MoS2 nanosheets for improving analytical performance of lactate biosensors

In this paper, a new 2D lactate electrochemical biosensor was developed. It was based on the incorporation of molybdenum disulfide (MoS2) nanosheets, obtained by an exfoliation method, onto the surface of a glassy carbon (GC) electrode, together with lactate oxidase enzyme (LOx). For the sensor cons...

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Detalles Bibliográficos
Autores: Parra Alfambra, Ana María, Casero Junquera, María Elena, Quintana Mani, María del Carmen, Pozo Vázquez, María del, Petit Domínguez, María Dolores, Vázquez, Luis
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710246
Acceso en línea:http://hdl.handle.net/10486/710246
https://dx.doi.org/10.1016/j.snb.2018.07.124
Access Level:acceso abierto
Palabra clave:Atomic force microscopy
Cyclic voltammetry
Electrochemical biosensor
Electrochemical impedance spectroscopy
Lactate oxidase
Molybdenum disulfide
Química
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spelling MoS2 nanosheets for improving analytical performance of lactate biosensorsParra Alfambra, Ana MaríaCasero Junquera, María ElenaQuintana Mani, María del CarmenPozo Vázquez, María delPetit Domínguez, María DoloresVázquez, LuisAtomic force microscopyCyclic voltammetryElectrochemical biosensorElectrochemical impedance spectroscopyLactate oxidaseMolybdenum disulfideQuímicaIn this paper, a new 2D lactate electrochemical biosensor was developed. It was based on the incorporation of molybdenum disulfide (MoS2) nanosheets, obtained by an exfoliation method, onto the surface of a glassy carbon (GC) electrode, together with lactate oxidase enzyme (LOx). For the sensor construction, conditions regarding the exfoliation solvent type, as well as both the size and amount of MoS2, were optimized. The biosensor platform (GC/MoS2/LOx) was topographically characterized by atomic force microscopy (AFM) and the charge transfer process occurring at the electrode interface was studied by electrochemical impedance spectroscopy (EIS). The GC/MoS2/LOx biosensor was applied to the determination of lactate in presence of hydroxymethylferrocene (HMF) as a redox mediator. Electrocatalytic effect of the system MoS2/LOx was evaluated by comparing the cyclic voltammetric biosensor response with those obtained for biosensors incorporating only one of the components (MoS2 or LOx) onto the electrode surface. Biosensors containing both components exhibit the best electrocatalytic response. From the calibration curve obtained at +0.30 V, the following analytical parameters were obtained: linear concentration range from 0.056 to 0.77 mM, high sensitivity (6.2 μA mM−1), good detection limit (17 μM) and reproducibility (RSD = 4.7%)The authors would like to thank Ministerio de Economía, Industria y Competitividad (MAT2017-85089-C2-1-R, MAT2017-85089-C2-2-R) and the Comunidad Autónoma de Madrid (S2013/MIT-3029, NANOAVANSENS) for financial supportElsevierDepartamento de Química Analítica y Análisis InstrumentalFacultad de Ciencias20182018-07-26research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710246https://dx.doi.org/10.1016/j.snb.2018.07.124reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7102462026-06-23T12:46:27Z
dc.title.none.fl_str_mv MoS2 nanosheets for improving analytical performance of lactate biosensors
title MoS2 nanosheets for improving analytical performance of lactate biosensors
spellingShingle MoS2 nanosheets for improving analytical performance of lactate biosensors
Parra Alfambra, Ana María
Atomic force microscopy
Cyclic voltammetry
Electrochemical biosensor
Electrochemical impedance spectroscopy
Lactate oxidase
Molybdenum disulfide
Química
title_short MoS2 nanosheets for improving analytical performance of lactate biosensors
title_full MoS2 nanosheets for improving analytical performance of lactate biosensors
title_fullStr MoS2 nanosheets for improving analytical performance of lactate biosensors
title_full_unstemmed MoS2 nanosheets for improving analytical performance of lactate biosensors
title_sort MoS2 nanosheets for improving analytical performance of lactate biosensors
dc.creator.none.fl_str_mv Parra Alfambra, Ana María
Casero Junquera, María Elena
Quintana Mani, María del Carmen
Pozo Vázquez, María del
Petit Domínguez, María Dolores
Vázquez, Luis
author Parra Alfambra, Ana María
author_facet Parra Alfambra, Ana María
Casero Junquera, María Elena
Quintana Mani, María del Carmen
Pozo Vázquez, María del
Petit Domínguez, María Dolores
Vázquez, Luis
author_role author
author2 Casero Junquera, María Elena
Quintana Mani, María del Carmen
Pozo Vázquez, María del
Petit Domínguez, María Dolores
Vázquez, Luis
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Analítica y Análisis Instrumental
Facultad de Ciencias
dc.subject.none.fl_str_mv Atomic force microscopy
Cyclic voltammetry
Electrochemical biosensor
Electrochemical impedance spectroscopy
Lactate oxidase
Molybdenum disulfide
Química
topic Atomic force microscopy
Cyclic voltammetry
Electrochemical biosensor
Electrochemical impedance spectroscopy
Lactate oxidase
Molybdenum disulfide
Química
description In this paper, a new 2D lactate electrochemical biosensor was developed. It was based on the incorporation of molybdenum disulfide (MoS2) nanosheets, obtained by an exfoliation method, onto the surface of a glassy carbon (GC) electrode, together with lactate oxidase enzyme (LOx). For the sensor construction, conditions regarding the exfoliation solvent type, as well as both the size and amount of MoS2, were optimized. The biosensor platform (GC/MoS2/LOx) was topographically characterized by atomic force microscopy (AFM) and the charge transfer process occurring at the electrode interface was studied by electrochemical impedance spectroscopy (EIS). The GC/MoS2/LOx biosensor was applied to the determination of lactate in presence of hydroxymethylferrocene (HMF) as a redox mediator. Electrocatalytic effect of the system MoS2/LOx was evaluated by comparing the cyclic voltammetric biosensor response with those obtained for biosensors incorporating only one of the components (MoS2 or LOx) onto the electrode surface. Biosensors containing both components exhibit the best electrocatalytic response. From the calibration curve obtained at +0.30 V, the following analytical parameters were obtained: linear concentration range from 0.056 to 0.77 mM, high sensitivity (6.2 μA mM−1), good detection limit (17 μM) and reproducibility (RSD = 4.7%)
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-07-26
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710246
https://dx.doi.org/10.1016/j.snb.2018.07.124
url http://hdl.handle.net/10486/710246
https://dx.doi.org/10.1016/j.snb.2018.07.124
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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