Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937

40 p.-7 fig.-1 tab.

Bibliographic Details
Authors: Blanco, Francisco J., Ojeda-Fernández, María Luisa, Aristorena, Mikel, Gallardo-Vara, Eunate, Benguria, Alberto, Dopazo, Ana, Langa, Carmen, Botella, Luisa María, Bernabéu, Carmelo
Format: article
Status:Versión aceptada para publicación
Publication Date:2015
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/212893
Online Access:http://hdl.handle.net/10261/212893
Access Level:Open access
Keyword:Monocytes
Endoglin
Gene expression
Integrins
Activin A
Cell adhesion
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spelling Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937Running head: Endoglin isoforms in myeloid cellsBlanco, Francisco J.Ojeda-Fernández, María LuisaAristorena, MikelGallardo-Vara, EunateBenguria, AlbertoDopazo, AnaLanga, CarmenBotella, Luisa MaríaBernabéu, CarmeloMonocytesEndoglinGene expressionIntegrinsActivin ACell adhesion40 p.-7 fig.-1 tab.Endoglin is an auxiliary cell surface receptor for TGF-beta family members. Two different alternatively spliced isoforms, long (L)-endoglin and short (S)-endoglin, have been reported. S-endoglin and L-endoglin proteins vary from each other in their cytoplasmic tails that contain 14 and 47 amino acids, respectively. A critical role for endoglin in vascular development has primarily been studied in endothelial cells. In addition, endoglin expression is upregulated during monocyte-to-macrophage differentiation; however, little is known about its role in this myeloid context. To investigate the function of endoglin in monocytes, stable transfectants expressing the two endoglin isoforms in the promonocytic human cell line U937 were generated. The differential gene expression fingerprinting of these endoglin transfectants using DNA microarrays and further bioinformatics analysis showed a clear alteration in essential biological functions, mainly those related to "Cellular Movement", including cell adhesion and transmigration. Interestingly, these cellular functions are highly dependent on adhesion molecules, including integrins alpha 1 (CD49a, ITGA1 gene), alpha L (CD11a, ITGAL gene), alpha M(CD11b, ITGAM gene) and beta 2 (CD18, ITGB2 gene) and the chemokine receptor CCR2 (CD192, CCR2 gene), which are downregulated in endoglin transfectants. Moreover, activin A (INHBA gene), a TGF-beta superfamily member involved in macrophage polarization, was distinctly affected in each endoglin transfectant, and may contribute to the regulated expression of integrins. These data were confirmed by quantitative PCR, flow cytometry and functional tests. Taken together, these results provide new insight into endoglin function in monocytes.This study has been supported by grants from Ministerio de Economia y Competitividad of Spain (SAF2010-19222 to CB), Genoma España (MEICA to CB), and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER, CB). FJB is a scientific researcher from the CIBERER, an initiative of the Instituto de Salud Carlos III (ISCIII) of Spain supported by FEDER funds.Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Fundación Genoma EspañaCentro de Investigación Biomédica en Red Enfermedades Raras (España)Instituto de Salud Carlos IIIEuropean CommissionAristorena, Mikel 0000-0003-0147-2635Gallardo-Vara, Eunate [0000-0003-4733-0878]Benguria, Alberto [0000-0002-5536-566X]Dopazo, Ana [0000-0002-4910-1684]Botella, Luisa María [0000-0002-6310-2245]Bernabéu, Carmelo [0000-0002-1563-6162]Bernabéu, Carmelo [0000-0002-1563-6162]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/212893reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1002/jcp.24827Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2128932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
Running head: Endoglin isoforms in myeloid cells
title Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
spellingShingle Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
Blanco, Francisco J.
Monocytes
Endoglin
Gene expression
Integrins
Activin A
Cell adhesion
title_short Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
title_full Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
title_fullStr Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
title_full_unstemmed Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
title_sort Genome-wide transcriptional and functional analysis of endoglin isoforms in the human promonocytic cell line U937
dc.creator.none.fl_str_mv Blanco, Francisco J.
Ojeda-Fernández, María Luisa
Aristorena, Mikel
Gallardo-Vara, Eunate
Benguria, Alberto
Dopazo, Ana
Langa, Carmen
Botella, Luisa María
Bernabéu, Carmelo
author Blanco, Francisco J.
author_facet Blanco, Francisco J.
Ojeda-Fernández, María Luisa
Aristorena, Mikel
Gallardo-Vara, Eunate
Benguria, Alberto
Dopazo, Ana
Langa, Carmen
Botella, Luisa María
Bernabéu, Carmelo
author_role author
author2 Ojeda-Fernández, María Luisa
Aristorena, Mikel
Gallardo-Vara, Eunate
Benguria, Alberto
Dopazo, Ana
Langa, Carmen
Botella, Luisa María
Bernabéu, Carmelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Genoma España
Centro de Investigación Biomédica en Red Enfermedades Raras (España)
Instituto de Salud Carlos III
European Commission
Aristorena, Mikel 0000-0003-0147-2635
Gallardo-Vara, Eunate [0000-0003-4733-0878]
Benguria, Alberto [0000-0002-5536-566X]
Dopazo, Ana [0000-0002-4910-1684]
Botella, Luisa María [0000-0002-6310-2245]
Bernabéu, Carmelo [0000-0002-1563-6162]
Bernabéu, Carmelo [0000-0002-1563-6162]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Monocytes
Endoglin
Gene expression
Integrins
Activin A
Cell adhesion
topic Monocytes
Endoglin
Gene expression
Integrins
Activin A
Cell adhesion
description 40 p.-7 fig.-1 tab.
publishDate 2015
dc.date.none.fl_str_mv 2015
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/212893
url http://hdl.handle.net/10261/212893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1002/jcp.24827
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412586530799616
score 15.812429

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