A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen

Pseudomonas aeruginosa is an important opportunistic human pathogen with high prevalence in nosocomial infections. This microorganism is a good model for understanding biological processes such as the quorum-sensing response, the metabolic integration of virulence, the mechanisms of global regulatio...

Full description

Bibliographic Details
Authors: Reales Calderón, José Antonio, Sun, Z., Mascaraque, Victoria, Pérez Navarro, E., Vialás, Vital, Deutsch, Eric W., Moritz, Robert L., Gil , Concha, Martínez, J.L., Molero Martín-Portugués, María Gloria
Format: article
Publication Date:2021
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/6804
Online Access:https://hdl.handle.net/20.500.14352/6804
Access Level:Open access
Keyword:579
576.8
Pseudomonas
Bacterial PeptideAtlas
Pathogen
Database
Proteomics
Tool for targeted proteomic studies
Farmacia
Microbiología (Farmacia)
Parasitología (Farmacia)
3302.03 Microbiología Industrial
id ES_89e86a6d33e8e26cd0c2baba7eef6489
oai_identifier_str oai:docta.ucm.es:20.500.14352/6804
network_acronym_str ES
network_name_str España
repository_id_str
spelling A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogenReales Calderón, José AntonioSun, Z.Mascaraque, VictoriaPérez Navarro, E.Vialás, VitalDeutsch, Eric W.Moritz, Robert L.Gil , ConchaMartínez, J.L.Molero Martín-Portugués, María Gloria579576.8PseudomonasBacterial PeptideAtlasPathogenDatabaseProteomicsTool for targeted proteomic studiesFarmaciaMicrobiología (Farmacia)Parasitología (Farmacia)3302.03 Microbiología IndustrialPseudomonas aeruginosa is an important opportunistic human pathogen with high prevalence in nosocomial infections. This microorganism is a good model for understanding biological processes such as the quorum-sensing response, the metabolic integration of virulence, the mechanisms of global regulation of bacterial physiology, and the evolution of antibiotic resistance. Till now, P. aeruginosa proteomic data, although available in several on-line repositories, were dispersed and difficult to access. In the present work, proteomes of the PAO1 strain grown under different conditions and from diverse cellular compartments have been joined to build the Pseudomonas PeptideAtlas. This resource is a comprehensive mass spectrometry-derived peptide and inferred protein database with 71.3% coverage of the total predicted proteome of P. aeruginosa PAO1, the highest coverage among bacterial PeptideAtlas datasets. The proteins included cover 89% of metabolic proteins, 72% of proteins involved in genetic information processing, 83% of proteins responsible for environmental information processing, more than 88% of the ones related to quorum sensing and biofilm formation, and 89% of proteins responsible for antimicrobial resistance. It exemplifies a necessary tool for targeted proteomics studies, system-wide observations, and cross-species observational studies. The manuscript describes the building of the PeptideAtlas and the contribution of the different proteomic data used. Significance: Pseudomonas aeruginosa is among the most versatile human bacterial pathogens. Studies of its proteome are very important as they can reveal virulence factors and mechanisms of antibiotic resistance. The construction of a proteomic resource such as the PeptideAtlas enables targeted proteomics studies, system-wide observations, and cross-species observational studies.ElsevierUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/6804reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/68042026-06-02T12:44:21Z
dc.title.none.fl_str_mv A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
title A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
spellingShingle A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
Reales Calderón, José Antonio
579
576.8
Pseudomonas
Bacterial PeptideAtlas
Pathogen
Database
Proteomics
Tool for targeted proteomic studies
Farmacia
Microbiología (Farmacia)
Parasitología (Farmacia)
3302.03 Microbiología Industrial
title_short A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
title_full A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
title_fullStr A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
title_full_unstemmed A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
title_sort A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
dc.creator.none.fl_str_mv Reales Calderón, José Antonio
Sun, Z.
Mascaraque, Victoria
Pérez Navarro, E.
Vialás, Vital
Deutsch, Eric W.
Moritz, Robert L.
Gil , Concha
Martínez, J.L.
Molero Martín-Portugués, María Gloria
author Reales Calderón, José Antonio
author_facet Reales Calderón, José Antonio
Sun, Z.
Mascaraque, Victoria
Pérez Navarro, E.
Vialás, Vital
Deutsch, Eric W.
Moritz, Robert L.
Gil , Concha
Martínez, J.L.
Molero Martín-Portugués, María Gloria
author_role author
author2 Sun, Z.
Mascaraque, Victoria
Pérez Navarro, E.
Vialás, Vital
Deutsch, Eric W.
Moritz, Robert L.
Gil , Concha
Martínez, J.L.
Molero Martín-Portugués, María Gloria
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
576.8
Pseudomonas
Bacterial PeptideAtlas
Pathogen
Database
Proteomics
Tool for targeted proteomic studies
Farmacia
Microbiología (Farmacia)
Parasitología (Farmacia)
3302.03 Microbiología Industrial
topic 579
576.8
Pseudomonas
Bacterial PeptideAtlas
Pathogen
Database
Proteomics
Tool for targeted proteomic studies
Farmacia
Microbiología (Farmacia)
Parasitología (Farmacia)
3302.03 Microbiología Industrial
description Pseudomonas aeruginosa is an important opportunistic human pathogen with high prevalence in nosocomial infections. This microorganism is a good model for understanding biological processes such as the quorum-sensing response, the metabolic integration of virulence, the mechanisms of global regulation of bacterial physiology, and the evolution of antibiotic resistance. Till now, P. aeruginosa proteomic data, although available in several on-line repositories, were dispersed and difficult to access. In the present work, proteomes of the PAO1 strain grown under different conditions and from diverse cellular compartments have been joined to build the Pseudomonas PeptideAtlas. This resource is a comprehensive mass spectrometry-derived peptide and inferred protein database with 71.3% coverage of the total predicted proteome of P. aeruginosa PAO1, the highest coverage among bacterial PeptideAtlas datasets. The proteins included cover 89% of metabolic proteins, 72% of proteins involved in genetic information processing, 83% of proteins responsible for environmental information processing, more than 88% of the ones related to quorum sensing and biofilm formation, and 89% of proteins responsible for antimicrobial resistance. It exemplifies a necessary tool for targeted proteomics studies, system-wide observations, and cross-species observational studies. The manuscript describes the building of the PeptideAtlas and the contribution of the different proteomic data used. Significance: Pseudomonas aeruginosa is among the most versatile human bacterial pathogens. Studies of its proteome are very important as they can reveal virulence factors and mechanisms of antibiotic resistance. The construction of a proteomic resource such as the PeptideAtlas enables targeted proteomics studies, system-wide observations, and cross-species observational studies.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/6804
url https://hdl.handle.net/20.500.14352/6804
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412662075457537
score 15.300719

Similar Items