Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients

Profiling of circulating immune cells provides valuable insight to the pathophysiology of acute rejection in organ transplantation. Herein we characterized the peripheral blood mononuclear cells in simultaneous kidney-pancreas transplant recipients. We conducted a retrospective analysis in a biopsy-...

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Autores: Rovira Juárez, Jordi, Ramírez Bajo, María José, Bañón Maneus, Elisenda, Hierro García, Natalia, Lazo Rodríguez, Marta, Piñeiro, Gastón Julio, Montagud Marrahi, Enrique, Cucchiari, David, Revuelta, Ignacio, Cuatrecasas Freixas, Miriam, Campistol Plana, Josep M., Ricart Brulles, Ma. José, Diekmann, Fritz, García Criado, Ángeles, Ventura Aguiar, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/206231
Acceso en línea:https://hdl.handle.net/2445/206231
Access Level:acceso abierto
Palabra clave:Trasplantament d'òrgans
Ronyó
Pàncrees
Transplantation of organs
Kidney
Pancreas
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spelling Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant RecipientsRovira Juárez, JordiRamírez Bajo, María JoséBañón Maneus, ElisendaHierro García, NataliaLazo Rodríguez, MartaPiñeiro, Gastón JulioMontagud Marrahi, EnriqueCucchiari, DavidRevuelta, IgnacioCuatrecasas Freixas, MiriamCampistol Plana, Josep M.Ricart Brulles, Ma. JoséDiekmann, FritzGarcía Criado, ÁngelesVentura Aguiar, PedroTrasplantament d'òrgansRonyóPàncreesTransplantation of organsKidneyPancreasProfiling of circulating immune cells provides valuable insight to the pathophysiology of acute rejection in organ transplantation. Herein we characterized the peripheral blood mononuclear cells in simultaneous kidney-pancreas transplant recipients. We conducted a retrospective analysis in a biopsy-matched cohort (n = 67) and compared patients with biopsy proven acute rejection (BPAR; 41%) to those without rejection (No-AR). We observed that CD3+ T cells, both CD8+ and CD4+, as well as CD19+ B cells were increased in patients with BPAR, particularly in biopsies performed in the early post-transplant period (<3 months). During this period immune subsets presented a good discriminative ability (CD4+ AUC 0.79; CD8+ AUC 0.80; B cells AUC 0.86; p < 0.05) and outperformed lipase (AUC 0.62; p = 0.12) for the diagnosis of acute rejection. We further evaluated whether this could be explained by differences in frequencies prior to transplantation. Patients presenting with early post-transplant rejection (<3 months) had a significant increase in T-cell frequencies pre-transplant, both CD4+ T cells and CD8+ T cells (p < 0.01), which were associated with a significant inferior rejection-free graft survival. T cell frequencies in peripheral blood correlated with pancreas acute rejection episodes, and variations prior to transplantation were associated with pancreas early acute rejection.Copyright © 2022 Rovira, Ramirez-Bajo, Bañón-Maneus, Hierro-Garcia, Lazo-Rodriguez, Piñeiro, Montagud-Marrahi, Cucchiari, Revuelta, Cuatrecasas, Campistol, Ricart, Diekmann, Garcia-Criado and Ventura-Aguiar.Frontiers2024202420232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/206231Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/ti.2022.10639Transplant International, 2022, vol. 35https://doi.org/10.3389/ti.2022.10639cc by (c) Rovira, Jordi et al, 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2062312026-05-29T05:05:01Z
dc.title.none.fl_str_mv Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
title Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
spellingShingle Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
Rovira Juárez, Jordi
Trasplantament d'òrgans
Ronyó
Pàncrees
Transplantation of organs
Kidney
Pancreas
title_short Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
title_full Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
title_fullStr Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
title_full_unstemmed Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
title_sort Immune Profiling of Peripheral Blood Mononuclear Cells at Pancreas Acute Rejection Episodes in Kidney-Pancreas Transplant Recipients
dc.creator.none.fl_str_mv Rovira Juárez, Jordi
Ramírez Bajo, María José
Bañón Maneus, Elisenda
Hierro García, Natalia
Lazo Rodríguez, Marta
Piñeiro, Gastón Julio
Montagud Marrahi, Enrique
Cucchiari, David
Revuelta, Ignacio
Cuatrecasas Freixas, Miriam
Campistol Plana, Josep M.
Ricart Brulles, Ma. José
Diekmann, Fritz
García Criado, Ángeles
Ventura Aguiar, Pedro
author Rovira Juárez, Jordi
author_facet Rovira Juárez, Jordi
Ramírez Bajo, María José
Bañón Maneus, Elisenda
Hierro García, Natalia
Lazo Rodríguez, Marta
Piñeiro, Gastón Julio
Montagud Marrahi, Enrique
Cucchiari, David
Revuelta, Ignacio
Cuatrecasas Freixas, Miriam
Campistol Plana, Josep M.
Ricart Brulles, Ma. José
Diekmann, Fritz
García Criado, Ángeles
Ventura Aguiar, Pedro
author_role author
author2 Ramírez Bajo, María José
Bañón Maneus, Elisenda
Hierro García, Natalia
Lazo Rodríguez, Marta
Piñeiro, Gastón Julio
Montagud Marrahi, Enrique
Cucchiari, David
Revuelta, Ignacio
Cuatrecasas Freixas, Miriam
Campistol Plana, Josep M.
Ricart Brulles, Ma. José
Diekmann, Fritz
García Criado, Ángeles
Ventura Aguiar, Pedro
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trasplantament d'òrgans
Ronyó
Pàncrees
Transplantation of organs
Kidney
Pancreas
topic Trasplantament d'òrgans
Ronyó
Pàncrees
Transplantation of organs
Kidney
Pancreas
description Profiling of circulating immune cells provides valuable insight to the pathophysiology of acute rejection in organ transplantation. Herein we characterized the peripheral blood mononuclear cells in simultaneous kidney-pancreas transplant recipients. We conducted a retrospective analysis in a biopsy-matched cohort (n = 67) and compared patients with biopsy proven acute rejection (BPAR; 41%) to those without rejection (No-AR). We observed that CD3+ T cells, both CD8+ and CD4+, as well as CD19+ B cells were increased in patients with BPAR, particularly in biopsies performed in the early post-transplant period (<3 months). During this period immune subsets presented a good discriminative ability (CD4+ AUC 0.79; CD8+ AUC 0.80; B cells AUC 0.86; p < 0.05) and outperformed lipase (AUC 0.62; p = 0.12) for the diagnosis of acute rejection. We further evaluated whether this could be explained by differences in frequencies prior to transplantation. Patients presenting with early post-transplant rejection (<3 months) had a significant increase in T-cell frequencies pre-transplant, both CD4+ T cells and CD8+ T cells (p < 0.01), which were associated with a significant inferior rejection-free graft survival. T cell frequencies in peripheral blood correlated with pancreas acute rejection episodes, and variations prior to transplantation were associated with pancreas early acute rejection.Copyright © 2022 Rovira, Ramirez-Bajo, Bañón-Maneus, Hierro-Garcia, Lazo-Rodriguez, Piñeiro, Montagud-Marrahi, Cucchiari, Revuelta, Cuatrecasas, Campistol, Ricart, Diekmann, Garcia-Criado and Ventura-Aguiar.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/206231
url https://hdl.handle.net/2445/206231
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/ti.2022.10639
Transplant International, 2022, vol. 35
https://doi.org/10.3389/ti.2022.10639
dc.rights.none.fl_str_mv cc by (c) Rovira, Jordi et al, 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Rovira, Jordi et al, 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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