Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry

Bennett, Teresa A.; Montesinos, Pau|||0000-0002-3275-5593; Moscardo, Federico; Martinez-Cuadron, David; Martinez, Joaquin; Sierra, Jorge|||0000-0002-7966-0356; García, Raimundo; Perez de Oteyza, Jaime|||0000-0002-8338-2037; Fernandez, Pascual; Serrano, Josefina; Fernandez, Angeles; Herrera, Pilar|||0000-0002-4118-7110; Gonzalez, Ataulfo; Bethancourt, Concepción; Rodriguez-Macias, Gabriela; Alonso, Arancha; Vera, Juan A.; Navas Elorza, Begona; Lavilla, Esperanza|||0000-0003-1490-0678; López, Juan A.; Jiménez, Santiago; Simiele, Adriana; Vidriales, Belen; González, Bernardo J.; Burgaleta, Carmen; Hernández Rivas, José A.; Córdoba Mascuñano, Raúl; Bautista, Guiomar; Perez Simon, José A.; De la Fuente, Adolfo; Rayón, Consolación; Troconiz, Iñaki|||0000-0003-3700-8658; Janda, Álvaro; Bosanquet, Andrew G.; Hernández-Campo, Pilar; Primo, Daniel; López, Rocío; Liébana, Belen; Rojas, José L.; Gorrochategui, Julián|||0000-0001-9262-8716; Sanz, Miguel A.|||0000-0003-1489-1177; Ballesteros, Joan

Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry

Background We have evaluated the ex vivo pharmacology of single drugs and drug combinations in malignant cells of bone marrow samples from 125 patients with acute myeloid leukemia using a novel automated flow cytometry-based platform (ExviTech). We have improved previous ex vivo drug testing with 4...

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Autores:	Bennett, Teresa A., Montesinos, Pau\|\|\|0000-0002-3275-5593, Moscardo, Federico, Martinez-Cuadron, David, Martinez, Joaquin, Sierra, Jorge\|\|\|0000-0002-7966-0356, García, Raimundo, Perez de Oteyza, Jaime\|\|\|0000-0002-8338-2037, Fernandez, Pascual, Serrano, Josefina, Fernandez, Angeles, Herrera, Pilar\|\|\|0000-0002-4118-7110, Gonzalez, Ataulfo, Bethancourt, Concepción, Rodriguez-Macias, Gabriela, Alonso, Arancha, Vera, Juan A., Navas Elorza, Begona, Lavilla, Esperanza\|\|\|0000-0003-1490-0678, López, Juan A., Jiménez, Santiago, Simiele, Adriana, Vidriales, Belen, González, Bernardo J., Burgaleta, Carmen, Hernández Rivas, José A., Córdoba Mascuñano, Raúl, Bautista, Guiomar, Perez Simon, José A., De la Fuente, Adolfo, Rayón, Consolación, Troconiz, Iñaki\|\|\|0000-0003-3700-8658, Janda, Álvaro, Bosanquet, Andrew G., Hernández-Campo, Pilar, Primo, Daniel, López, Rocío, Liébana, Belen, Rojas, José L., Gorrochategui, Julián\|\|\|0000-0001-9262-8716, Sanz, Miguel A.\|\|\|0000-0003-1489-1177, Ballesteros, Joan
Tipo de recurso:	artículo
Fecha de publicación:	2014
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:301838
Acceso en línea:	https://ddd.uab.cat/record/301838 https://dx.doi.org/urn:doi:10.1016/j.clml.2013.11.006
Access Level:	acceso abierto
Palabra clave:	Chemosensitivity Ex vivo Flow cytometry Individualized tumor response to testing Personalized medicine

