Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms

[EN] Glaesserella (Haemophilus) parasuis, the causative agent of Glässer’s disease, is present in most pig farms as an early colonizer of the upper respiratory tract. It exhibits remarkable variability in virulence and antimicrobial resistance (AMR), with virulent strains capable of inducing respira...

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Detalhes bibliográficos
Autores: González Fernández, Alba, Mencía Ares, Óscar, García Iglesias, María José, Petrocchi Rilo, Máximo, Miguélez Pérez, Rubén, Gutiérrez Martín, César Bernardo, Martínez Martínez, Sonia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24438
Acesso em linha:https://www.mdpi.com/2079-6382/13/8/741
https://hdl.handle.net/10612/24438
Access Level:acceso abierto
Palavra-chave:Sanidad animal
Veterinaria
Antimicrobial susceptibility profiling
Glaesserella parasuis
Haemophilus parasuis
Pathotyping
Pig
Porcine respiratory disease complex
TbpB clustering
VtaA genes
3109 Ciencias Veterinarias
2414.01 Antibióticos
3109.05 Microbiología
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oai_identifier_str oai:buleria.unileon.es:10612/24438
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repository_id_str
spelling Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine FarmsGonzález Fernández, AlbaMencía Ares, ÓscarGarcía Iglesias, María JoséPetrocchi Rilo, MáximoMiguélez Pérez, RubénGutiérrez Martín, César BernardoMartínez Martínez, SoniaSanidad animalVeterinariaAntimicrobial susceptibility profilingGlaesserella parasuisHaemophilus parasuisPathotypingPigPorcine respiratory disease complexTbpB clusteringVtaA genes3109 Ciencias Veterinarias2414.01 Antibióticos3109.05 Microbiología[EN] Glaesserella (Haemophilus) parasuis, the causative agent of Glässer’s disease, is present in most pig farms as an early colonizer of the upper respiratory tract. It exhibits remarkable variability in virulence and antimicrobial resistance (AMR), with virulent strains capable of inducing respiratory or systemic disease. This study aimed to characterize the virulence and the AMR profiles in 65 G. parasuis isolates recovered from Spanish swine farms. Virulence was assessed using multiplex leader sequence (LS)-PCR targeting vtaA genes, with all isolates identified as clinical (presumed virulent). Pathotyping based on ten pangenome genes revealed the virulent HPS_22970 as the most frequent (83.1%). Diverse pathotype profiles were observed, with 29 unique gene combinations and two isolates carrying only potentially non-virulent pangenome genes. AMR phenotyping showed widespread resistance, with 63.3% classified as multidrug resistant, and high resistance to clindamycin (98.3%) and tylosin (93.3%). A very strong association was found between certain pathotype genes and AMR phenotypes, notably between the virulent HPS_22970 and tetracycline resistance (p < 0.001; Φ = 0.58). This study reveals the wide diversity and complexity of G. parasuis pathogenicity and AMR phenotype, emphasizing the need for the targeted characterization of clinical isolates to ensure appropriate antimicrobial treatments and the implementation of prophylactic measures against virulent strains.SIThis publication is part of the I+D+I project PID2019-105125RB-I00, funded by MCIN/AEI/ 10.13039/501100011033 (Agencia Estatal de Investigación, Ministerio de Ciencia e Innovación, Spanish Government). Alba González-Fernández holds a grant from the University of León. Rubén Miguélez-Pérez holds a grant from Junta de Castilla y León co-financed by the European Social FundMDPISanidad AnimalFacultad de Veterinaria2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/2079-6382/13/8/741https://hdl.handle.net/10612/24438reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-105125RB-I00http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/244382026-06-24T12:43:27Z
dc.title.none.fl_str_mv Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
title Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
spellingShingle Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
González Fernández, Alba
Sanidad animal
Veterinaria
Antimicrobial susceptibility profiling
Glaesserella parasuis
Haemophilus parasuis
Pathotyping
Pig
Porcine respiratory disease complex
TbpB clustering
VtaA genes
3109 Ciencias Veterinarias
2414.01 Antibióticos
3109.05 Microbiología
title_short Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
title_full Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
title_fullStr Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
title_full_unstemmed Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
title_sort Virulence and Antimicrobial Resistance Characterization of Glaesserella parasuis Isolates Recovered from Spanish Swine Farms
dc.creator.none.fl_str_mv González Fernández, Alba
Mencía Ares, Óscar
García Iglesias, María José
Petrocchi Rilo, Máximo
Miguélez Pérez, Rubén
Gutiérrez Martín, César Bernardo
Martínez Martínez, Sonia
author González Fernández, Alba
author_facet González Fernández, Alba
Mencía Ares, Óscar
García Iglesias, María José
Petrocchi Rilo, Máximo
Miguélez Pérez, Rubén
Gutiérrez Martín, César Bernardo
Martínez Martínez, Sonia
author_role author
author2 Mencía Ares, Óscar
García Iglesias, María José
Petrocchi Rilo, Máximo
Miguélez Pérez, Rubén
Gutiérrez Martín, César Bernardo
Martínez Martínez, Sonia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Veterinaria
Antimicrobial susceptibility profiling
Glaesserella parasuis
Haemophilus parasuis
Pathotyping
Pig
Porcine respiratory disease complex
TbpB clustering
VtaA genes
3109 Ciencias Veterinarias
2414.01 Antibióticos
3109.05 Microbiología
topic Sanidad animal
Veterinaria
Antimicrobial susceptibility profiling
Glaesserella parasuis
Haemophilus parasuis
Pathotyping
Pig
Porcine respiratory disease complex
TbpB clustering
VtaA genes
3109 Ciencias Veterinarias
2414.01 Antibióticos
3109.05 Microbiología
description [EN] Glaesserella (Haemophilus) parasuis, the causative agent of Glässer’s disease, is present in most pig farms as an early colonizer of the upper respiratory tract. It exhibits remarkable variability in virulence and antimicrobial resistance (AMR), with virulent strains capable of inducing respiratory or systemic disease. This study aimed to characterize the virulence and the AMR profiles in 65 G. parasuis isolates recovered from Spanish swine farms. Virulence was assessed using multiplex leader sequence (LS)-PCR targeting vtaA genes, with all isolates identified as clinical (presumed virulent). Pathotyping based on ten pangenome genes revealed the virulent HPS_22970 as the most frequent (83.1%). Diverse pathotype profiles were observed, with 29 unique gene combinations and two isolates carrying only potentially non-virulent pangenome genes. AMR phenotyping showed widespread resistance, with 63.3% classified as multidrug resistant, and high resistance to clindamycin (98.3%) and tylosin (93.3%). A very strong association was found between certain pathotype genes and AMR phenotypes, notably between the virulent HPS_22970 and tetracycline resistance (p < 0.001; Φ = 0.58). This study reveals the wide diversity and complexity of G. parasuis pathogenicity and AMR phenotype, emphasizing the need for the targeted characterization of clinical isolates to ensure appropriate antimicrobial treatments and the implementation of prophylactic measures against virulent strains.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2079-6382/13/8/741
https://hdl.handle.net/10612/24438
url https://www.mdpi.com/2079-6382/13/8/741
https://hdl.handle.net/10612/24438
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-105125RB-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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