Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings

Extrusion bioprinting based on the development of novel bioinks offers the possibility of manufacturing clinically useful tools for wound management. In this study, we show the rheological properties and printability outcomes of two advanced dressings based on platelet-rich plasma (PRP) and platelet...

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Autores: Del Amo, Cristina, Pérez Valle, Arantza, Pérez Garrastachu, Miguel, Jauregui, Ines, Andollo Victoriano, María Noelia, Arluzea de Jauregizar, Jon Andoni, Guerrero Manso, Pedro Manuel, De la Caba Ciriza, María Coro, Andia Ortiz, Isabel María
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/52954
Acceso en línea:http://hdl.handle.net/10810/52954
Access Level:acceso abierto
Palabra clave:cytokines
bioprinting
growth factors
platelet-rich plasma
wound healing
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spelling Plasma-Based Bioinks for Extrusion Bioprinting of Advanced DressingsDel Amo, CristinaPérez Valle, ArantzaPérez Garrastachu, MiguelJauregui, InesAndollo Victoriano, María NoeliaArluzea de Jauregizar, Jon AndoniGuerrero Manso, Pedro ManuelDe la Caba Ciriza, María CoroAndia Ortiz, Isabel Maríacytokinesbioprintinggrowth factorsplatelet-rich plasmawound healingExtrusion bioprinting based on the development of novel bioinks offers the possibility of manufacturing clinically useful tools for wound management. In this study, we show the rheological properties and printability outcomes of two advanced dressings based on platelet-rich plasma (PRP) and platelet-poor plasma (PPP) blended with alginate and loaded with dermal fibroblasts. Measurements taken at 1 h, 4 days, and 18 days showed that both the PRP- and PPP-based dressings retain plasma and platelet proteins, which led to the upregulation of angiogenic and immunomodulatory proteins by embedded fibroblasts (e.g., an up to 69-fold increase in vascular endothelial growth factor (VEGF), an up to 188-fold increase in monocyte chemotactic protein 1 (MCP-1), and an up to 456-fold increase in hepatocyte growth factor (HGF) 18 days after printing). Conditioned media harvested from both PRP and PPP constructs stimulated the proliferation of human umbilical vein endothelial cells (HUVECs), whereas only those from PRP dressings stimulated HUVEC migration, which correlated with the VEGF/MCP-1 and VEGF/HGF ratios. Similarly, the advanced dressings increased the level of interleukin-8 and led to a four-fold change in the level of extracellular matrix protein 1. These findings suggest that careful selection of plasma formulations to fabricate wound dressings can enable regulation of the molecular composition of the microenvironment, as well as paracrine interactions, thereby improving the clinical potential of dressings and providing the possibility to tailor each composition to specific wound types and healing stages.This work was fully supported by a collaborative fundamental research grant from the Basque Government Elkartek program under grant nº. B4H KK-2019-0006-BC.MDPI2021202120212021info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/52954reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/2227-9059/9/8/1023/htminfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/529542026-06-18T09:23:17Z
dc.title.none.fl_str_mv Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
title Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
spellingShingle Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
Del Amo, Cristina
cytokines
bioprinting
growth factors
platelet-rich plasma
wound healing
title_short Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
title_full Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
title_fullStr Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
title_full_unstemmed Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
title_sort Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings
dc.creator.none.fl_str_mv Del Amo, Cristina
Pérez Valle, Arantza
Pérez Garrastachu, Miguel
Jauregui, Ines
Andollo Victoriano, María Noelia
Arluzea de Jauregizar, Jon Andoni
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
Andia Ortiz, Isabel María
author Del Amo, Cristina
author_facet Del Amo, Cristina
Pérez Valle, Arantza
Pérez Garrastachu, Miguel
Jauregui, Ines
Andollo Victoriano, María Noelia
Arluzea de Jauregizar, Jon Andoni
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
Andia Ortiz, Isabel María
author_role author
author2 Pérez Valle, Arantza
Pérez Garrastachu, Miguel
Jauregui, Ines
Andollo Victoriano, María Noelia
Arluzea de Jauregizar, Jon Andoni
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
Andia Ortiz, Isabel María
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cytokines
bioprinting
growth factors
platelet-rich plasma
wound healing
topic cytokines
bioprinting
growth factors
platelet-rich plasma
wound healing
description Extrusion bioprinting based on the development of novel bioinks offers the possibility of manufacturing clinically useful tools for wound management. In this study, we show the rheological properties and printability outcomes of two advanced dressings based on platelet-rich plasma (PRP) and platelet-poor plasma (PPP) blended with alginate and loaded with dermal fibroblasts. Measurements taken at 1 h, 4 days, and 18 days showed that both the PRP- and PPP-based dressings retain plasma and platelet proteins, which led to the upregulation of angiogenic and immunomodulatory proteins by embedded fibroblasts (e.g., an up to 69-fold increase in vascular endothelial growth factor (VEGF), an up to 188-fold increase in monocyte chemotactic protein 1 (MCP-1), and an up to 456-fold increase in hepatocyte growth factor (HGF) 18 days after printing). Conditioned media harvested from both PRP and PPP constructs stimulated the proliferation of human umbilical vein endothelial cells (HUVECs), whereas only those from PRP dressings stimulated HUVEC migration, which correlated with the VEGF/MCP-1 and VEGF/HGF ratios. Similarly, the advanced dressings increased the level of interleukin-8 and led to a four-fold change in the level of extracellular matrix protein 1. These findings suggest that careful selection of plasma formulations to fabricate wound dressings can enable regulation of the molecular composition of the microenvironment, as well as paracrine interactions, thereby improving the clinical potential of dressings and providing the possibility to tailor each composition to specific wound types and healing stages.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/52954
url http://hdl.handle.net/10810/52954
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2227-9059/9/8/1023/htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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