Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients

17 p.

Detalhes bibliográficos
Autores: Monserrat Sanz, Jorge|||0000-0003-1775-4645, Álvarez De Mon Soto, Melchor|||0000-0003-1309-7510, Bohorquez Heras, Cristina|||0000-0002-1601-2079, Gómez Lahoz, Ana María|||0000-0003-3981-5138, Movasat Hajkhan, Atusa, Pérez Gómez, Ana María, Ruiz Sanjuán, Lucía, Díaz Martín, David|||0000-0002-5672-6658, Sánchez Atrio, Ana Isabel, Albarrán Hernández, Fernando, Sanz Paz, Ignacio
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universidad de Alcalá (UAH)
Repositório:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglês
OAI Identifier:oai:ebuah.uah.es:10017/63939
Acesso em linha:http://hdl.handle.net/10017/63939
https://dx.doi.org/10.3390/ijms21186847
Access Level:Acceso aberto
Palavra-chave:Naïve rheumatoid arthritis
CD4+ T-lymphocytes
Methotrexate response
IFNgamma, IL-17A
STAT expression
Medicina
Medicine
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spelling Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis PatientsMonserrat Sanz, Jorge|||0000-0003-1775-4645Álvarez De Mon Soto, Melchor|||0000-0003-1309-7510Bohorquez Heras, Cristina|||0000-0002-1601-2079Gómez Lahoz, Ana María|||0000-0003-3981-5138Movasat Hajkhan, AtusaPérez Gómez, Ana MaríaRuiz Sanjuán, LucíaDíaz Martín, David|||0000-0002-5672-6658Sánchez Atrio, Ana IsabelAlbarrán Hernández, FernandoSanz Paz, IgnacioNaïve rheumatoid arthritisCD4+ T-lymphocytesMethotrexate responseIFNgamma, IL-17ASTAT expressionMedicinaMedicine17 p.CD4+T-lymphocytes are relevant in the pathogenesis of rheumatoid arthritis (RA), however, their potential involvement in early RA remains elusive. Methotrexate (MTX) is a commonly used disease-modifying antirheumatic drug (DMARD), but its mechanism has not been fully established. In 47 new-onset DMARD-naïve RA patients, we investigated the pattern of IFN?, IL-4 and IL-17A expression by naïve (TN), central (TCM), effector memory (TEM) and effector (TE) CD4+ subsets; their STAT-1, STAT-6 and STAT-3 transcription factors phosphorylation, and the circulating levels of IFN?, IL-4 and IL-17. We also studied the RA patients after 3 and 6 months of MTX treatment and according their clinical response. CD4+T-lymphocyte subsets and cytokine expression were measured using flow cytometry. New-onset DMARD-naïve RA patients showed a significant expansion of IL-17A+, IFN?+ and IL-17A+IFN?+ CD4+T-lymphocyte subsets and increased intracellular STAT-1 and STAT-3 phosphorylation. Under basal conditions, nonresponder patients showed increased numbers of circulating IL-17A producing TN and TMC CD4+T-lymphocytes and IFN? producing TN, TCM, TEM CD4+T-lymphocytes with respect to responders. After 6 months, the numbers of CD4+IL-17A+TN remained significantly increased in nonresponders. In conclusion, CD4+T-lymphocytes in new-onset DMARD-naïve RA patients show IL-17A and IFN? abnormalities in TN, indicating their relevant role in early disease pathogenesis. Different patterns of CD4+ modulation are identified in MTX responders and nonresponders.Instituto de Salud Carlos IIIComunidad de Madrid20202020-09-18journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/63939https://dx.doi.org/10.3390/ijms2118684732961930reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3804 Medicina Individualizada Traslacional en Inflamación y CáncerNot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/639392026-06-18T11:13:07Z
dc.title.none.fl_str_mv Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
title Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
spellingShingle Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
Monserrat Sanz, Jorge|||0000-0003-1775-4645
Naïve rheumatoid arthritis
CD4+ T-lymphocytes
Methotrexate response
IFNgamma, IL-17A
STAT expression
Medicina
Medicine
title_short Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
title_full Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
title_fullStr Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
title_full_unstemmed Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
title_sort Methrotexate treatment inmunomodulates abnormal cytokine expression by T CD4 lymphocytes present in DMARD-Naïve Rheumatoid Arthritis Patients
dc.creator.none.fl_str_mv Monserrat Sanz, Jorge|||0000-0003-1775-4645
Álvarez De Mon Soto, Melchor|||0000-0003-1309-7510
Bohorquez Heras, Cristina|||0000-0002-1601-2079
Gómez Lahoz, Ana María|||0000-0003-3981-5138
Movasat Hajkhan, Atusa
Pérez Gómez, Ana María
Ruiz Sanjuán, Lucía
Díaz Martín, David|||0000-0002-5672-6658
Sánchez Atrio, Ana Isabel
Albarrán Hernández, Fernando
Sanz Paz, Ignacio
author Monserrat Sanz, Jorge|||0000-0003-1775-4645
author_facet Monserrat Sanz, Jorge|||0000-0003-1775-4645
Álvarez De Mon Soto, Melchor|||0000-0003-1309-7510
Bohorquez Heras, Cristina|||0000-0002-1601-2079
Gómez Lahoz, Ana María|||0000-0003-3981-5138
Movasat Hajkhan, Atusa
Pérez Gómez, Ana María
Ruiz Sanjuán, Lucía
Díaz Martín, David|||0000-0002-5672-6658
Sánchez Atrio, Ana Isabel
Albarrán Hernández, Fernando
Sanz Paz, Ignacio
author_role author
author2 Álvarez De Mon Soto, Melchor|||0000-0003-1309-7510
Bohorquez Heras, Cristina|||0000-0002-1601-2079
Gómez Lahoz, Ana María|||0000-0003-3981-5138
Movasat Hajkhan, Atusa
Pérez Gómez, Ana María
Ruiz Sanjuán, Lucía
Díaz Martín, David|||0000-0002-5672-6658
Sánchez Atrio, Ana Isabel
Albarrán Hernández, Fernando
Sanz Paz, Ignacio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Naïve rheumatoid arthritis
CD4+ T-lymphocytes
Methotrexate response
IFNgamma, IL-17A
STAT expression
Medicina
Medicine
topic Naïve rheumatoid arthritis
CD4+ T-lymphocytes
Methotrexate response
IFNgamma, IL-17A
STAT expression
Medicina
Medicine
description 17 p.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-09-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/63939
https://dx.doi.org/10.3390/ijms21186847
32961930
url http://hdl.handle.net/10017/63939
https://dx.doi.org/10.3390/ijms21186847
identifier_str_mv 32961930
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3804 Medicina Individualizada Traslacional en Inflamación y Cáncer
Not available Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
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