Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots

Understanding stem cell regulatory circuits is the next challenge in plant biology, as these cells are essential for tissue growth and organ regeneration in response to stress. In the Arabidopsis primary root apex, stem cell-specific transcription factors BRAVO and WOX5 co-localize in the quiescent...

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Autores: Betegón-Putze, Isabel, Mercadal, Josep, Bosch, Nadja, Planas-Riverola, Ainoa, Marquès-Bueno, Maria Mar, Vilarrasa-Blasi, Josep, Frigola, David, Burkart, Rebecca C., Martínez, Cristina, Conesa, Ana, Sozzani, Rosangela, Stahl, Yvonne, Prat, Salomé, Ibañes, Marta, Caño-Delgado, Ana I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/253942
Acceso en línea:http://hdl.handle.net/10261/253942
Access Level:acceso abierto
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spelling Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis rootsBetegón-Putze, IsabelMercadal, JosepBosch, NadjaPlanas-Riverola, AinoaMarquès-Bueno, Maria MarVilarrasa-Blasi, JosepFrigola, DavidBurkart, Rebecca C.Martínez, CristinaConesa, AnaSozzani, RosangelaStahl, YvonnePrat, SaloméIbañes, MartaCaño-Delgado, Ana I.Understanding stem cell regulatory circuits is the next challenge in plant biology, as these cells are essential for tissue growth and organ regeneration in response to stress. In the Arabidopsis primary root apex, stem cell-specific transcription factors BRAVO and WOX5 co-localize in the quiescent centre (QC) cells, where they commonly repress cell division so that these cells can act as a reservoir to replenish surrounding stem cells, yet their molecular connection remains unknown. Genetic and biochemical analysis indicates that BRAVO and WOX5 form a transcription factor complex that modulates gene expression in the QC cells to preserve overall root growth and architecture. Furthermore, by using mathematical modelling we establish that BRAVO uses the WOX5/BRAVO complex to promote WOX5 activity in the stem cells. Our results unveil the importance of transcriptional regulatory circuits in plant stem cell development.A.I.C-D. is a recipient of a BIO2016-78955 grant from the Spanish Ministry of Economy and Competitiveness and a European Research Council, ERC Consolidator Grant (ERC-2015-CoG – 683163). I.B-P. is funded by the FPU15/02822 grant from the Spanish Ministry of Education, Culture and Sport; N.B. by the FI-DGR 2016FI_B 00472 grant from the AGAUR, Generalitat de Catalunya; and A.P-R. by the SEV-2015-0533 from the Severo Ochoa Programme for Centers of Excellence in R&D. M.I. and J.M. acknowledge support from the Spanish Ministry of Economy and Competitiveness and FEDER (EU) through grant FIS2015-66503-C3-3-P, from Ministerio de Ciencia, Innovación y Universidades / Agencia Estatal de Investigación / Fondo Europeo de Desarrollo Regional, Unión Europea through grant PGC2018-101896-B-I00 and from the Generalitat de Catalunya through Grup de Recerca Consolidat 2014 SGR 878 and 2017 SGR 1061. J.M. is funded by the Spanish Ministry of Education through BES-2016-078218. Y.S. and R.C.D. were funded by the Deutsche Forschungsgesellschaft (DFG) (grant STA12/12 1-1). A.I.C.-D.–A.C. collaboration was funded by the European Regional Development Funds and Marie Curie IRSES Project DEANN (PIRSES-GA-2013-612583). CRAG is funded by “Severo Ochoa Programme” from Centers of Excellence in R&D 2016-2019 (SEV-2015-485 0533).Peer reviewedEMBO PressMinisterio de Economía y Competitividad (España)European Research CouncilEuropean CommissionMinisterio de Educación, Cultura y Deporte (España)Generalitat de CatalunyaGerman Research FoundationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/253942reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-78955-Pinfo:eu-repo/grantAgreement/EC/H2020/683163info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0533info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2015-66503-C3-3-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-101896-B-I00https://doi.org/10.15252/msb.20209864Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2539422026-05-22T06:33:51Z
dc.title.none.fl_str_mv Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
title Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
spellingShingle Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
Betegón-Putze, Isabel
title_short Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
title_full Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
title_fullStr Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
title_full_unstemmed Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
title_sort Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
dc.creator.none.fl_str_mv Betegón-Putze, Isabel
Mercadal, Josep
Bosch, Nadja
Planas-Riverola, Ainoa
Marquès-Bueno, Maria Mar
Vilarrasa-Blasi, Josep
Frigola, David
Burkart, Rebecca C.
Martínez, Cristina
Conesa, Ana
Sozzani, Rosangela
Stahl, Yvonne
Prat, Salomé
Ibañes, Marta
Caño-Delgado, Ana I.
author Betegón-Putze, Isabel
author_facet Betegón-Putze, Isabel
Mercadal, Josep
Bosch, Nadja
Planas-Riverola, Ainoa
Marquès-Bueno, Maria Mar
Vilarrasa-Blasi, Josep
Frigola, David
Burkart, Rebecca C.
Martínez, Cristina
Conesa, Ana
Sozzani, Rosangela
Stahl, Yvonne
Prat, Salomé
Ibañes, Marta
Caño-Delgado, Ana I.
author_role author
author2 Mercadal, Josep
Bosch, Nadja
Planas-Riverola, Ainoa
Marquès-Bueno, Maria Mar
Vilarrasa-Blasi, Josep
Frigola, David
Burkart, Rebecca C.
Martínez, Cristina
Conesa, Ana
Sozzani, Rosangela
Stahl, Yvonne
Prat, Salomé
Ibañes, Marta
Caño-Delgado, Ana I.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Research Council
European Commission
Ministerio de Educación, Cultura y Deporte (España)
Generalitat de Catalunya
German Research Foundation
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Understanding stem cell regulatory circuits is the next challenge in plant biology, as these cells are essential for tissue growth and organ regeneration in response to stress. In the Arabidopsis primary root apex, stem cell-specific transcription factors BRAVO and WOX5 co-localize in the quiescent centre (QC) cells, where they commonly repress cell division so that these cells can act as a reservoir to replenish surrounding stem cells, yet their molecular connection remains unknown. Genetic and biochemical analysis indicates that BRAVO and WOX5 form a transcription factor complex that modulates gene expression in the QC cells to preserve overall root growth and architecture. Furthermore, by using mathematical modelling we establish that BRAVO uses the WOX5/BRAVO complex to promote WOX5 activity in the stem cells. Our results unveil the importance of transcriptional regulatory circuits in plant stem cell development.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/253942
url http://hdl.handle.net/10261/253942
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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#PLACEHOLDER_PARENT_METADATA_VALUE#
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#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-78955-P
info:eu-repo/grantAgreement/EC/H2020/683163
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0533
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2015-66503-C3-3-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-101896-B-I00
https://doi.org/10.15252/msb.20209864
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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