The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells

Rock bream iridovirus (RBIV) causes severe mass mortality in Korean rock bream (Oplegnathus fasciatus) populations. To date, immune defense mechanisms of rock bream against RBIV are unclear. While red blood cells (RBCs) are known to be involved in the immune response against viral infections, the pa...

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Authors: Jung, Myung-Hwa, CHICO GRAS, VERONICA, Ciordia, Sergio, Mena, Maria Carmen, Jung, Sung-Ju, Ortega-Villaizan, Maria del Mar
Format: article
Publication Date:2019
Country:España
Institution:Universidad Miguel Hernández de Elche
Repository:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/34452
Online Access:https://hdl.handle.net/11000/34452
Access Level:Open access
Keyword:rock bream
RBIV
red blood cells
erythrocyte
proteome
MHC class I
apoptosis
ISG15
CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadas
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oai_identifier_str oai:dspace.umh.es:11000/34452
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spelling The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood CellsJung, Myung-HwaCHICO GRAS, VERONICACiordia, SergioMena, Maria CarmenJung, Sung-JuOrtega-Villaizan, Maria del Marrock breamRBIVred blood cellserythrocyteproteomeMHC class IapoptosisISG15CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadasRock bream iridovirus (RBIV) causes severe mass mortality in Korean rock bream (Oplegnathus fasciatus) populations. To date, immune defense mechanisms of rock bream against RBIV are unclear. While red blood cells (RBCs) are known to be involved in the immune response against viral infections, the participation of rock bream RBCs in the immune response against RBIV has not been studied yet. In this study, we examined induction of the immune response in rock bream RBCs after RBIV infection. Each fish was injected with RBIV, and virus copy number in RBCs gradually increased from 4 days post-infection (dpi), peaking at 10 dpi. A total of 318 proteins were significantly regulated in RBCs from RBIV-infected individuals, 183 proteins were upregulated and 135 proteins were downregulated. Differentially upregulated proteins included those involved in cellular amino acid metabolic processes, cellular detoxification, snRNP assembly, and the spliceosome. Remarkably, the MHC class I-related protein pathway was upregulated during RBIV infection. Simultaneously, the regulation of apoptosis-related proteins, including caspase-6 (CASP6), caspase-9 (CASP9), Fas cell surface death receptor (FAS), desmoplakin (DSP), and p21 (RAC1)activated kinase 2 (PAK2) changed with RBIV infection. Interestingly, the expression of genes within the ISG15 antiviral mechanism-related pathway, including filamin B (FLNB), interferon regulatory factor 3 (IRF3), nucleoporin 35 (NUP35), tripartite motif-containing 25 (TRIM25), and karyopherin subunit alpha 3 (KPNA3) were downregulated in RBCs from RBIV-infected individuals. Overall, these findings contribute to the understanding of RBIV pathogenesis and host interaction.Frontiers MediaInstitutos de la UMH::Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche202520252019info:eu-repo/semantics/articleapplication/pdf14application/pdfhttps://hdl.handle.net/11000/34452reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.3389/fimmu.2019.00160info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/344522026-05-27T13:36:21Z
dc.title.none.fl_str_mv The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
title The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
spellingShingle The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
Jung, Myung-Hwa
rock bream
RBIV
red blood cells
erythrocyte
proteome
MHC class I
apoptosis
ISG15
CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadas
title_short The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
title_full The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
title_fullStr The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
title_full_unstemmed The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
title_sort The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells
dc.creator.none.fl_str_mv Jung, Myung-Hwa
CHICO GRAS, VERONICA
Ciordia, Sergio
Mena, Maria Carmen
Jung, Sung-Ju
Ortega-Villaizan, Maria del Mar
author Jung, Myung-Hwa
author_facet Jung, Myung-Hwa
CHICO GRAS, VERONICA
Ciordia, Sergio
Mena, Maria Carmen
Jung, Sung-Ju
Ortega-Villaizan, Maria del Mar
author_role author
author2 CHICO GRAS, VERONICA
Ciordia, Sergio
Mena, Maria Carmen
Jung, Sung-Ju
Ortega-Villaizan, Maria del Mar
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Institutos de la UMH::Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche
dc.subject.none.fl_str_mv rock bream
RBIV
red blood cells
erythrocyte
proteome
MHC class I
apoptosis
ISG15
CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadas
topic rock bream
RBIV
red blood cells
erythrocyte
proteome
MHC class I
apoptosis
ISG15
CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadas
description Rock bream iridovirus (RBIV) causes severe mass mortality in Korean rock bream (Oplegnathus fasciatus) populations. To date, immune defense mechanisms of rock bream against RBIV are unclear. While red blood cells (RBCs) are known to be involved in the immune response against viral infections, the participation of rock bream RBCs in the immune response against RBIV has not been studied yet. In this study, we examined induction of the immune response in rock bream RBCs after RBIV infection. Each fish was injected with RBIV, and virus copy number in RBCs gradually increased from 4 days post-infection (dpi), peaking at 10 dpi. A total of 318 proteins were significantly regulated in RBCs from RBIV-infected individuals, 183 proteins were upregulated and 135 proteins were downregulated. Differentially upregulated proteins included those involved in cellular amino acid metabolic processes, cellular detoxification, snRNP assembly, and the spliceosome. Remarkably, the MHC class I-related protein pathway was upregulated during RBIV infection. Simultaneously, the regulation of apoptosis-related proteins, including caspase-6 (CASP6), caspase-9 (CASP9), Fas cell surface death receptor (FAS), desmoplakin (DSP), and p21 (RAC1)activated kinase 2 (PAK2) changed with RBIV infection. Interestingly, the expression of genes within the ISG15 antiviral mechanism-related pathway, including filamin B (FLNB), interferon regulatory factor 3 (IRF3), nucleoporin 35 (NUP35), tripartite motif-containing 25 (TRIM25), and karyopherin subunit alpha 3 (KPNA3) were downregulated in RBCs from RBIV-infected individuals. Overall, these findings contribute to the understanding of RBIV pathogenesis and host interaction.
publishDate 2019
dc.date.none.fl_str_mv 2019
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/34452
url https://hdl.handle.net/11000/34452
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3389/fimmu.2019.00160
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
14
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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