Fluorescence-based biosensors for West Nile virus detection [Dataset]
Flaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishme...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/412756 |
| Acceso en línea: | http://hdl.handle.net/10261/412756 |
| Access Level: | acceso abierto |
| Palabra clave: | Arboviruses Flaviviruses West Nile virus Biosensors Diagnosis Antivirals |
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Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| title |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| spellingShingle |
Fluorescence-based biosensors for West Nile virus detection [Dataset] Blázquez, Ana-Belén Arboviruses Flaviviruses West Nile virus Biosensors Diagnosis Antivirals |
| title_short |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| title_full |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| title_fullStr |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| title_full_unstemmed |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| title_sort |
Fluorescence-based biosensors for West Nile virus detection [Dataset] |
| dc.creator.none.fl_str_mv |
Blázquez, Ana-Belén Saiz, Juan Carlos Herrero, Laura Sánchez-Céspedes, Javier Vázquez, Ana Cisneros, José Miguel Martín-Acebes, M. A. Jiménez de Oya, Nereida |
| author |
Blázquez, Ana-Belén |
| author_facet |
Blázquez, Ana-Belén Saiz, Juan Carlos Herrero, Laura Sánchez-Céspedes, Javier Vázquez, Ana Cisneros, José Miguel Martín-Acebes, M. A. Jiménez de Oya, Nereida |
| author_role |
author |
| author2 |
Saiz, Juan Carlos Herrero, Laura Sánchez-Céspedes, Javier Vázquez, Ana Cisneros, José Miguel Martín-Acebes, M. A. Jiménez de Oya, Nereida |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
European Commission Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid Instituto de Salud Carlos III CSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global) Blázquez, Ana-Belén [0000-0002-0847-0977] Saiz, Juan Carlos [0000-0001-8269-5544] Sánchez-Céspedes, Javier [0000-0003-2707-1979] Vázquez, Ana [0000-0002-9872-3316] Cisneros, José Miguel [0000-0001-5001-672X] Martín-Acebes, M. A. [0000-0001-6015-3613] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Arboviruses Flaviviruses West Nile virus Biosensors Diagnosis Antivirals |
| topic |
Arboviruses Flaviviruses West Nile virus Biosensors Diagnosis Antivirals |
| description |
Flaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishment of their vectors in new geographic areas, thus promoting epidemic outbreaks and facilitating these viruses to become endemic in these areas. This is the case of the West Nile virus (WNV) in Europe, which affects its natural bird host populations and also accidental hosts such as humans and horses. Flaviviruses are antigenically related, which induces cross-reactivity, making their serological diagnosis difficult, especially in areas where several flaviviruses co-circulate. Here, we have developed WNV biosensors in which the enzymatic activity of the viral protease, expressed during infection, allows its detection using fluorescence-based techniques. These biosensors carry WNV-specific protease cleavage sites and show high specificity for the detection of both lineages 1 and 2, with limits of detection (LODs) ranging from 0.001-0.0001 MOI at 48 hours post-infection (h.p.i.). These LODs are even lower at 72 h.p.i. reaching as low as an MOI of 2.5 × 10−6. They are also capable of detecting, although with lower sensitivity, other flaviviruses such as dengue, Zika, and Usutu viruses, without showing reactivity against unrelated viruses. These biosensors have been validated in viral neutralization assays with sera from infected mice and humans, as well as in antiviral screening, with results comparable to those of currently used systems, showing significant potential as clinical and laboratory tools. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2026 2026 |
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info:eu-repo/semantics/dataset http://purl.org/coar/resource_type/c_ddb1 |
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dataset |
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http://hdl.handle.net/10261/412756 |
