Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea

Free and glycosylated sterols are both structural components of the plasma membrane that regulate their biophysical properties and consequently different plasma membrane-associated processes such as plant adaptation to stress or signaling. Several reports relate changes in glycosylated sterols level...

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Autores: Castillo, Nídia, Pastor, Victoria, Chávez, Ángel, Arró i Plans, Montserrat, Boronat i Margosa, Albert, Flors, Víctor, Ferrer i Prats, Albert, Altabella Artigas, Teresa
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/148005
Acesso em linha:https://hdl.handle.net/2445/148005
Access Level:acceso abierto
Palavra-chave:Arabidopsis
Fongs patògens
Pathogenic fungi
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spelling Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinereaCastillo, NídiaPastor, VictoriaChávez, ÁngelArró i Plans, MontserratBoronat i Margosa, AlbertFlors, VíctorFerrer i Prats, AlbertAltabella Artigas, TeresaArabidopsisFongs patògensArabidopsisPathogenic fungiFree and glycosylated sterols are both structural components of the plasma membrane that regulate their biophysical properties and consequently different plasma membrane-associated processes such as plant adaptation to stress or signaling. Several reports relate changes in glycosylated sterols levels with the plant response to abiotic stress, but the information about the role of these compounds in the response to biotic stress is scarce. In this work, we have studied the response to the necrotrophic fungus Botrytis cinerea in an Arabidopsis mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases (UGT80A2 and UGT80B1) reported in this plant. This mutant exhibits enhanced resistance against B. cinerea when compared to wild-type plants, which correlates with increased levels of jasmonic acid (JA) and up-regulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Upon B. cinerea infection, the ugt80A2;B1 double mutant also accumulates higher levels of camalexin, the major Arabidopsis phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also up-regulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants. Altogether, the results of this study show that glycosylated sterols play an important role in the regulation of Arabidopsis response to B. cinerea infection and suggest that this occurs through signaling pathways involving the canonical stress-hormone JA and the tryptophan-derived secondary metabolites camalexin and possibly also indole glucosinolatesFrontiers Media2020202020192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/148005Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fpls.2019.01162Frontiers in Plant Science, 2019, vol. 10, p. 1162https://doi.org/10.3389/fpls.2019.01162cc-by (c) Castillo, Nídia et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1480052026-05-29T05:05:01Z
dc.title.none.fl_str_mv Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
title Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
spellingShingle Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
Castillo, Nídia
Arabidopsis
Fongs patògens
Arabidopsis
Pathogenic fungi
title_short Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
title_full Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
title_fullStr Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
title_full_unstemmed Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
title_sort Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
dc.creator.none.fl_str_mv Castillo, Nídia
Pastor, Victoria
Chávez, Ángel
Arró i Plans, Montserrat
Boronat i Margosa, Albert
Flors, Víctor
Ferrer i Prats, Albert
Altabella Artigas, Teresa
author Castillo, Nídia
author_facet Castillo, Nídia
Pastor, Victoria
Chávez, Ángel
Arró i Plans, Montserrat
Boronat i Margosa, Albert
Flors, Víctor
Ferrer i Prats, Albert
Altabella Artigas, Teresa
author_role author
author2 Pastor, Victoria
Chávez, Ángel
Arró i Plans, Montserrat
Boronat i Margosa, Albert
Flors, Víctor
Ferrer i Prats, Albert
Altabella Artigas, Teresa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Fongs patògens
Arabidopsis
Pathogenic fungi
topic Arabidopsis
Fongs patògens
Arabidopsis
Pathogenic fungi
description Free and glycosylated sterols are both structural components of the plasma membrane that regulate their biophysical properties and consequently different plasma membrane-associated processes such as plant adaptation to stress or signaling. Several reports relate changes in glycosylated sterols levels with the plant response to abiotic stress, but the information about the role of these compounds in the response to biotic stress is scarce. In this work, we have studied the response to the necrotrophic fungus Botrytis cinerea in an Arabidopsis mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases (UGT80A2 and UGT80B1) reported in this plant. This mutant exhibits enhanced resistance against B. cinerea when compared to wild-type plants, which correlates with increased levels of jasmonic acid (JA) and up-regulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Upon B. cinerea infection, the ugt80A2;B1 double mutant also accumulates higher levels of camalexin, the major Arabidopsis phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also up-regulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants. Altogether, the results of this study show that glycosylated sterols play an important role in the regulation of Arabidopsis response to B. cinerea infection and suggest that this occurs through signaling pathways involving the canonical stress-hormone JA and the tryptophan-derived secondary metabolites camalexin and possibly also indole glucosinolates
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/148005
url https://hdl.handle.net/2445/148005
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fpls.2019.01162
Frontiers in Plant Science, 2019, vol. 10, p. 1162
https://doi.org/10.3389/fpls.2019.01162
dc.rights.none.fl_str_mv cc-by (c) Castillo, Nídia et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Castillo, Nídia et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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