Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants

We performed a detailed analysis of mouse cytochrome P450 2A5 (CYP2A5) expression by in situ hybridization (ISH) and immunohistochemistry (IHC) in the respiratory tissues of mice. The CYP2A5 mRNA and the corresponding protein co-localized at most sites and were predominantly detected in the olfactor...

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Autores: Piras, E, Franzen, A, Fernandez, EL, Bergstrom, U, Raffalli-Mathieu, F, Lang, M, Brittebo, EB
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p14272
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/14272
Access Level:acceso abierto
Palabra clave:CYP2A5
immunohistochemistry
in situ hybridization
olfactory toxicant
dichlobenil
methimazole
olfactory neuroepithelium
Bowman's glands
salivary gland
respiratory metaplasia
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spelling Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicantsPiras, EFranzen, AFernandez, ELBergstrom, URaffalli-Mathieu, FLang, MBrittebo, EBCYP2A5immunohistochemistryin situ hybridizationolfactory toxicantdichlobenilmethimazoleolfactory neuroepitheliumBowman's glandssalivary glandrespiratory metaplasiaWe performed a detailed analysis of mouse cytochrome P450 2A5 (CYP2A5) expression by in situ hybridization (ISH) and immunohistochemistry (IHC) in the respiratory tissues of mice. The CYP2A5 mRNA and the corresponding protein co-localized at most sites and were predominantly detected in the olfactory region, with an expression in sustentacular cells, Bowman's gland, and duct cells. In the respiratory and transitional epithelium there was no or only weak expression. The nasolacrimal duct and the excretory ducts of nasal and salivary glands displayed expression, whereas no expression occurred in the acini. There was decreasing expression along the epithelial linings of the trachea and lower respiratory tract, whereas no expression occurred in the alveoli. The hepatic CYP2A5 inducers pyrazole and phenobarbital neither changed the CYP2A5 expression pattern nor damaged the olfactory mucosa. In contrast, the olfactory toxicants dichlobenil and methimazole induced characteristic changes. The damaged Bowman's glands displayed no expression, whereas the damaged epithelium expressed the enzyme. The CYP2A5 expression pattern is in accordance with previously reported localization of protein and DNA adducts and the toxicity of some CYP2A5 substrates. This suggests that CYP2A5 is an important determinant for the susceptibility of the nasal and respiratory epithelia to protoxicants and procarcinogens.SAGE PUBLICATIONS LTD2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/14272JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRYISSN: 00221554ISSNe: 15515044reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p142722026-06-11T12:45:17Z
dc.title.none.fl_str_mv Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
title Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
spellingShingle Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
Piras, E
CYP2A5
immunohistochemistry
in situ hybridization
olfactory toxicant
dichlobenil
methimazole
olfactory neuroepithelium
Bowman's glands
salivary gland
respiratory metaplasia
title_short Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
title_full Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
title_fullStr Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
title_full_unstemmed Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
title_sort Cell-specific expression of CYP2A5 in the mouse respiratory tract: Effects of olfactory toxicants
dc.creator.none.fl_str_mv Piras, E
Franzen, A
Fernandez, EL
Bergstrom, U
Raffalli-Mathieu, F
Lang, M
Brittebo, EB
author Piras, E
author_facet Piras, E
Franzen, A
Fernandez, EL
Bergstrom, U
Raffalli-Mathieu, F
Lang, M
Brittebo, EB
author_role author
author2 Franzen, A
Fernandez, EL
Bergstrom, U
Raffalli-Mathieu, F
Lang, M
Brittebo, EB
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CYP2A5
immunohistochemistry
in situ hybridization
olfactory toxicant
dichlobenil
methimazole
olfactory neuroepithelium
Bowman's glands
salivary gland
respiratory metaplasia
topic CYP2A5
immunohistochemistry
in situ hybridization
olfactory toxicant
dichlobenil
methimazole
olfactory neuroepithelium
Bowman's glands
salivary gland
respiratory metaplasia
description We performed a detailed analysis of mouse cytochrome P450 2A5 (CYP2A5) expression by in situ hybridization (ISH) and immunohistochemistry (IHC) in the respiratory tissues of mice. The CYP2A5 mRNA and the corresponding protein co-localized at most sites and were predominantly detected in the olfactory region, with an expression in sustentacular cells, Bowman's gland, and duct cells. In the respiratory and transitional epithelium there was no or only weak expression. The nasolacrimal duct and the excretory ducts of nasal and salivary glands displayed expression, whereas no expression occurred in the acini. There was decreasing expression along the epithelial linings of the trachea and lower respiratory tract, whereas no expression occurred in the alveoli. The hepatic CYP2A5 inducers pyrazole and phenobarbital neither changed the CYP2A5 expression pattern nor damaged the olfactory mucosa. In contrast, the olfactory toxicants dichlobenil and methimazole induced characteristic changes. The damaged Bowman's glands displayed no expression, whereas the damaged epithelium expressed the enzyme. The CYP2A5 expression pattern is in accordance with previously reported localization of protein and DNA adducts and the toxicity of some CYP2A5 substrates. This suggests that CYP2A5 is an important determinant for the susceptibility of the nasal and respiratory epithelia to protoxicants and procarcinogens.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/14272
url https://fisabio.portalinvestigacion.com/publicaciones/14272
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SAGE PUBLICATIONS LTD
publisher.none.fl_str_mv SAGE PUBLICATIONS LTD
dc.source.none.fl_str_mv JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
ISSN: 00221554
ISSNe: 15515044
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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