Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi

Termites are important ecosystem engineers and play key roles in modulating microbial communities within and outside their mounds. Microbial diversity within termite mounds is generally lower than surrounding soils, due to termite-associated antimicrobial compounds and active sanitary behaviours. Mi...

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Autores: Bodawatta, K.H., Maccario, Lorrie, Peereboom, Nils, Conlon, Benjamin H., Li, Guangshuo, Plaszkó, Tamás, Vinagre-Izquierdo, Celia, Jønsson, K.A., Vesala, Risto M., Beer, Z. Wilhelm de, Priemé, Anders, Poulsen, Michael
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/387236
Acceso en línea:http://hdl.handle.net/10261/387236
https://api.elsevier.com/content/abstract/scopus_id/85209911301
Access Level:acceso abierto
Palabra clave:Bacteria
Fungi
ICP-MS
MiSeq amplicon sequencing
Symbioses
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spelling Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungiBodawatta, K.H.Maccario, LorriePeereboom, NilsConlon, Benjamin H.Li, GuangshuoPlaszkó, TamásVinagre-Izquierdo, CeliaJønsson, K.A.Vesala, Risto M.Beer, Z. Wilhelm dePriemé, AndersPoulsen, MichaelBacteriaFungiICP-MSMiSeq amplicon sequencingSymbiosesTermites are important ecosystem engineers and play key roles in modulating microbial communities within and outside their mounds. Microbial diversity within termite mounds is generally lower than surrounding soils, due to termite-associated antimicrobial compounds and active sanitary behaviours. Microbial symbionts of termites can also influence the microbial landscape, by inhibiting or out-competing other microbes. Certain members of the arid habitat fungal genus Podaxis (Agaricomycetes; Agaricaceae) are symbiotic with savannah specialist grass-cutting termites, and have the potential to influence mound-associated microbiomes. To test this, we characterized fungal (ITS2) and bacterial (16S rRNA) communities within and outside 49 Trinervitermes trinervoides mounds with and without Podaxis fruiting bodies across a 1000 km transect in South Africa. We predicted that Podaxis would be a dominant member of the fungal communities in mounds and negatively impact microbial diversity. Further, we explored how environmental variables shaped microbial communities, including whether soil elemental composition affected Podaxis presence. As expected, we observed less diverse fungal communities, but not bacterial communities, within than outside mounds, while microbial communities differed by sampling regions and mound compartments. Podaxis sequences were present in 48 out of 49 mounds in low relative abundances, and neither fruiting body presence nor sequence abundance were associated with microbial diversity or composition. There was, however, an overall association between the presence of Podaxis fruiting bodies and elemental composition, with different elements displaying varying associations depending on geographic region. Both environmental variables and soil elements were associated with fungal and bacterial taxa, indicating that they are key drivers of microbial community composition. Taken together, our findings suggest that microbial landscapes in termite mounds are not strongly influenced by Podaxis but mainly driven by termite filtering and regional abiotic variables and elemental compositions.This work was funded by The Danish Council for Independent Research (DFF – 7014-00178) to MP. AP and TP were supported by the Danish National Research Foundation (Center for Volatile Interactions - VOLT, DNRF168). GL was supported by a PhD scholarship from the China Scholarship Council.Peer reviewedElsevier BVDanish Council for Independent ResearchDanish National Research FoundationChina Scholarship CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/387236https://api.elsevier.com/content/abstract/scopus_id/85209911301reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.scitotenv.2024.177015Bodawatta, K.; 2024; Datasets and scripts of "Mound compartments and soil properties, but not symbiotic Podaxis fungi, drive microbial landscapes in Trinervitermes trinervoides termite colonies" [Dataset]; Zenodo; https://doi.org/10.5281/zenodo.13895044https://doi.org/10.1016/j.scitotenv.2024.177015Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3872362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
title Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
spellingShingle Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
Bodawatta, K.H.
