Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

© 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit t...

Descripción completa

Detalles Bibliográficos
Autores: Frey, Caroline F., Regidor Cerrillo, Javier, Marreros, Nelson, García Lunar, Paula, Gutiérrez Expósito, Daniel, Schares, Gereon, Dubey, Jitender P., Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega Mora, Luis Miguel, Álvarez García, Gema
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/19864
Acceso en línea:https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9
https://hdl.handle.net/10612/19864
Access Level:acceso abierto
Palabra clave:Veterinaria
Besnoitia besnoiti
Besnoitia tarandi
Lytic cycle
In vitro model
Isolates
Invasion
Proliferation
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
id ES_8a0a0acdd7effc7a8a560309a72d00a6
oai_identifier_str oai:buleria.unileon.es:10612/19864
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
title Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
spellingShingle Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
Frey, Caroline F.
Veterinaria
Besnoitia besnoiti
Besnoitia tarandi
Lytic cycle
In vitro model
Isolates
Invasion
Proliferation
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
title_short Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
title_full Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
title_fullStr Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
title_full_unstemmed Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
title_sort Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
dc.creator.none.fl_str_mv Frey, Caroline F.
Regidor Cerrillo, Javier
Marreros, Nelson
García Lunar, Paula
Gutiérrez Expósito, Daniel
Schares, Gereon
Dubey, Jitender P.
Gentile, Arcangelo
Jacquiet, Philippe
Shkap, Varda
Cortes, Helder
Ortega Mora, Luis Miguel
Álvarez García, Gema
author Frey, Caroline F.
author_facet Frey, Caroline F.
Regidor Cerrillo, Javier
Marreros, Nelson
García Lunar, Paula
Gutiérrez Expósito, Daniel
Schares, Gereon
Dubey, Jitender P.
Gentile, Arcangelo
Jacquiet, Philippe
Shkap, Varda
Cortes, Helder
Ortega Mora, Luis Miguel
Álvarez García, Gema
author_role author
author2 Regidor Cerrillo, Javier
Marreros, Nelson
García Lunar, Paula
Gutiérrez Expósito, Daniel
Schares, Gereon
Dubey, Jitender P.
Gentile, Arcangelo
Jacquiet, Philippe
Shkap, Varda
Cortes, Helder
Ortega Mora, Luis Miguel
Álvarez García, Gema
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Veterinaria
Besnoitia besnoiti
Besnoitia tarandi
Lytic cycle
In vitro model
Isolates
Invasion
Proliferation
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
topic Veterinaria
Besnoitia besnoiti
Besnoitia tarandi
Lytic cycle
In vitro model
Isolates
Invasion
Proliferation
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
description © 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9
https://hdl.handle.net/10612/19864
url https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9
https://hdl.handle.net/10612/19864
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/AGL2013-46442-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412671439241216
spelling Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolatesFrey, Caroline F.Regidor Cerrillo, JavierMarreros, NelsonGarcía Lunar, PaulaGutiérrez Expósito, DanielSchares, GereonDubey, Jitender P.Gentile, ArcangeloJacquiet, PhilippeShkap, VardaCortes, HelderOrtega Mora, Luis MiguelÁlvarez García, GemaVeterinariaBesnoitia besnoitiBesnoitia tarandiLytic cycleIn vitro modelIsolatesInvasionProliferation3109 Ciencias Veterinarias2401.12 Parasitología Animal© 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.[EN]Background: Bovine besnoitiosis, caused by the protozoan Besnoitia besnoiti, reduces productivity and fertility of affected herds. Besnoitiosis continues to expand in Europe and no effective control tools are currently available. Experimental models are urgently needed. Herein, we describe for the first time the kinetics of standardised in vitro models for the B. besnoiti lytic cycle. This will aid to study the pathogenesis of the disease, in the screening for vaccine targets and drugs potentially useful for the treatment of besnoitiosis. Methods: We compared invasion and proliferation of one B. tarandi (from Finland) and seven B. besnoiti isolates (Bb-Spain1, Bb-Spain2, Bb-Israel, Bb-Evora03, Bb-Ger1, Bb-France, Bb-Italy2) in MARC-145 cell culture. Host cell invasion was studied at 4, 6, 8 and 24 h post infection (hpi), and proliferation characteristics were compared at 24, 48, 72, 96, 120, and 144 hpi. Results: In Besnoitia spp., the key parameters that determine the sequential adhesion-invasion, proliferation and egress steps are clearly distinct from those in the related apicomplexans Toxoplasma gondii and Neospora caninum. Besnoitia spp. host cell invasion is a rather slow process, since only 50 % of parasites were found intracellular after 3-6 h of exposure to host cells, and invasion still took place after 24 h. Invasion efficacy was significantly higher for Bb-France, Bb-Evora03 and Bb-Israel. In addition, the time span for endodyogeny to take place was as long as 18-35 h. Bb-Israel and B. tarandi isolates were most prolific, as determined by the tachyzoite yield at 72 hpi. The total tachyzoite yield could not be predicted neither by invasion-related parameters (velocity and half time invasion) nor by proliferation parameters (lag phase and doubling time (dT)). The lytic cycle of Besnoitia was asynchronous as evidenced by the presence of three different plaque-forming tachyzoite categories (lysis plaques, large and small parasitophorous vacuoles). Conclusions: This study provides first insights into the lytic cycle of B. besnoiti isolates and a standardised in vitro model that allows screening of drug candidates for the treatment of besnoitiosis.SIThis study was funded by the Spanish Ministry of Economy and Competitiveness (AGL2013-46442-R) and CYTED (Thematic Network 113RT0469 Protozoovac). CFF and NM are supported by the Swiss National Science Foundation (grants no. PBBEP3_141435 and PBBEP3_139398, respectively). We gratefully acknowledge Vanessa Navarro for her excellent technical assistance, and Andrew Hemphill for critical reading of the manuscript.BMCSanidad AnimalFacultad de Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9https://hdl.handle.net/10612/19864reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/AGL2013-46442-Rhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/198642026-06-24T12:43:27Z
score 15,300724

Similares en LA Referencia