Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation

Primary graft dysfunction (PGD) remains a major cause of early morbidity and mortality after a heart transplant (HTx). Understanding the donor-related mechanisms involved may help improve organ selection and post-HTx outcomes. This study aimed to explore the association between the donor serum bioma...

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Autores: Herrador, Lorena, González-Costello, José, Niubo Bosch, Jordi, Calatayud Samper, Laura, Maestro Benedicto, Alba, Farrero Torres, Marta, Blasco Peiró, Teresa, Almenar Bonet, Luis, Blázquez Bermejo, Zorba, Garrido Bravo, Iris, Gran Ipiña, Ferran, Grande Trillo, Antonio, Manito, Nicolas, Moreno González, Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/224333
Acceso en línea:https://hdl.handle.net/2445/224333
Access Level:acceso abierto
Palabra clave:Inflamació
Apoptosi
Trasplantament cardíac
Inflammation
Apoptosis
Heart transplantation
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spelling Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart TransplantationHerrador, LorenaGonzález-Costello, JoséNiubo Bosch, JordiCalatayud Samper, LauraMaestro Benedicto, AlbaFarrero Torres, MartaBlasco Peiró, TeresaAlmenar Bonet, LuisBlázquez Bermejo, ZorbaGarrido Bravo, IrisGran Ipiña, FerranGrande Trillo, AntonioManito, NicolasMoreno González, GabrielInflamacióApoptosiTrasplantament cardíacInflammationApoptosisHeart transplantationPrimary graft dysfunction (PGD) remains a major cause of early morbidity and mortality after a heart transplant (HTx). Understanding the donor-related mechanisms involved may help improve organ selection and post-HTx outcomes. This study aimed to explore the association between the donor serum biomarkers of cell death and inflammation and the incidence of PGD and rejection in HTx recipients. We conducted a retrospective, multicenter observational study of brain-dead (DBD) heart donors and corresponding recipients between 2013 and 2019. Donor blood samples were analyzed for inflammatory cytokines, cell death-related proteins, and mitochondrial (mtDNA) and genomic DNA (gDNA). A total of 39 donor-recipient pairs were included. Sixteen recipients developed severe PGD, and five experienced >= 2R cellular rejection. Donors whose recipients developed PGD had significantly lower serum Caspase-3 levels compared to those without PGD (391.6 [101.8-1003.3] vs. 65.3 [40.2-163.3] pg/mL; p = 0.04). A trend toward lower mtDNA/gDNA ratio was also observed in the same group (10.5 [5.4-24.6] vs. 6.5 [3.3-10.7]; p = 0.067). Lower Caspase-3 levels in donor serum were significantly associated with the development of severe PGD in recipients. This may suggest that the sublethal activation of apoptotic pathways in the donor could play a protective role, potentially conditioning the graft to tolerate ischemic injury.MDPI2025202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/224333Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/ijms26199434International Journal of Molecular Sciences, 2025, vol. 26, num. 19, 9434https://doi.org/10.3390/ijms26199434cc-by (c) Herrador, Lorena et al., 2025https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2243332026-05-29T05:05:01Z
dc.title.none.fl_str_mv Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
title Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
spellingShingle Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
Herrador, Lorena
Inflamació
Apoptosi
Trasplantament cardíac
Inflammation
Apoptosis
Heart transplantation
title_short Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
title_full Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
title_fullStr Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
title_full_unstemmed Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
title_sort Caspase-3 in Brain Death Donors Is Associated with Reduced Primary Graft Dysfunction After Heart Transplantation
dc.creator.none.fl_str_mv Herrador, Lorena
González-Costello, José
Niubo Bosch, Jordi
Calatayud Samper, Laura
Maestro Benedicto, Alba
Farrero Torres, Marta
Blasco Peiró, Teresa
Almenar Bonet, Luis
Blázquez Bermejo, Zorba
Garrido Bravo, Iris
Gran Ipiña, Ferran
Grande Trillo, Antonio
Manito, Nicolas
Moreno González, Gabriel
author Herrador, Lorena
author_facet Herrador, Lorena
González-Costello, José
Niubo Bosch, Jordi
Calatayud Samper, Laura
Maestro Benedicto, Alba
Farrero Torres, Marta
Blasco Peiró, Teresa
Almenar Bonet, Luis
Blázquez Bermejo, Zorba
Garrido Bravo, Iris
Gran Ipiña, Ferran
Grande Trillo, Antonio
Manito, Nicolas
Moreno González, Gabriel
author_role author
author2 González-Costello, José
Niubo Bosch, Jordi
Calatayud Samper, Laura
Maestro Benedicto, Alba
Farrero Torres, Marta
Blasco Peiró, Teresa
Almenar Bonet, Luis
Blázquez Bermejo, Zorba
Garrido Bravo, Iris
Gran Ipiña, Ferran
Grande Trillo, Antonio
Manito, Nicolas
Moreno González, Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Inflamació
Apoptosi
Trasplantament cardíac
Inflammation
Apoptosis
Heart transplantation
topic Inflamació
Apoptosi
Trasplantament cardíac
Inflammation
Apoptosis
Heart transplantation
description Primary graft dysfunction (PGD) remains a major cause of early morbidity and mortality after a heart transplant (HTx). Understanding the donor-related mechanisms involved may help improve organ selection and post-HTx outcomes. This study aimed to explore the association between the donor serum biomarkers of cell death and inflammation and the incidence of PGD and rejection in HTx recipients. We conducted a retrospective, multicenter observational study of brain-dead (DBD) heart donors and corresponding recipients between 2013 and 2019. Donor blood samples were analyzed for inflammatory cytokines, cell death-related proteins, and mitochondrial (mtDNA) and genomic DNA (gDNA). A total of 39 donor-recipient pairs were included. Sixteen recipients developed severe PGD, and five experienced >= 2R cellular rejection. Donors whose recipients developed PGD had significantly lower serum Caspase-3 levels compared to those without PGD (391.6 [101.8-1003.3] vs. 65.3 [40.2-163.3] pg/mL; p = 0.04). A trend toward lower mtDNA/gDNA ratio was also observed in the same group (10.5 [5.4-24.6] vs. 6.5 [3.3-10.7]; p = 0.067). Lower Caspase-3 levels in donor serum were significantly associated with the development of severe PGD in recipients. This may suggest that the sublethal activation of apoptotic pathways in the donor could play a protective role, potentially conditioning the graft to tolerate ischemic injury.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/224333
url https://hdl.handle.net/2445/224333
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms26199434
International Journal of Molecular Sciences, 2025, vol. 26, num. 19, 9434
https://doi.org/10.3390/ijms26199434
dc.rights.none.fl_str_mv cc-by (c) Herrador, Lorena et al., 2025
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Herrador, Lorena et al., 2025
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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