Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers

Cells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health and disease. In single adhesive cells, mechanotransduction involves the transmission of force from the extracellular matrix to the cell nucleus, where it affects nucleocytoplasmic transport...

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Authors: Granero Moya, Ignasi, Venturini, Valeria, Belthier, G., Groenen, Bart, Molina Jordán, Marc, González Martín, Miguel, Trepat Guixer, Xavier, Rheenen, J. van, Andreu, I., Roca-Cusachs Soulere, Pere
Format: article
Status:Published version
Publication Date:2024
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/215092
Online Access:https://hdl.handle.net/2445/215092
Access Level:Open access
Keyword:Transducció de senyal cel·lular
Proteïnes
Cellular signal transduction
Proteins
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spelling Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayersGranero Moya, IgnasiVenturini, ValeriaBelthier, G.Groenen, BartMolina Jordán, MarcGonzález Martín, MiguelTrepat Guixer, XavierRheenen, J. vanAndreu, I.Roca-Cusachs Soulere, PereTransducció de senyal cel·lularProteïnesCellular signal transductionProteinsCells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health and disease. In single adhesive cells, mechanotransduction involves the transmission of force from the extracellular matrix to the cell nucleus, where it affects nucleocytoplasmic transport (NCT) and the subsequent nuclear localization of transcriptional regulators such as YAP. However, if and how NCT is mechanosensitive in multicellular systems is unclear. Here, we characterize and use a fluorescent sensor of nucleocytoplasmic transport (Sencyt) and demonstrate that nucleocytoplasmic transport responds to mechanics but not cell density in cell monolayers. Using monolayers of both epithelial and mesenchymal phenotype, we show that NCT is altered in response both to osmotic shocks, and to the inhibition of cell contractility. Further, NCT correlates with the degree of nuclear deformation measured through nuclear solidity, a shape parameter related to nuclear envelope tension. In contrast, YAP but NCT is sensitive to cell density, showing that YAP response to cell-cell contacts is not via a mere mechanical effect of NCT. Our results demonstrate the generality of the mechanical regulation of NCT.2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/215092Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1242/jcs.262363Journal of Cell Science, 2024, vol. 137, num. 17https://doi.org/10.1242/jcs.262363cc by (c) Granero Moya, Ignasi et al, 2024http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2150922026-05-27T06:46:51Z
dc.title.none.fl_str_mv Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
title Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
spellingShingle Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
Granero Moya, Ignasi
Transducció de senyal cel·lular
Proteïnes
Cellular signal transduction
Proteins
title_short Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
title_full Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
title_fullStr Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
title_full_unstemmed Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
title_sort Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers
dc.creator.none.fl_str_mv Granero Moya, Ignasi
Venturini, Valeria
Belthier, G.
Groenen, Bart
Molina Jordán, Marc
González Martín, Miguel
Trepat Guixer, Xavier
Rheenen, J. van
Andreu, I.
Roca-Cusachs Soulere, Pere
author Granero Moya, Ignasi
author_facet Granero Moya, Ignasi
Venturini, Valeria
Belthier, G.
Groenen, Bart
Molina Jordán, Marc
González Martín, Miguel
Trepat Guixer, Xavier
Rheenen, J. van
Andreu, I.
Roca-Cusachs Soulere, Pere
author_role author
author2 Venturini, Valeria
Belthier, G.
Groenen, Bart
Molina Jordán, Marc
González Martín, Miguel
Trepat Guixer, Xavier
Rheenen, J. van
Andreu, I.
Roca-Cusachs Soulere, Pere
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Transducció de senyal cel·lular
Proteïnes
Cellular signal transduction
Proteins
topic Transducció de senyal cel·lular
Proteïnes
Cellular signal transduction
Proteins
description Cells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health and disease. In single adhesive cells, mechanotransduction involves the transmission of force from the extracellular matrix to the cell nucleus, where it affects nucleocytoplasmic transport (NCT) and the subsequent nuclear localization of transcriptional regulators such as YAP. However, if and how NCT is mechanosensitive in multicellular systems is unclear. Here, we characterize and use a fluorescent sensor of nucleocytoplasmic transport (Sencyt) and demonstrate that nucleocytoplasmic transport responds to mechanics but not cell density in cell monolayers. Using monolayers of both epithelial and mesenchymal phenotype, we show that NCT is altered in response both to osmotic shocks, and to the inhibition of cell contractility. Further, NCT correlates with the degree of nuclear deformation measured through nuclear solidity, a shape parameter related to nuclear envelope tension. In contrast, YAP but NCT is sensitive to cell density, showing that YAP response to cell-cell contacts is not via a mere mechanical effect of NCT. Our results demonstrate the generality of the mechanical regulation of NCT.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/215092
url https://hdl.handle.net/2445/215092
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1242/jcs.262363
Journal of Cell Science, 2024, vol. 137, num. 17
https://doi.org/10.1242/jcs.262363
dc.rights.none.fl_str_mv cc by (c) Granero Moya, Ignasi et al, 2024
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Granero Moya, Ignasi et al, 2024
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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