MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage

Brucellosis is a common zoonotic disease in Egypt. However, there are limited data available on the genetic diversity of brucellae circulating in Egypt and other Mediterranean areas. One hundred and nine Brucella (B.) strains were isolated from different animal species in thirteen Egyptian governora...

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Autores: Wareth, Gamal, El-Diasty, Mohamed, Melzer, Falk, Schmoock, Gernot, Moustafa, Shawky A., El-Beskawy, Mohamed, Khater, Dali F., Hamdy, Mahmoud E.R., Zaki, Hoda M., Ferreira, Ana Cristina, Ekateriniadou, Loukia V., Boukouvala, Evridiki, Abdel-Glil, Mostafa Y., Menshawy, Ahmed M.S., Pérez Sancho, Marta, Sakhria, Sonia, Pletz, Mathias W., Neubauer, Heinrich
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8374
Acceso en línea:https://hdl.handle.net/20.500.14352/8374
Access Level:acceso abierto
Palabra clave:B. melitensis
B. abortus
MLVA-16
genotyping
Egypt
Mediterranean Basin
Patología veterinaria
3109.07 Patología
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spelling MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean LineageWareth, GamalEl-Diasty, MohamedMelzer, FalkSchmoock, GernotMoustafa, Shawky A.El-Beskawy, MohamedKhater, Dali F.Hamdy, Mahmoud E.R.Zaki, Hoda M.Ferreira, Ana CristinaEkateriniadou, Loukia V.Boukouvala, EvridikiAbdel-Glil, Mostafa Y.Menshawy, Ahmed M.S.Pérez Sancho, MartaSakhria, SoniaPletz, Mathias W.Neubauer, HeinrichB. melitensisB. abortusMLVA-16genotypingEgyptMediterranean BasinPatología veterinaria3109.07 PatologíaBrucellosis is a common zoonotic disease in Egypt. However, there are limited data available on the genetic diversity of brucellae circulating in Egypt and other Mediterranean areas. One hundred and nine Brucella (B.) strains were isolated from different animal species in thirteen Egyptian governorates. Multi-locus variable number tandem repeats (VNTRs) analysis (MLVA-16) was employed to determine the geographical relatedness and the genetic diversity of a panel of selected Egyptian strains (n = 69), with strains originating from Italy (n = 49), Portugal (n = 52), Greece (n = 63), and Tunisia (n = 4). Egyptian B. melitensis strains clustered into two main clusters containing 21 genotypes. Egyptian B. abortus strains clustered into three main clusters containing nine genotypes. The genotypes were irregularly distributed over time and space in the study area. Egyptian strains of B. melitensis showed MLVA-16 patterns closer to that of Italian strains. Egyptian B. abortus strains isolated from cattle share the same genotype with strains from Portugal and similar to strains from Italy with low genetic diversity. Strains with similar MLVA patterns isolated from different governorates highlight the movement of the pathogen among governorates. Hence, it may also reflect the long endemicity of brucellosis in Egypt with earlier dispersal of types and great local genetic diversity. Open markets may contribute to cross-species transmission and dissemination of the new types nationwide. The presence of West Mediterranean lineages of B. melitensis and relatedness of B. abortus strains from the studied countries is a result of the socio-historical connections among the Mediterranean countries. Transnational eradication of brucellosis in the Mediterranean basin is highly demanded.MDPIUniversidad Complutense de Madrid20202020-06-2220202020-06-22journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8374reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/83742026-06-02T12:44:21Z
dc.title.none.fl_str_mv MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
title MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
spellingShingle MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
Wareth, Gamal
B. melitensis
B. abortus
MLVA-16
genotyping
Egypt
Mediterranean Basin
Patología veterinaria
3109.07 Patología
title_short MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
title_full MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
title_fullStr MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
title_full_unstemmed MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
title_sort MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates from Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage
dc.creator.none.fl_str_mv Wareth, Gamal
El-Diasty, Mohamed
Melzer, Falk
Schmoock, Gernot
Moustafa, Shawky A.
El-Beskawy, Mohamed
Khater, Dali F.
Hamdy, Mahmoud E.R.
Zaki, Hoda M.
Ferreira, Ana Cristina
Ekateriniadou, Loukia V.
Boukouvala, Evridiki
Abdel-Glil, Mostafa Y.
Menshawy, Ahmed M.S.
Pérez Sancho, Marta
Sakhria, Sonia
Pletz, Mathias W.
Neubauer, Heinrich
author Wareth, Gamal
author_facet Wareth, Gamal
El-Diasty, Mohamed
Melzer, Falk
Schmoock, Gernot
Moustafa, Shawky A.
El-Beskawy, Mohamed
Khater, Dali F.
Hamdy, Mahmoud E.R.
Zaki, Hoda M.
Ferreira, Ana Cristina
Ekateriniadou, Loukia V.
Boukouvala, Evridiki
Abdel-Glil, Mostafa Y.
Menshawy, Ahmed M.S.
Pérez Sancho, Marta
Sakhria, Sonia
Pletz, Mathias W.
Neubauer, Heinrich
author_role author
author2 El-Diasty, Mohamed
Melzer, Falk
Schmoock, Gernot
Moustafa, Shawky A.
El-Beskawy, Mohamed
Khater, Dali F.
Hamdy, Mahmoud E.R.
Zaki, Hoda M.
Ferreira, Ana Cristina
Ekateriniadou, Loukia V.
Boukouvala, Evridiki
Abdel-Glil, Mostafa Y.
Menshawy, Ahmed M.S.
Pérez Sancho, Marta
Sakhria, Sonia
Pletz, Mathias W.
Neubauer, Heinrich
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv B. melitensis
B. abortus
MLVA-16
genotyping
Egypt
Mediterranean Basin
Patología veterinaria
3109.07 Patología
topic B. melitensis
B. abortus
MLVA-16
genotyping
Egypt
Mediterranean Basin
Patología veterinaria
3109.07 Patología
description Brucellosis is a common zoonotic disease in Egypt. However, there are limited data available on the genetic diversity of brucellae circulating in Egypt and other Mediterranean areas. One hundred and nine Brucella (B.) strains were isolated from different animal species in thirteen Egyptian governorates. Multi-locus variable number tandem repeats (VNTRs) analysis (MLVA-16) was employed to determine the geographical relatedness and the genetic diversity of a panel of selected Egyptian strains (n = 69), with strains originating from Italy (n = 49), Portugal (n = 52), Greece (n = 63), and Tunisia (n = 4). Egyptian B. melitensis strains clustered into two main clusters containing 21 genotypes. Egyptian B. abortus strains clustered into three main clusters containing nine genotypes. The genotypes were irregularly distributed over time and space in the study area. Egyptian strains of B. melitensis showed MLVA-16 patterns closer to that of Italian strains. Egyptian B. abortus strains isolated from cattle share the same genotype with strains from Portugal and similar to strains from Italy with low genetic diversity. Strains with similar MLVA patterns isolated from different governorates highlight the movement of the pathogen among governorates. Hence, it may also reflect the long endemicity of brucellosis in Egypt with earlier dispersal of types and great local genetic diversity. Open markets may contribute to cross-species transmission and dissemination of the new types nationwide. The presence of West Mediterranean lineages of B. melitensis and relatedness of B. abortus strains from the studied countries is a result of the socio-historical connections among the Mediterranean countries. Transnational eradication of brucellosis in the Mediterranean basin is highly demanded.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-06-22
2020
2020-06-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8374
url https://hdl.handle.net/20.500.14352/8374
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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