Assessment of CRISPR-based genome editing tools for enhancing precision: a computational platform and novel factors for homology-directed repair
Precision and accuracy are paramount for the use of genome editing in clinic, environmental, and industrial applications. These fields require reliable and traceable results to ensure the safety and viability of the resulting genomic modifications. To go in this direction, we have developed a comput...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/688355 |
| Acceso en línea: | http://hdl.handle.net/10803/688355 |
| Access Level: | acceso abierto |
| Palabra clave: | Genome editing bioinformatics PRDM9 homology directed repair CRISPR-Cas systems Edició genòmica bioinformàtica reparació dirigida per homologia 575 |
| Sumario: | Precision and accuracy are paramount for the use of genome editing in clinic, environmental, and industrial applications. These fields require reliable and traceable results to ensure the safety and viability of the resulting genomic modifications. To go in this direction, we have developed a computational platform that can simulate and analyze gene editing outcomes comprehensively and in a versatile manner. Our computational pipeline and web application, CRISPR-Analytics, expands the functionality of currently available tools. In addition, we have proposed the use of a meiotic factor, PRDM9, fused to Cas9 to improve homology directed repair (HDR) efficiency, which is one of the preferred techniques for small edits, but this method often has low efficiency. In conclusion, our computational tools and HDR optimization approach have the potential to facilitate the development of genome editing applications in various fields. These tools could help accelerate progress in the field of genome editing and pave the way for precision genome editing. |
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