Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation

15 pages, 8 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)

Detalhes bibliográficos
Autores: Librán-Pérez, Marta, Pereiro, Patricia, Figueras Huerta, Antonio, Novoa, Beatriz
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/274804
Acesso em linha:http://hdl.handle.net/10261/274804
Access Level:acceso abierto
Palavra-chave:Turbot
Aeromonas salmonicida
RNA-Seq
Leptin
Immunometabolism
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spelling Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism RegulationLibrán-Pérez, MartaPereiro, PatriciaFigueras Huerta, AntonioNovoa, BeatrizTurbotAeromonas salmonicidaRNA-SeqLeptinImmunometabolism15 pages, 8 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a disease affecting numerous fish species worldwide. It is a highly pathogenic bacterium for turbot, whose farming production represents an important economic activity in several European countries and China. To better understand the response of this organism to A. salmonicida, we conducted RNA-Seq analysis of the head kidney from experimentally infected and uninfected turbot juveniles at 24 hours post-infection (hpi). As expected, among the differentially expressed genes (DEGs) between infected and uninfected fish, we observed the modulation of a multitude of immune-related genes but also a high representation of genes linked to metabolism. Interestingly, one of the most upregulated genes was that encoding the hormone leptin. Leptin is a multifunctional hormone/cytokine that has been shown to play roles in the immune system, stress response, food intake, metabolism and energy balance. We used recombinant human leptin to elucidate its role during infection with A. salmonicida in turbot (anorexigenic activity, ability to modulate metabolism and the immune response, and its effect on survival and bacterial load during infection). We found that the intraperitoneal administration of leptin was able to alter the response to the bacteria at the immune level, but especially at the metabolic level, which resulted in a higher survival rate without affecting the bacterial load. Based on this, we hypothesized that leptin could offer great potential as a therapeutic treatment during furunculosis outbreaks by reducing the impact of sepsis. Our results reveal the complex interplay between bacterial activity and the regulation of food intake, metabolism and inflammationOur laboratory is funded by projects PID2020-119532RB-I00 from Ministerio de Ciencia e Innovación, MetDisFish from Ministerio de Agricultura, Pesca y Alimentación (MAPA) and European Maritime, Fisheries and Aquaculture Fund (EMFAF), 0474_BLUEBIOLAB from EU FEDER Programa Interreg España-Portugal and IN607B 2019/01 from Consellerıa de Economıa, Emprego e Industria (GAIN), Xunta de Galicia. ML-P and PP wish to thank the Ministerio de Ciencia e Innovación and Axencia Galega de Innovación (GAIN, Xunta de Galicia), respectively, for their postdoctoral contracts (IN606B-2018/010; IN606B-2018/010). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI)Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Ministerio de Agricultura, Pesca y Alimentación (España)Xunta de GaliciaEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/274804reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119532RB-I00The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: NCBI [accession: PRJNA810312]https://doi.org/10.3389/fmars.2022.888115Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2748042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
title Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
spellingShingle Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
Librán-Pérez, Marta
Turbot
Aeromonas salmonicida
RNA-Seq
Leptin
Immunometabolism
title_short Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
title_full Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
title_fullStr Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
title_full_unstemmed Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
title_sort Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected with Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation
dc.creator.none.fl_str_mv Librán-Pérez, Marta
Pereiro, Patricia
Figueras Huerta, Antonio
Novoa, Beatriz
author Librán-Pérez, Marta
author_facet Librán-Pérez, Marta
Pereiro, Patricia
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Pereiro, Patricia
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Agricultura, Pesca y Alimentación (España)
Xunta de Galicia
European Commission
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Turbot
Aeromonas salmonicida
RNA-Seq
Leptin
Immunometabolism
topic Turbot
Aeromonas salmonicida
RNA-Seq
Leptin
Immunometabolism
description 15 pages, 8 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/274804
url http://hdl.handle.net/10261/274804
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119532RB-I00
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: NCBI [accession: PRJNA810312]
https://doi.org/10.3389/fmars.2022.888115
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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