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spelling	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometryA bridge to individualized medicineBennett, Teresa A.Montesinos, Pau\|\|\|0000-0002-3275-5593Moscardo, FedericoMartinez-Cuadron, DavidMartinez, JoaquinSierra, Jorge\|\|\|0000-0002-7966-0356García, RaimundoPerez de Oteyza, Jaime\|\|\|0000-0002-8338-2037Fernandez, PascualSerrano, JosefinaFernandez, AngelesHerrera, Pilar\|\|\|0000-0002-4118-7110Gonzalez, AtaulfoBethancourt, ConcepciónRodriguez-Macias, GabrielaAlonso, AranchaVera, Juan A.Navas Elorza, BegonaLavilla, Esperanza\|\|\|0000-0003-1490-0678López, Juan A.Jiménez, SantiagoSimiele, AdrianaVidriales, BelenGonzález, Bernardo J.Burgaleta, CarmenHernández Rivas, José A.Córdoba Mascuñano, RaúlBautista, GuiomarPerez Simon, José A.De la Fuente, AdolfoRayón, ConsolaciónTroconiz, Iñaki\|\|\|0000-0003-3700-8658Janda, ÁlvaroBosanquet, Andrew G.Hernández-Campo, PilarPrimo, DanielLópez, RocíoLiébana, BelenRojas, José L.Gorrochategui, Julián\|\|\|0000-0001-9262-8716Sanz, Miguel A.\|\|\|0000-0003-1489-1177Ballesteros, JoanChemosensitivityEx vivoFlow cytometryIndividualized tumor response to testingPersonalized medicineBackground We have evaluated the ex vivo pharmacology of single drugs and drug combinations in malignant cells of bone marrow samples from 125 patients with acute myeloid leukemia using a novel automated flow cytometry-based platform (ExviTech). We have improved previous ex vivo drug testing with 4 innovations: identifying individual leukemic cells, using intact whole blood during the incubation, using an automated platform that escalates reliably data, and performing analyses pharmacodynamic population models. Patients and Methods Samples were sent from 24 hospitals to a central laboratory and incubated for 48 hours in whole blood, after which drug activity was measured in terms of depletion of leukemic cells. Results The sensitivity of single drugs is assessed for standard efficacy (E) and potency (EC) variables, ranked as percentiles within the population. The sensitivity of drug-combination treatments is assessed for the synergism achieved in each patient sample. We found a large variability among patient samples in the dose-response curves to a single drug or combination treatment. Conclusion We hypothesize that the use of the individual patient ex vivo pharmacological profiles may help to guide a personalized treatment selection. © 2014 The Authors. Published by Elsevier Inc. All rights reserved.Universitat Autònoma de Barcelona. Departament de Medicina 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/301838https://dx.doi.org/urn:doi:10.1016/j.clml.2013.11.006reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra, i la creació d'obres derivades, sempre que no sigui amb finalitats comercials i que es distribueixin sota la mateixa llicència que regula l'obra original. Cal que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3018382026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry A bridge to individualized medicine
title	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
spellingShingle	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry Bennett, Teresa A. Chemosensitivity Ex vivo Flow cytometry Individualized tumor response to testing Personalized medicine
title_short	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
title_full	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
title_fullStr	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
title_full_unstemmed	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
title_sort	Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry
dc.creator.none.fl_str_mv	Bennett, Teresa A. Montesinos, Pau\|\|\|0000-0002-3275-5593 Moscardo, Federico Martinez-Cuadron, David Martinez, Joaquin Sierra, Jorge\|\|\|0000-0002-7966-0356 García, Raimundo Perez de Oteyza, Jaime\|\|\|0000-0002-8338-2037 Fernandez, Pascual Serrano, Josefina Fernandez, Angeles Herrera, Pilar\|\|\|0000-0002-4118-7110 Gonzalez, Ataulfo Bethancourt, Concepción Rodriguez-Macias, Gabriela Alonso, Arancha Vera, Juan A. Navas Elorza, Begona Lavilla, Esperanza\|\|\|0000-0003-1490-0678 López, Juan A. Jiménez, Santiago Simiele, Adriana Vidriales, Belen González, Bernardo J. Burgaleta, Carmen Hernández Rivas, José A. Córdoba Mascuñano, Raúl Bautista, Guiomar Perez Simon, José A. De la Fuente, Adolfo Rayón, Consolación Troconiz, Iñaki\|\|\|0000-0003-3700-8658 Janda, Álvaro Bosanquet, Andrew G. Hernández-Campo, Pilar Primo, Daniel López, Rocío Liébana, Belen Rojas, José L. Gorrochategui, Julián\|\|\|0000-0001-9262-8716 Sanz, Miguel A.\|\|\|0000-0003-1489-1177 Ballesteros, Joan
