Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

Due to the protogynous dichogamy of cherimoya and to the absence of proper pollinating vectors, hand-pollination with fresh pollen is a common practice for cherimoya commercial production. In order to optimize the process of hand-pollination, in this work we have studied the conservation of cherimoy...

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Autores: Lora, Jorge, Pérez de Oteyza, María de los Angeles, Fuentetaja, Pilar, Hormaza Urroz, José Ignacio
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/5588
Acceso en línea:http://hdl.handle.net/10261/5588
Access Level:acceso abierto
Palabra clave:Cherimoya
Annonaceae
Cryopreservation
In vitro pollen germination
Pollen viability
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spelling Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollenLora, JorgePérez de Oteyza, María de los AngelesFuentetaja, PilarHormaza Urroz, José IgnacioCherimoyaAnnonaceaeCryopreservationIn vitro pollen germinationPollen viabilityDue to the protogynous dichogamy of cherimoya and to the absence of proper pollinating vectors, hand-pollination with fresh pollen is a common practice for cherimoya commercial production. In order to optimize the process of hand-pollination, in this work we have studied the conservation of cherimoya pollen at –20, -80 and –196ºC for up to three months. In vitro pollen germination of fresh pollen was 57.1% and it was progressively reduced with conservation time at the three temperatures studied reaching a minimum after three months of storage of 10.4 %, 14.2% and 13.6% at –20, -80 and –196ºC, respectively. Differences in germination among temperatures were only significant during the first two weeks of storage. Field pollinations with pollen stored for up to three months at the three temperatures show no yield differences compared to pollinations performed with fresh pollen. The results indicate that pollen collected and stored at sub-zero temperatures at the beginning of the cherimoya blooming season can be used along the whole blooming season avoiding the need of collecting fresh pollen daily.Financial support for this work was provided by MCYT (Project Grant AGL2003-05318-C02-01).Peer reviewedElsevier200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_650178336 bytesapplication/mswordhttp://hdl.handle.net/10261/5588reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/55882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
title Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
spellingShingle Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
Lora, Jorge
Cherimoya
Annonaceae
Cryopreservation
In vitro pollen germination
Pollen viability
title_short Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
title_full Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
title_fullStr Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
title_full_unstemmed Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
title_sort Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen
dc.creator.none.fl_str_mv Lora, Jorge
Pérez de Oteyza, María de los Angeles
Fuentetaja, Pilar
Hormaza Urroz, José Ignacio
author Lora, Jorge
author_facet Lora, Jorge
Pérez de Oteyza, María de los Angeles
Fuentetaja, Pilar
Hormaza Urroz, José Ignacio
author_role author
author2 Pérez de Oteyza, María de los Angeles
Fuentetaja, Pilar
Hormaza Urroz, José Ignacio
author2_role author
author
author
dc.subject.none.fl_str_mv Cherimoya
Annonaceae
Cryopreservation
In vitro pollen germination
Pollen viability
topic Cherimoya
Annonaceae
Cryopreservation
In vitro pollen germination
Pollen viability
description Due to the protogynous dichogamy of cherimoya and to the absence of proper pollinating vectors, hand-pollination with fresh pollen is a common practice for cherimoya commercial production. In order to optimize the process of hand-pollination, in this work we have studied the conservation of cherimoya pollen at –20, -80 and –196ºC for up to three months. In vitro pollen germination of fresh pollen was 57.1% and it was progressively reduced with conservation time at the three temperatures studied reaching a minimum after three months of storage of 10.4 %, 14.2% and 13.6% at –20, -80 and –196ºC, respectively. Differences in germination among temperatures were only significant during the first two weeks of storage. Field pollinations with pollen stored for up to three months at the three temperatures show no yield differences compared to pollinations performed with fresh pollen. The results indicate that pollen collected and stored at sub-zero temperatures at the beginning of the cherimoya blooming season can be used along the whole blooming season avoiding the need of collecting fresh pollen daily.
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/5588
url http://hdl.handle.net/10261/5588
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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