Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes

The aim of this study was to investigate the cytoprotective effects of CT-1 against non-ischemic death stimuli in adult cardiomyocytes. Primary cultures of cardiomyocytes isolated from adult rats were stimulated with either angiotensin II (Ang II) or H(2)O(2) in the presence or absence of CT-1. Cell...

Full description

Bibliographic Details
Authors: Lopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91, Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41, Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
Format: article
Publication Date:2005
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/19841
Online Access:https://hdl.handle.net/10171/19841
Access Level:Open access
Keyword:Adult cardiomyocytes
Angiotensin II
Cardiotrophin-1
Oxidative stress
Survival
id ES_88689b03e4e978da7471d15968aeea33
oai_identifier_str oai:dadun.unav.edu:10171/19841
network_acronym_str ES
network_name_str España
repository_id_str
spelling Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytesLopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ecAdult cardiomyocytesAngiotensin IICardiotrophin-1Oxidative stressSurvivalThe aim of this study was to investigate the cytoprotective effects of CT-1 against non-ischemic death stimuli in adult cardiomyocytes. Primary cultures of cardiomyocytes isolated from adult rats were stimulated with either angiotensin II (Ang II) or H(2)O(2) in the presence or absence of CT-1. Cell death was determined by trypan blue exclusion, cell viability by MTT assay and apoptosis by TUNEL-Annexin-V staining. Intracellular pathways were analyzed by the employment of chemical inhibitors and by the assessment of signalling intermediates phosphorylation by Western blot analysis. CT-1 reduced (p<0.01) total cell death and apoptosis induced by either Ang II or H(2)O(2), and increased (p<0.01) cell viability in cardiomyocytes exposed to these stimuli. These effects of CT-1 were abolished in the presence of antibodies specific for gp130 or LIFR and did not require RNA or protein synthesis. Both Wortmannin and PD98059 abolished protective effects of CT-1 against H(2)O(2), whereas only Wortmannin inhibited protection against Ang II. In both cases, Akt kinase activation and Bad phosphorylation were observed. These findings suggest that CT-1 protects adult cardiomyocytes against Ang II- and oxidative stress-induced cell death, via gp130/LIFR and by means of the PI3K/Akt and the p42/44 MAPK intracellular cascades.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20112011-11-1620052005-01-0120052005-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/19841reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/198412026-06-21T12:47:57Z
dc.title.none.fl_str_mv Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
title Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
spellingShingle Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
Lopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91
Adult cardiomyocytes
Angiotensin II
Cardiotrophin-1
Oxidative stress
Survival
title_short Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
title_full Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
title_fullStr Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
title_full_unstemmed Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
title_sort Characterization of the protective effects of cardiotrophin-1 against non-ischemic death stimuli in adult cardiomyocytes
dc.creator.none.fl_str_mv Lopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91
Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author Lopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91
author_facet Lopez, N. (Natalia)|||/items/0edf59fe-771c-4a1c-98bf-cd0d8dd4cb91
Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author_role author
author2 Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author2_role author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Adult cardiomyocytes
Angiotensin II

Cardiotrophin-1
Oxidative stress
Survival
topic Adult cardiomyocytes
Angiotensin II
Cardiotrophin-1
Oxidative stress
Survival
description The aim of this study was to investigate the cytoprotective effects of CT-1 against non-ischemic death stimuli in adult cardiomyocytes. Primary cultures of cardiomyocytes isolated from adult rats were stimulated with either angiotensin II (Ang II) or H(2)O(2) in the presence or absence of CT-1. Cell death was determined by trypan blue exclusion, cell viability by MTT assay and apoptosis by TUNEL-Annexin-V staining. Intracellular pathways were analyzed by the employment of chemical inhibitors and by the assessment of signalling intermediates phosphorylation by Western blot analysis. CT-1 reduced (p<0.01) total cell death and apoptosis induced by either Ang II or H(2)O(2), and increased (p<0.01) cell viability in cardiomyocytes exposed to these stimuli. These effects of CT-1 were abolished in the presence of antibodies specific for gp130 or LIFR and did not require RNA or protein synthesis. Both Wortmannin and PD98059 abolished protective effects of CT-1 against H(2)O(2), whereas only Wortmannin inhibited protection against Ang II. In both cases, Akt kinase activation and Bad phosphorylation were observed. These findings suggest that CT-1 protects adult cardiomyocytes against Ang II- and oxidative stress-induced cell death, via gp130/LIFR and by means of the PI3K/Akt and the p42/44 MAPK intracellular cascades.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01
2005
2005-01-01
2011
2011-11-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/19841
url https://hdl.handle.net/10171/19841
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412549519212544
score 15,300719

Similar Items