Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids

In the current study, a smart release system responsive to temperature was developed to improve the efficiency of tetracycline (TC) in antibacterial therapy. The nanovesicles designed consist of a non-ionic surfactant, SPAN60, cholesterol and a phase change material (PCM) as a thermoresponsive gatin...

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Autores: Mazzotta, Elisabetta, Romeo, Martina, Hafidi, Zakaria, Pérez, Lourdes, Perrotta, Ida Daniela, Muzzalupo, Rita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/365124
Acceso en línea:http://hdl.handle.net/10261/365124
Access Level:acceso abierto
Palabra clave:Phase change materials
Natural fatty acids
Niosomes
Thermoresponsive release
Antibacterial activity
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spelling Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty AcidsMazzotta, ElisabettaRomeo, MartinaHafidi, ZakariaPérez, LourdesPerrotta, Ida DanielaMuzzalupo, RitaPhase change materialsNatural fatty acidsNiosomesThermoresponsive releaseAntibacterial activityIn the current study, a smart release system responsive to temperature was developed to improve the efficiency of tetracycline (TC) in antibacterial therapy. The nanovesicles designed consist of a non-ionic surfactant, SPAN60, cholesterol and a phase change material (PCM) as a thermoresponsive gating material. Niosomes were prepared using an increasing amount of PCM and characterized in terms of size, zeta potential, colloidal stability and thermoresponsive properties. The vesicles that developed were homogenous in size, had good biocompatibility and stability for up to 3 months and demonstrated thermoresponsive behavior. A low drug leakage was observed at 37 ◦C, while a rapid release occurred at 42 ◦C, due to the faster diffusion rate of the drug trough the melted PCM. This controllable drug release capacity allows us to avoid premature drug release, minimizing unwanted and toxic effects and ensuring a long retention time in the nanodevice so that it reaches the infected sites. In addition, TC-loaded niosomes were screened to investigate their antibacterial activity against various Gram-positive and Gram-negative bacteria by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. An interesting temperature-dependent antibacterial activity was observed against some bacterial strains: the niosomes activity against S. epidermis, for example, was improved by the temperature increase, as suggested by a reduction in MIC values from 112.81 to 14.10 μM observed at 37 and 42 ◦C, respectively. Taken together, the thermoresponsive platform developed allows us to use lower antibiotic amounts while ensuring therapeutic efficacy and, so, will advance the development of a novel antibacterial agent in clinical practiceThis work was supported by PON R&I 2014-2020-ARS01_00568-SI.F.I.PA.CRO.DE; MUR, the Italian Ministry for University, is acknowledged for their financial support (EX-60%), and Sistema Integrato di Laboratori per L’Ambiente (SILA) for providing lab tools; PRIN 2020 (Progetti di Rilevante Interesse Nazionale) Grant 2020ENLMHA from MUR, Italy (S.A.); Grant PID2022-136354-NBI00 funded by MCIN/AEI/10.13039/5011000110Not 33 and by “ERDF”, a European fundPeer reviewedMultidisciplinary Digital Publishing InstituteMinistero dell'Istruzione, dell'Università e della RicercaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/365124reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136354NB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/pharmaceutics16070909https://doi.org/10.3390/pharmaceutics16070909Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3651242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
title Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
spellingShingle Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
Mazzotta, Elisabetta
Phase change materials
Natural fatty acids
Niosomes
Thermoresponsive release
Antibacterial activity
title_short Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
title_full Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
title_fullStr Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
title_full_unstemmed Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
title_sort Design of Thermosensitive Niosomes by Eutectic Mixture of Natural Fatty Acids
dc.creator.none.fl_str_mv Mazzotta, Elisabetta
Romeo, Martina
Hafidi, Zakaria
Pérez, Lourdes
Perrotta, Ida Daniela
Muzzalupo, Rita
author Mazzotta, Elisabetta
author_facet Mazzotta, Elisabetta
Romeo, Martina
Hafidi, Zakaria
Pérez, Lourdes
Perrotta, Ida Daniela
Muzzalupo, Rita
author_role author
author2 Romeo, Martina
Hafidi, Zakaria
Pérez, Lourdes
Perrotta, Ida Daniela
Muzzalupo, Rita
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministero dell'Istruzione, dell'Università e della Ricerca
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Phase change materials
Natural fatty acids
Niosomes
Thermoresponsive release
Antibacterial activity
topic Phase change materials
Natural fatty acids
Niosomes
Thermoresponsive release
Antibacterial activity
description In the current study, a smart release system responsive to temperature was developed to improve the efficiency of tetracycline (TC) in antibacterial therapy. The nanovesicles designed consist of a non-ionic surfactant, SPAN60, cholesterol and a phase change material (PCM) as a thermoresponsive gating material. Niosomes were prepared using an increasing amount of PCM and characterized in terms of size, zeta potential, colloidal stability and thermoresponsive properties. The vesicles that developed were homogenous in size, had good biocompatibility and stability for up to 3 months and demonstrated thermoresponsive behavior. A low drug leakage was observed at 37 ◦C, while a rapid release occurred at 42 ◦C, due to the faster diffusion rate of the drug trough the melted PCM. This controllable drug release capacity allows us to avoid premature drug release, minimizing unwanted and toxic effects and ensuring a long retention time in the nanodevice so that it reaches the infected sites. In addition, TC-loaded niosomes were screened to investigate their antibacterial activity against various Gram-positive and Gram-negative bacteria by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. An interesting temperature-dependent antibacterial activity was observed against some bacterial strains: the niosomes activity against S. epidermis, for example, was improved by the temperature increase, as suggested by a reduction in MIC values from 112.81 to 14.10 μM observed at 37 and 42 ◦C, respectively. Taken together, the thermoresponsive platform developed allows us to use lower antibiotic amounts while ensuring therapeutic efficacy and, so, will advance the development of a novel antibacterial agent in clinical practice
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/365124
url http://hdl.handle.net/10261/365124
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136354NB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/pharmaceutics16070909
https://doi.org/10.3390/pharmaceutics16070909
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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