author	Bennett, Teresa A.
author_facet	Bennett, Teresa A. Montesinos, Pau\|\|\|0000-0002-3275-5593 Moscardo, Federico Martinez-Cuadron, David Martinez, Joaquin Sierra, Jorge\|\|\|0000-0002-7966-0356 García, Raimundo Perez de Oteyza, Jaime\|\|\|0000-0002-8338-2037 Fernandez, Pascual Serrano, Josefina Fernandez, Angeles Herrera, Pilar\|\|\|0000-0002-4118-7110 Gonzalez, Ataulfo Bethancourt, Concepción Rodriguez-Macias, Gabriela Alonso, Arancha Vera, Juan A. Navas Elorza, Begona Lavilla, Esperanza\|\|\|0000-0003-1490-0678 López, Juan A. Jiménez, Santiago Simiele, Adriana Vidriales, Belen González, Bernardo J. Burgaleta, Carmen Hernández Rivas, José A. Córdoba Mascuñano, Raúl Bautista, Guiomar Perez Simon, José A. De la Fuente, Adolfo Rayón, Consolación Troconiz, Iñaki\|\|\|0000-0003-3700-8658 Janda, Álvaro Bosanquet, Andrew G. Hernández-Campo, Pilar Primo, Daniel López, Rocío Liébana, Belen Rojas, José L. Gorrochategui, Julián\|\|\|0000-0001-9262-8716 Sanz, Miguel A.\|\|\|0000-0003-1489-1177 Ballesteros, Joan
author_role	author
author2	Montesinos, Pau\|\|\|0000-0002-3275-5593 Moscardo, Federico Martinez-Cuadron, David Martinez, Joaquin Sierra, Jorge\|\|\|0000-0002-7966-0356 García, Raimundo Perez de Oteyza, Jaime\|\|\|0000-0002-8338-2037 Fernandez, Pascual Serrano, Josefina Fernandez, Angeles Herrera, Pilar\|\|\|0000-0002-4118-7110 Gonzalez, Ataulfo Bethancourt, Concepción Rodriguez-Macias, Gabriela Alonso, Arancha Vera, Juan A. Navas Elorza, Begona Lavilla, Esperanza\|\|\|0000-0003-1490-0678 López, Juan A. Jiménez, Santiago Simiele, Adriana Vidriales, Belen González, Bernardo J. Burgaleta, Carmen Hernández Rivas, José A. Córdoba Mascuñano, Raúl Bautista, Guiomar Perez Simon, José A. De la Fuente, Adolfo Rayón, Consolación Troconiz, Iñaki\|\|\|0000-0003-3700-8658 Janda, Álvaro Bosanquet, Andrew G. Hernández-Campo, Pilar Primo, Daniel López, Rocío Liébana, Belen Rojas, José L. Gorrochategui, Julián\|\|\|0000-0001-9262-8716 Sanz, Miguel A.\|\|\|0000-0003-1489-1177 Ballesteros, Joan
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Universitat Autònoma de Barcelona. Departament de Medicina
dc.subject.none.fl_str_mv	Chemosensitivity Ex vivo Flow cytometry Individualized tumor response to testing Personalized medicine
topic	Chemosensitivity Ex vivo Flow cytometry Individualized tumor response to testing Personalized medicine
description	Background We have evaluated the ex vivo pharmacology of single drugs and drug combinations in malignant cells of bone marrow samples from 125 patients with acute myeloid leukemia using a novel automated flow cytometry-based platform (ExviTech). We have improved previous ex vivo drug testing with 4 innovations: identifying individual leukemic cells, using intact whole blood during the incubation, using an automated platform that escalates reliably data, and performing analyses pharmacodynamic population models. Patients and Methods Samples were sent from 24 hospitals to a central laboratory and incubated for 48 hours in whole blood, after which drug activity was measured in terms of depletion of leukemic cells. Results The sensitivity of single drugs is assessed for standard efficacy (E) and potency (EC) variables, ranked as percentiles within the population. The sensitivity of drug-combination treatments is assessed for the synergism achieved in each patient sample. We found a large variability among patient samples in the dose-response curves to a single drug or combination treatment. Conclusion We hypothesize that the use of the individual patient ex vivo pharmacological profiles may help to guide a personalized treatment selection. © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
publishDate	2014
dc.date.none.fl_str_mv	2 2014-01-01 2014 2014-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/301838 https://dx.doi.org/urn:doi:10.1016/j.clml.2013.11.006
url	https://ddd.uab.cat/record/301838 https://dx.doi.org/urn:doi:10.1016/j.clml.2013.11.006
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv	openAccess
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dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
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Pharmacological profiles of acute myeloid leukemia treatments in patient samples by automated flow cytometry

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