Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine

Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of t...

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Autores: Barbosa Torralbo, José, Sanz Nebot, María Victoria, Benavente Moreno, Fernando J. (Julián), Giménez López, Estela
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/134938
Acceso en línea:https://hdl.handle.net/2445/134938
Access Level:acceso abierto
Palabra clave:Electroforesi capil·lar
Glicoproteïnes
Espectrometria de masses
Proteïnes
Capillary electrophoresis
Glycoproteins
Mass spectrometry
Proteins
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spelling Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicineBarbosa Torralbo, JoséSanz Nebot, María VictoriaBenavente Moreno, Fernando J. (Julián)Giménez López, EstelaElectroforesi capil·larGlicoproteïnesEspectrometria de massesProteïnesCapillary electrophoresisGlycoproteinsMass spectrometryProteinsGlycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms.2019201920062019info:eu-repo/semantics/article7 p.application/pdfhttps://hdl.handle.net/2445/134938Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Ingléshttp://dergi.fabad.org.tr/pdf/volum31/issue2/63-69.pdfFABAD Journal of Pharmaceutical Sciences, 2006, vol. 31, p. 63-69, 2006info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1349382026-05-29T05:05:01Z
dc.title.none.fl_str_mv Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
title Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
spellingShingle Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
Barbosa Torralbo, José
Electroforesi capil·lar
Glicoproteïnes
Espectrometria de masses
Proteïnes
Capillary electrophoresis
Glycoproteins
Mass spectrometry
Proteins
title_short Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
title_full Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
title_fullStr Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
title_full_unstemmed Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
title_sort Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
dc.creator.none.fl_str_mv Barbosa Torralbo, José
Sanz Nebot, María Victoria
Benavente Moreno, Fernando J. (Julián)
Giménez López, Estela
author Barbosa Torralbo, José
author_facet Barbosa Torralbo, José
Sanz Nebot, María Victoria
Benavente Moreno, Fernando J. (Julián)
Giménez López, Estela
author_role author
author2 Sanz Nebot, María Victoria
Benavente Moreno, Fernando J. (Julián)
Giménez López, Estela
author2_role author
author
author
dc.subject.none.fl_str_mv Electroforesi capil·lar
Glicoproteïnes
Espectrometria de masses
Proteïnes
Capillary electrophoresis
Glycoproteins
Mass spectrometry
Proteins
topic Electroforesi capil·lar
Glicoproteïnes
Espectrometria de masses
Proteïnes
Capillary electrophoresis
Glycoproteins
Mass spectrometry
Proteins
description Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms.
publishDate 2006
dc.date.none.fl_str_mv 2006
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/134938
url https://hdl.handle.net/2445/134938
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dergi.fabad.org.tr/pdf/volum31/issue2/63-69.pdf
FABAD Journal of Pharmaceutical Sciences, 2006, vol. 31, p. 63-69
dc.rights.none.fl_str_mv , 2006
info:eu-repo/semantics/openAccess
rights_invalid_str_mv , 2006
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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