record/271985
https://dx.doi.org/urn:doi:10.3390/antiox10050666
Access Level:acceso abierto
Palavra-chave:Isorhamnetin
Flavonoids
Antithrombotic
Antiplatelet
Foods
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spelling Antiplatelet activity of isorhamnetin via mitochondrial regulationRodríguez Domínguez, Laura|||0000-0002-6669-1962Badimon, Lina|||0000-0002-9162-2459Méndez, D.Padró, Teresa|||0000-0003-1921-954XVilahur, Gemma|||0000-0002-2828-8873Peña, Esther|||0000-0003-2750-0614Carrasco, B.Vogel, Hermine|||0000-0003-0072-4239Palomo, IvánFuentes, E.IsorhamnetinFlavonoidsAntithromboticAntiplateletFoodsWith the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin's antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen-and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC) values of 8.1 ± 2.6 and 16.1 ± 11.1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin's antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin's effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/271985https://dx.doi.org/urn:doi:10.3390/antiox10050666reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2719852026-06-06T12:50:31Z
dc.title.none.fl_str_mv Antiplatelet activity of isorhamnetin via mitochondrial regulation
title Antiplatelet activity of isorhamnetin via mitochondrial regulation
spellingShingle Antiplatelet activity of isorhamnetin via mitochondrial regulation
Rodríguez Domínguez, Laura|||0000-0002-6669-1962
Isorhamnetin
Flavonoids
Antithrombotic
Antiplatelet
Foods
title_short Antiplatelet activity of isorhamnetin via mitochondrial regulation
title_full Antiplatelet activity of isorhamnetin via mitochondrial regulation
title_fullStr Antiplatelet activity of isorhamnetin via mitochondrial regulation
title_full_unstemmed Antiplatelet activity of isorhamnetin via mitochondrial regulation
title_sort Antiplatelet activity of isorhamnetin via mitochondrial regulation
dc.creator.none.fl_str_mv Rodríguez Domínguez, Laura|||0000-0002-6669-1962
Badimon, Lina|||0000-0002-9162-2459
Méndez, D.
Padró, Teresa|||0000-0003-1921-954X
Vilahur, Gemma|||0000-0002-2828-8873
Peña, Esther|||0000-0003-2750-0614
Carrasco, B.
Vogel, Hermine|||0000-0003-0072-4239
Palomo, Iván
Fuentes, E.
author Rodríguez Domínguez, Laura|||0000-0002-6669-1962
author_facet Rodríguez Domínguez, Laura|||0000-0002-6669-1962
Badimon, Lina|||0000-0002-9162-2459
Méndez, D.
Padró, Teresa|||0000-0003-1921-954X
Vilahur, Gemma|||0000-0002-2828-8873
Peña, Esther|||0000-0003-2750-0614
Carrasco, B.
Vogel, Hermine|||0000-0003-0072-4239
Palomo, Iván
Fuentes, E.
author_role author
author2 Badimon, Lina|||0000-0002-9162-2459
Méndez, D.
Padró, Teresa|||0000-0003-1921-954X
Vilahur, Gemma|||0000-0002-2828-8873
Peña, Esther|||0000-0003-2750-0614
Carrasco, B.
Vogel, Hermine|||0000-0003-0072-4239
Palomo, Iván
Fuentes, E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Isorhamnetin
Flavonoids
Antithrombotic
Antiplatelet
Foods
topic Isorhamnetin
Flavonoids
Antithrombotic
Antiplatelet
Foods
description With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin's antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen-and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC) values of 8.1 ± 2.6 and 16.1 ± 11.1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin's antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin's effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/271985
https://dx.doi.org/urn:doi:10.3390/antiox10050666
url https://ddd.uab.cat/record/271985
https://dx.doi.org/urn:doi:10.3390/antiox10050666
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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