Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes

Paclitaxel (PTX), a widely used anticancer agent, is found in the inner bark of several Taxus species, although at such low levels that its extraction is ecologically unsustainable. Biotechnological platforms based on Taxus sp. cell cultures offer an eco-friendlier approach to PTX production, with y...

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Autores: Escrich, Ainoa, Almagro, Lorena, Moyano Claramunt, Elisabet, Cusidó Vidal, Rosa M., Bonfill Baldrich, Ma. Mercedes, Hosseini, Bahman, Palazón Barandela, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/184312
Acceso en línea:https://hdl.handle.net/2445/184312
Access Level:acceso abierto
Palabra clave:Cèl·lules i teixits vegetals
Cultiu de cèl·lules i teixits vegetals
Compostos bioactius vegetals
Plant cells and tissues
Plant cell and tissue culture
Plant bioactive compounds
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spelling Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenesEscrich, AinoaAlmagro, LorenaMoyano Claramunt, ElisabetCusidó Vidal, Rosa M.Bonfill Baldrich, Ma. MercedesHosseini, BahmanPalazón Barandela, JavierCèl·lules i teixits vegetalsCultiu de cèl·lules i teixits vegetalsCompostos bioactius vegetalsPlant cells and tissuesPlant cell and tissue culturePlant bioactive compoundsPaclitaxel (PTX), a widely used anticancer agent, is found in the inner bark of several Taxus species, although at such low levels that its extraction is ecologically unsustainable. Biotechnological platforms based on Taxus sp. cell cultures offer an eco-friendlier approach to PTX production, with yields that can be improved by elicitation. However, the also limited excretion of target compounds from the producer cells to the medium hampers their extraction and purification. In this context, we studied the effect of treating T. media cell cultures with the elicitor coronatine (COR) and calix[8]arenes (CAL), nanoparticles that can host lipophilic compounds within their macrocyclic scaffold. The highest taxane production (103.5 mg.L− 1 ), achieved after treatment with COR (1 μM) and CAL (10 mg.L− 1 ), was 15-fold greater than in the control, and PTX represented 82% of the total taxanes analyzed. Expression levels of the flux-limiting PTX biosynthetic genes, BAPT and DBTNBT, increased after the addition of COR, confirming its elicitor action, but not CAL. The CAL treatment significantly enhanced taxane excretion, especially when production levels were increased by COR; 98% of the total taxanes were found in the culture medium after COR + CAL treatment. By forming complexes with PTX, the nanoparticles facilitated its excretion to the medium, and by protecting cells from PTX toxicity, its intra-and extra-cellular degradation may have been avoided. The addition of COR and CAL to T. media cell cultures is therefore a bio-sustainable and economically viable system to improve the yield of this important anticancer compound.Elsevier Masson SAS2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/184312Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.plaphy.2021.03.047Plant Physiology and Biochemistry, 2021, vol. 163, p. 68-75https://doi.org/10.1016/j.plaphy.2021.03.047cc by (c) Ainoa Escrich, et al. , 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1843122026-05-29T05:05:01Z
dc.title.none.fl_str_mv Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
title Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
spellingShingle Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
Escrich, Ainoa
Cèl·lules i teixits vegetals
Cultiu de cèl·lules i teixits vegetals
Compostos bioactius vegetals
Plant cells and tissues
Plant cell and tissue culture
Plant bioactive compounds
title_short Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
title_full Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
title_fullStr Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
title_full_unstemmed Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
title_sort Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
dc.creator.none.fl_str_mv Escrich, Ainoa
Almagro, Lorena
Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Bonfill Baldrich, Ma. Mercedes
Hosseini, Bahman
Palazón Barandela, Javier
author Escrich, Ainoa
author_facet Escrich, Ainoa
Almagro, Lorena
Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Bonfill Baldrich, Ma. Mercedes
Hosseini, Bahman
Palazón Barandela, Javier
author_role author
author2 Almagro, Lorena
Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Bonfill Baldrich, Ma. Mercedes
Hosseini, Bahman
Palazón Barandela, Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules i teixits vegetals
Cultiu de cèl·lules i teixits vegetals
Compostos bioactius vegetals
Plant cells and tissues
Plant cell and tissue culture
Plant bioactive compounds
topic Cèl·lules i teixits vegetals
Cultiu de cèl·lules i teixits vegetals
Compostos bioactius vegetals
Plant cells and tissues
Plant cell and tissue culture
Plant bioactive compounds
description Paclitaxel (PTX), a widely used anticancer agent, is found in the inner bark of several Taxus species, although at such low levels that its extraction is ecologically unsustainable. Biotechnological platforms based on Taxus sp. cell cultures offer an eco-friendlier approach to PTX production, with yields that can be improved by elicitation. However, the also limited excretion of target compounds from the producer cells to the medium hampers their extraction and purification. In this context, we studied the effect of treating T. media cell cultures with the elicitor coronatine (COR) and calix[8]arenes (CAL), nanoparticles that can host lipophilic compounds within their macrocyclic scaffold. The highest taxane production (103.5 mg.L− 1 ), achieved after treatment with COR (1 μM) and CAL (10 mg.L− 1 ), was 15-fold greater than in the control, and PTX represented 82% of the total taxanes analyzed. Expression levels of the flux-limiting PTX biosynthetic genes, BAPT and DBTNBT, increased after the addition of COR, confirming its elicitor action, but not CAL. The CAL treatment significantly enhanced taxane excretion, especially when production levels were increased by COR; 98% of the total taxanes were found in the culture medium after COR + CAL treatment. By forming complexes with PTX, the nanoparticles facilitated its excretion to the medium, and by protecting cells from PTX toxicity, its intra-and extra-cellular degradation may have been avoided. The addition of COR and CAL to T. media cell cultures is therefore a bio-sustainable and economically viable system to improve the yield of this important anticancer compound.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184312
url https://hdl.handle.net/2445/184312
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.plaphy.2021.03.047
Plant Physiology and Biochemistry, 2021, vol. 163, p. 68-75
https://doi.org/10.1016/j.plaphy.2021.03.047
dc.rights.none.fl_str_mv cc by (c) Ainoa Escrich, et al. , 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Ainoa Escrich, et al. , 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson SAS
publisher.none.fl_str_mv Elsevier Masson SAS
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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