Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors
Plant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of Piper nigrum showed anti-Candida activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in Candida albicans, which have not be...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/104561 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/104561 |
| Access Level: | acceso abierto |
| Palabra clave: | 579 615.32 Antifungal activity Candida albicans Mechanism of action Piperine Virulence factors Microbiología (Farmacia) Farmacología (Farmacia) Enfermedades infecciosas 3302.03 Microbiología Industrial 3209.05 Farmacognosia 3205.05 Enfermedades Infecciosas |
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Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factorsBravo Chaucanés, Claudia PatriciaChitiva, Luis CarlosVargas Casanova, YerlyDiaz Santoyo, ValentinaHernández, Andrea XimenaCosta, Geison M.Parra Giraldo, Claudia Marcela579615.32Antifungal activityCandida albicansMechanism of actionPiperineVirulence factorsMicrobiología (Farmacia)Farmacología (Farmacia)Enfermedades infecciosas3302.03 Microbiología Industrial3209.05 Farmacognosia3205.05 Enfermedades InfecciosasPlant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of Piper nigrum showed anti-Candida activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in Candida albicans, which have not been comprehensively understood, were also assessed. Initially, piperine suppressed the hyphal transition in both liquid and solid media, hindered biofilm formation, and resulted in observable cell distortions in scanning electron microscope (SEM) samples, for both fluconazole-sensitive and fluconazole-resistant C. albicans strains. Additionally, the morphogenetic switches triggered by piperine were found to rely on the activity of mutant C. albicans strains. Secondly, piperine treatment increased cell membrane permeability and disrupted mitochondrial membrane potential, as evidenced by propidium iodine and Rhodamine 123 staining, respectively. Moreover, it induced the accumulation of intracellular reactive oxygen species in C. albicans. Synergy was obtained between the piperine and the fluconazole against the fluconazole-sensitive strain. Interestingly, there were no hemolytic effects of piperine, and it resulted in reduced cytotoxicity on fibroblast cells at low concentrations. The results suggest that piperine could have a dual mode of action inhibiting virulence factors and modulating cellular processes, leading to cell death in C. albicans.MDPIUniversidad Complutense de Madrid20232023-11-3020232023-11-30journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/104561reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1045612026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| title |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| spellingShingle |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors Bravo Chaucanés, Claudia Patricia 579 615.32 Antifungal activity Candida albicans Mechanism of action Piperine Virulence factors Microbiología (Farmacia) Farmacología (Farmacia) Enfermedades infecciosas 3302.03 Microbiología Industrial 3209.05 Farmacognosia 3205.05 Enfermedades Infecciosas |
| title_short |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| title_full |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| title_fullStr |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| title_full_unstemmed |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| title_sort |
Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors |
| dc.creator.none.fl_str_mv |
Bravo Chaucanés, Claudia Patricia Chitiva, Luis Carlos Vargas Casanova, Yerly Diaz Santoyo, Valentina Hernández, Andrea Ximena Costa, Geison M. Parra Giraldo, Claudia Marcela |
| author |
Bravo Chaucanés, Claudia Patricia |
| author_facet |
Bravo Chaucanés, Claudia Patricia Chitiva, Luis Carlos Vargas Casanova, Yerly Diaz Santoyo, Valentina Hernández, Andrea Ximena Costa, Geison M. Parra Giraldo, Claudia Marcela |
| author_role |
author |
| author2 |
Chitiva, Luis Carlos Vargas Casanova, Yerly Diaz Santoyo, Valentina Hernández, Andrea Ximena Costa, Geison M. Parra Giraldo, Claudia Marcela |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
579 615.32 Antifungal activity Candida albicans Mechanism of action Piperine Virulence factors Microbiología (Farmacia) Farmacología (Farmacia) Enfermedades infecciosas 3302.03 Microbiología Industrial 3209.05 Farmacognosia 3205.05 Enfermedades Infecciosas |
| topic |
579 615.32 Antifungal activity Candida albicans Mechanism of action Piperine Virulence factors Microbiología (Farmacia) Farmacología (Farmacia) Enfermedades infecciosas 3302.03 Microbiología Industrial 3209.05 Farmacognosia 3205.05 Enfermedades Infecciosas |
| description |
Plant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of Piper nigrum showed anti-Candida activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in Candida albicans, which have not been comprehensively understood, were also assessed. Initially, piperine suppressed the hyphal transition in both liquid and solid media, hindered biofilm formation, and resulted in observable cell distortions in scanning electron microscope (SEM) samples, for both fluconazole-sensitive and fluconazole-resistant C. albicans strains. Additionally, the morphogenetic switches triggered by piperine were found to rely on the activity of mutant C. albicans strains. Secondly, piperine treatment increased cell membrane permeability and disrupted mitochondrial membrane potential, as evidenced by propidium iodine and Rhodamine 123 staining, respectively. Moreover, it induced the accumulation of intracellular reactive oxygen species in C. albicans. Synergy was obtained between the piperine and the fluconazole against the fluconazole-sensitive strain. Interestingly, there were no hemolytic effects of piperine, and it resulted in reduced cytotoxicity on fibroblast cells at low concentrations. The results suggest that piperine could have a dual mode of action inhibiting virulence factors and modulating cellular processes, leading to cell death in C. albicans. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023-11-30 2023 2023-11-30 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/104561 |
| url |
https://hdl.handle.net/20.500.14352/104561 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
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Universidad Complutense de Madrid (UCM) |
| reponame_str |
Docta Complutense |
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Docta Complutense |
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1869412498189320192 |
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15,301603 |