Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis

Canine leishmaniosis has a wide range of disease severity from mild (stage I), to severe (stages II-III), or very severe disease (stage IV). The objective of the study was to evaluate and compare serum antibody levels, Leishmania infantum specific IFN- γ production and TLR2 and TLR4 transcripts in n...

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Autores: Sangrà, Sara Montserrat, Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358, Martínez Orellana, Pamela, Solano Gallego, Laia|||0000-0001-8479-4896
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:201569
Acceso en línea:https://ddd.uab.cat/record/201569
https://dx.doi.org/urn:doi:10.3390/vetsci5010031
Access Level:acceso abierto
Palabra clave:Blood parasitemia
Dog
IFN-γ
Leishmania infantum
Papular dermatitis
TLR2
TLR4
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spelling Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosisSangrà, Sara MontserratOrdeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358Martínez Orellana, PamelaSolano Gallego, Laia|||0000-0001-8479-4896Blood parasitemiaDogIFN-γLeishmania infantumPapular dermatitisTLR2TLR4Canine leishmaniosis has a wide range of disease severity from mild (stage I), to severe (stages II-III), or very severe disease (stage IV). The objective of the study was to evaluate and compare serum antibody levels, Leishmania infantum specific IFN- γ production and TLR2 and TLR4 transcripts in non-stimulated blood from dogs with different clinical stages at the time of diagnosis as well as blood parasitemia. Enzyme-Linked ImmunoSorbent Assay (ELISAs) were performed to determine serum antibody levels and IFN- γ production and quantitative polymerase chain reaction (qPCRs) in order to determine blood parasite load and TLR2 and TLR4 transcripts. Older dogs were significantly affected by more severe disease with higher antibody levels and blood parasitemia than dogs with mild disease. IFN- γ production was significantly higher in dogs with stage I disease when compared to dogs with more severe disease. Relative quantification of TLR2 in dogs with mild disease was similar to that of control dogs. On the other hand, TLR2 transcripts were significantly higher in dogs with severe disease as compared with that from control healthy dogs. No differences were found in TLR4 relative quantification between groups. This study demonstrates that dogs with different clinical stages of leishmaniosis present different levels of biological markers indicative of different immune responses. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/201569https://dx.doi.org/urn:doi:10.3390/vetsci5010031reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2015692026-06-06T12:50:31Z
dc.title.none.fl_str_mv Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
title Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
spellingShingle Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
Sangrà, Sara Montserrat
Blood parasitemia
Dog
IFN-γ
Leishmania infantum
Papular dermatitis
TLR2
TLR4
title_short Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
title_full Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
title_fullStr Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
title_full_unstemmed Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
title_sort Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis
dc.creator.none.fl_str_mv Sangrà, Sara Montserrat
Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Martínez Orellana, Pamela
Solano Gallego, Laia|||0000-0001-8479-4896
author Sangrà, Sara Montserrat
author_facet Sangrà, Sara Montserrat
Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Martínez Orellana, Pamela
Solano Gallego, Laia|||0000-0001-8479-4896
author_role author
author2 Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Martínez Orellana, Pamela
Solano Gallego, Laia|||0000-0001-8479-4896
author2_role author
author
author
dc.subject.none.fl_str_mv Blood parasitemia
Dog
IFN-γ
Leishmania infantum
Papular dermatitis
TLR2
TLR4
topic Blood parasitemia
Dog
IFN-γ
Leishmania infantum
Papular dermatitis
TLR2
TLR4
description Canine leishmaniosis has a wide range of disease severity from mild (stage I), to severe (stages II-III), or very severe disease (stage IV). The objective of the study was to evaluate and compare serum antibody levels, Leishmania infantum specific IFN- γ production and TLR2 and TLR4 transcripts in non-stimulated blood from dogs with different clinical stages at the time of diagnosis as well as blood parasitemia. Enzyme-Linked ImmunoSorbent Assay (ELISAs) were performed to determine serum antibody levels and IFN- γ production and quantitative polymerase chain reaction (qPCRs) in order to determine blood parasite load and TLR2 and TLR4 transcripts. Older dogs were significantly affected by more severe disease with higher antibody levels and blood parasitemia than dogs with mild disease. IFN- γ production was significantly higher in dogs with stage I disease when compared to dogs with more severe disease. Relative quantification of TLR2 in dogs with mild disease was similar to that of control dogs. On the other hand, TLR2 transcripts were significantly higher in dogs with severe disease as compared with that from control healthy dogs. No differences were found in TLR4 relative quantification between groups. This study demonstrates that dogs with different clinical stages of leishmaniosis present different levels of biological markers indicative of different immune responses.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/201569
https://dx.doi.org/urn:doi:10.3390/vetsci5010031
url https://ddd.uab.cat/record/201569
https://dx.doi.org/urn:doi:10.3390/vetsci5010031
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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