Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers

[ES] Key message We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Abstract Somaclonal variation is a general consequence of the tissue c...

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Detalles Bibliográficos
Autores: Coronel Ramones, Carlos Javier, González Cordero, Ana Isabel, Ruiz Sánchez, María Luisa, Polanco de la Puente, Carlos Gaspar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/27584
Acceso en línea:https://link-springer-com.usal.idm.oclc.org/article/10.1007/s00299-017-2217-x
https://hdl.handle.net/10612/27584
Access Level:acceso abierto
Palabra clave:Biología
Botánica
Genética
Somaclonal variation
Epigenetic instability
Methylation
MetAFLP
TMD markers
2409.92 Genética Molecular de Plantas
2417 Biología Vegetal (Botánica)
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oai_identifier_str oai:buleria.unileon.es:10612/27584
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markersCoronel Ramones, Carlos JavierGonzález Cordero, Ana IsabelRuiz Sánchez, María LuisaPolanco de la Puente, Carlos GasparBiologíaBotánicaGenéticaSomaclonal variationEpigenetic instabilityMethylationMetAFLPTMD markers2409.92 Genética Molecular de Plantas2417 Biología Vegetal (Botánica)[ES] Key message We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Abstract Somaclonal variation is a general consequence of the tissue culture process that has to be analyzed specifically when regenerated plants are obtained in any plant species. Currently, there are few studies about the variability comprising sequence changes and methylation status at the DNA level, generated by the culture of A. thaliana cells and tissues. In this work, two types of highly reproducible molecular markers, modified methylation sensitive AFLP (metAFLP) and transposon methylation display (TMD) have been used for the first time in this species to analyze the nucleotide and cytosine methylation changes induced by transformation and tissue culture protocols. We found significantly higher average methylation values (7.5%) in regenerated and transgenic plants when compared to values obtained from seed derived plants (3.2%) and that the main component of the somaclonal variation present in Arabidopsis clonal plants is genetic rather than epigenetic. However, we have found that the Arabidopsis regenerated and transgenic plants had a higher number of non-fully methylated sites flanking transposable elements than the control plants, and therefore, their mobilization can be facilitated. These data provide further evidence that changes in nucleotide sequence and methylation status occur in the Arabidopsis genome during in vitro tissue culture frequently enough to be an important source of variation in this speciesSIThis work was supported by the Spanish DGICYT grant AGL2066-14249-C02-02 and by the Junta de Castilla y León grant LE052A06SspringerGeneticaFacultad de Ciencias Biologicas y Ambientales2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://link-springer-com.usal.idm.oclc.org/article/10.1007/s00299-017-2217-xhttps://hdl.handle.net/10612/27584reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MEC//AGL2006-14249-C02-02info:eu-repo/grantAgreement/Junta de Castilla y León//LE052A06info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/275842026-06-24T12:43:27Z
dc.title.none.fl_str_mv Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
title Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
spellingShingle Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
Coronel Ramones, Carlos Javier
Biología
Botánica
Genética
Somaclonal variation
Epigenetic instability
Methylation
MetAFLP
TMD markers
2409.92 Genética Molecular de Plantas
2417 Biología Vegetal (Botánica)
title_short Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
title_full Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
title_fullStr Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
title_full_unstemmed Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
title_sort Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers
dc.creator.none.fl_str_mv Coronel Ramones, Carlos Javier
González Cordero, Ana Isabel
Ruiz Sánchez, María Luisa
Polanco de la Puente, Carlos Gaspar
author Coronel Ramones, Carlos Javier
author_facet Coronel Ramones, Carlos Javier
González Cordero, Ana Isabel
Ruiz Sánchez, María Luisa
Polanco de la Puente, Carlos Gaspar
author_role author
author2 González Cordero, Ana Isabel
Ruiz Sánchez, María Luisa
Polanco de la Puente, Carlos Gaspar
author2_role author
author
author
dc.contributor.none.fl_str_mv Genetica
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Botánica
Genética
Somaclonal variation
Epigenetic instability
Methylation
MetAFLP
TMD markers
2409.92 Genética Molecular de Plantas
2417 Biología Vegetal (Botánica)
topic Biología
Botánica
Genética
Somaclonal variation
Epigenetic instability
Methylation
MetAFLP
TMD markers
2409.92 Genética Molecular de Plantas
2417 Biología Vegetal (Botánica)
description [ES] Key message We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Abstract Somaclonal variation is a general consequence of the tissue culture process that has to be analyzed specifically when regenerated plants are obtained in any plant species. Currently, there are few studies about the variability comprising sequence changes and methylation status at the DNA level, generated by the culture of A. thaliana cells and tissues. In this work, two types of highly reproducible molecular markers, modified methylation sensitive AFLP (metAFLP) and transposon methylation display (TMD) have been used for the first time in this species to analyze the nucleotide and cytosine methylation changes induced by transformation and tissue culture protocols. We found significantly higher average methylation values (7.5%) in regenerated and transgenic plants when compared to values obtained from seed derived plants (3.2%) and that the main component of the somaclonal variation present in Arabidopsis clonal plants is genetic rather than epigenetic. However, we have found that the Arabidopsis regenerated and transgenic plants had a higher number of non-fully methylated sites flanking transposable elements than the control plants, and therefore, their mobilization can be facilitated. These data provide further evidence that changes in nucleotide sequence and methylation status occur in the Arabidopsis genome during in vitro tissue culture frequently enough to be an important source of variation in this species
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://link-springer-com.usal.idm.oclc.org/article/10.1007/s00299-017-2217-x
https://hdl.handle.net/10612/27584
url https://link-springer-com.usal.idm.oclc.org/article/10.1007/s00299-017-2217-x
https://hdl.handle.net/10612/27584
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MEC//AGL2006-14249-C02-02
info:eu-repo/grantAgreement/Junta de Castilla y León//LE052A06
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sspringer
publisher.none.fl_str_mv Sspringer
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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