Caracterización molecular de los fitoplasmas del grupo Apple proliferation asociados a los síntomas de escoba de bruja en Retama

[EN]Since 1999 witches' broom and decline diseases have been observed in Spartium junceum, bom spontaneous and ornamental shrubs located in urban areas, in all the seaside regions of Catalonia (Northeastern Spain). The symptoms may affect the whole plant or few branches. The witches' broom...

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Detalles Bibliográficos
Autores: Martín, María P., Torres, Ester, Botti, S., Bertaccini, Assunta
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/45694
Acceso en línea:http://hdl.handle.net/10261/45694
Access Level:acceso abierto
Palabra clave:Spartium junceum
grupo 16 SrX
filogenia
PCR
RFLP
secuenciación
Descripción
Sumario:[EN]Since 1999 witches' broom and decline diseases have been observed in Spartium junceum, bom spontaneous and ornamental shrubs located in urban areas, in all the seaside regions of Catalonia (Northeastern Spain). The symptoms may affect the whole plant or few branches. The witches' broom developed from axillar buds is the most important symptom. Affected plants end up turning very dry, and eventually die in a few years. These symptoms have been recently associated in Italy to the presence of apple proliferation phytoplasma group. In this work the molecular methods (nested PCR, RFLP and sequencing) that allow the characterization of the phytoplasmas detected in symptomatic Spanish Broom shrubs from Catalunya are described. The phytoplasmas detected are molecularly undistinguishable from others of the 16SrX group (apple proliferation),subgroup D.