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http://hdl.handle.net/10261/412756 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119195RJ-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137372OR-C21 TEC-2024/BIO-66/SALAINDEC-CM Blázquez, Ana-Belén; Saiz, Juan Carlos; Herrero, Laura; Sánchez-Céspedes, Javier; Vázquez, Ana; Cisneros, José Miguel; Martín-Acebes, M. A.; Jiménez de Oya, Nereida. Fluorescence-based biosensors for West Nile virus detection. https://doi.org/10.1080/21505594.2025.2597663. http://hdl.handle.net/10261/412752 Jiménez de Oya, Nereida; 2025; Fluorescence-based biosensors for West Nile virus detection [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.30619145 https://doi.org/10.6084/m9.figshare.30797753 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Fluorescence-based biosensors for West Nile virus detection [Dataset]Blázquez, Ana-BelénSaiz, Juan CarlosHerrero, LauraSánchez-Céspedes, JavierVázquez, AnaCisneros, José MiguelMartín-Acebes, M. A.Jiménez de Oya, NereidaArbovirusesFlavivirusesWest Nile virusBiosensorsDiagnosisAntiviralsFlaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishment of their vectors in new geographic areas, thus promoting epidemic outbreaks and facilitating these viruses to become endemic in these areas. This is the case of the West Nile virus (WNV) in Europe, which affects its natural bird host populations and also accidental hosts such as humans and horses. Flaviviruses are antigenically related, which induces cross-reactivity, making their serological diagnosis difficult, especially in areas where several flaviviruses co-circulate. Here, we have developed WNV biosensors in which the enzymatic activity of the viral protease, expressed during infection, allows its detection using fluorescence-based techniques. These biosensors carry WNV-specific protease cleavage sites and show high specificity for the detection of both lineages 1 and 2, with limits of detection (LODs) ranging from 0.001-0.0001 MOI at 48 hours post-infection (h.p.i.). These LODs are even lower at 72 h.p.i. reaching as low as an MOI of 2.5 × 10−6. They are also capable of detecting, although with lower sensitivity, other flaviviruses such as dengue, Zika, and Usutu viruses, without showing reactivity against unrelated viruses. These biosensors have been validated in viral neutralization assays with sera from infected mice and humans, as well as in antiviral screening, with results comparable to those of currently used systems, showing significant potential as clinical and laboratory tools.This work was supported by the Spanish Ministry of Science and Innovation AEI/10.13039/501100011033 under Grants [PID2020-119195RJ-I00] (to NJO) and [PID2022-137372OR-C21] (to MAMA), by Comunidad de Madrid under grant [TEC-2024/BIO-66/SALAINDEC-CM] (to MAMA), by the Instituto de Salud Carlos III Project PI19CIII_00014, and by the European Commission—NextGenerationEU through [CSIC’s Global Health Platform] (PTI Salud Global). The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.Peer reviewedFigshareEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Comunidad de MadridInstituto de Salud Carlos IIICSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global)Blázquez, Ana-Belén [0000-0002-0847-0977]Saiz, Juan Carlos [0000-0001-8269-5544]Sánchez-Céspedes, Javier [0000-0003-2707-1979]Vázquez, Ana [0000-0002-9872-3316]Cisneros, José Miguel [0000-0001-5001-672X]Martín-Acebes, M. A. [0000-0001-6015-3613]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/wordperfect5.1text/csvhttp://hdl.handle.net/10261/412756reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119195RJ-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137372OR-C21TEC-2024/BIO-66/SALAINDEC-CMBlázquez, Ana-Belén; Saiz, Juan Carlos; Herrero, Laura; Sánchez-Céspedes, Javier; Vázquez, Ana; Cisneros, José Miguel; Martín-Acebes, M. A.; Jiménez de Oya, Nereida. Fluorescence-based biosensors for West Nile virus detection. https://doi.org/10.1080/21505594.2025.2597663. http://hdl.handle.net/10261/412752Jiménez de Oya, Nereida; 2025; Fluorescence-based biosensors for West Nile virus detection [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.30619145https://doi.org/10.6084/m9.figshare.30797753Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4127562026-05-22T06:33:51Z |
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15,812429 |