Bacteria
Fungi
ICP-MS
MiSeq amplicon sequencing
Symbioses
title_short Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
title_full Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
title_fullStr Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
title_full_unstemmed Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
title_sort Microbial landscapes in Trinervitermes trinervoides termite colonies are affected by mound compartments and soil properties but not by symbiotic Podaxis fungi
dc.creator.none.fl_str_mv Bodawatta, K.H.
Maccario, Lorrie
Peereboom, Nils
Conlon, Benjamin H.
Li, Guangshuo
Plaszkó, Tamás
Vinagre-Izquierdo, Celia
Jønsson, K.A.
Vesala, Risto M.
Beer, Z. Wilhelm de
Priemé, Anders
Poulsen, Michael
author Bodawatta, K.H.
author_facet Bodawatta, K.H.
Maccario, Lorrie
Peereboom, Nils
Conlon, Benjamin H.
Li, Guangshuo
Plaszkó, Tamás
Vinagre-Izquierdo, Celia
Jønsson, K.A.
Vesala, Risto M.
Beer, Z. Wilhelm de
Priemé, Anders
Poulsen, Michael
author_role author
author2 Maccario, Lorrie
Peereboom, Nils
Conlon, Benjamin H.
Li, Guangshuo
Plaszkó, Tamás
Vinagre-Izquierdo, Celia
Jønsson, K.A.
Vesala, Risto M.
Beer, Z. Wilhelm de
Priemé, Anders
Poulsen, Michael
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Danish Council for Independent Research
Danish National Research Foundation
China Scholarship Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacteria
Fungi
ICP-MS
MiSeq amplicon sequencing
Symbioses
topic Bacteria
Fungi
ICP-MS
MiSeq amplicon sequencing
Symbioses
description Termites are important ecosystem engineers and play key roles in modulating microbial communities within and outside their mounds. Microbial diversity within termite mounds is generally lower than surrounding soils, due to termite-associated antimicrobial compounds and active sanitary behaviours. Microbial symbionts of termites can also influence the microbial landscape, by inhibiting or out-competing other microbes. Certain members of the arid habitat fungal genus Podaxis (Agaricomycetes; Agaricaceae) are symbiotic with savannah specialist grass-cutting termites, and have the potential to influence mound-associated microbiomes. To test this, we characterized fungal (ITS2) and bacterial (16S rRNA) communities within and outside 49 Trinervitermes trinervoides mounds with and without Podaxis fruiting bodies across a 1000 km transect in South Africa. We predicted that Podaxis would be a dominant member of the fungal communities in mounds and negatively impact microbial diversity. Further, we explored how environmental variables shaped microbial communities, including whether soil elemental composition affected Podaxis presence. As expected, we observed less diverse fungal communities, but not bacterial communities, within than outside mounds, while microbial communities differed by sampling regions and mound compartments. Podaxis sequences were present in 48 out of 49 mounds in low relative abundances, and neither fruiting body presence nor sequence abundance were associated with microbial diversity or composition. There was, however, an overall association between the presence of Podaxis fruiting bodies and elemental composition, with different elements displaying varying associations depending on geographic region. Both environmental variables and soil elements were associated with fungal and bacterial taxa, indicating that they are key drivers of microbial community composition. Taken together, our findings suggest that microbial landscapes in termite mounds are not strongly influenced by Podaxis but mainly driven by termite filtering and regional abiotic variables and elemental compositions.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/387236
https://api.elsevier.com/content/abstract/scopus_id/85209911301
url http://hdl.handle.net/10261/387236
https://api.elsevier.com/content/abstract/scopus_id/85209911301
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.scitotenv.2024.177015
Bodawatta, K.; 2024; Datasets and scripts of "Mound compartments and soil properties, but not symbiotic Podaxis fungi, drive microbial landscapes in Trinervitermes trinervoides termite colonies" [Dataset]; Zenodo; https://doi.org/10.5281/zenodo.13895044
https://doi.org/10.1016/j.scitotenv.2024.177015
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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