Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery

Stimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D...

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Authors: García, Mónica C., Naitlho, Nabila, Calderón Montaño, José Manuel, Drago, Estrella, Rueda Rueda, Manuela, Longhi, Marcela, Rabasco Álvarez, Antonio María, López Lázaro, Miguel, Prieto Dapena, Francisco, González Rodríguez, María Luisa
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/128678
Online Access:https://hdl.handle.net/11441/128678
https://doi.org/10.3390/pharmaceutics13070973
Access Level:Open access
Keyword:Anchoring
Anticancer activity
Controlled drug release
Gold nanoparticles
Langmuir monolayers
Liposomal formulations
Temperature-sensitive nanocarriers
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spelling Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin deliveryGarcía, Mónica C.Naitlho, NabilaCalderón Montaño, José ManuelDrago, EstrellaRueda Rueda, ManuelaLonghi, MarcelaRabasco Álvarez, Antonio MaríaLópez Lázaro, MiguelPrieto Dapena, FranciscoGonzález Rodríguez, María LuisaAnchoringAnticancer activityControlled drug releaseGold nanoparticlesLangmuir monolayersLiposomal formulationsTemperature-sensitive nanocarriersStimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D) delivery. Their interfacial and morphological properties, drug release behavior against temperature changes and cytotoxic activity against breast and ovarian cancer cells were studied. Langmuir isotherms were performed to identify the most stable combination of lipid components. Two mole fractions of cholesterol (3.35 mol% and 40 mol%, L1 and L2 series, respectively) were evaluated. Thin-film hydration and transmembrane pH-gradient methods were used for preparing the L and for D loading, respectively. The cationic surface of L allowed the anchoring of negatively charged AuNPs by electrostatic interactions, even inducing a shift in the zeta potential of the L2 series. L exhibited nanometric sizes and spherical shape. The higher the proportion of cholesterol, the higher the drug loading. D was released in a controlled manner by diffusion-controlled mechanisms, and the proportions of cholesterol and temperature of release media influenced its release profiles. D-encapsulated L preserved its antiproliferative activity against cancer cells. The developed liposomal formulations exhibit promising properties for cancer treatment and potential for hyperthermia therapy.Ministerio de Ciencia e Innovación CTQ2014- 57515-C2-1Multidisciplinary Digital Publishing Institute (MDPI)Farmacia y Tecnología FarmacéuticaFarmacologíaQuímica Física2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/128678https://doi.org/10.3390/pharmaceutics13070973reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPharmaceutics, 13 (7), 973.CTQ2014- 57515-C2-1https://doi.org/10.3390/pharmaceutics13070973info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1286782026-06-17T12:51:07Z
dc.title.none.fl_str_mv Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
title Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
spellingShingle Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
García, Mónica C.
Anchoring
Anticancer activity
Controlled drug release
Gold nanoparticles
Langmuir monolayers
Liposomal formulations
Temperature-sensitive nanocarriers
title_short Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
title_full Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
title_fullStr Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
title_full_unstemmed Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
title_sort Cholesterol levels affect the performance of aunps-decorated thermo-sensitive liposomes as nanocarriers for controlled doxorubicin delivery
dc.creator.none.fl_str_mv García, Mónica C.
Naitlho, Nabila
Calderón Montaño, José Manuel
Drago, Estrella
Rueda Rueda, Manuela
Longhi, Marcela
Rabasco Álvarez, Antonio María
López Lázaro, Miguel
Prieto Dapena, Francisco
González Rodríguez, María Luisa
author García, Mónica C.
author_facet García, Mónica C.
Naitlho, Nabila
Calderón Montaño, José Manuel
Drago, Estrella
Rueda Rueda, Manuela
Longhi, Marcela
Rabasco Álvarez, Antonio María
López Lázaro, Miguel
Prieto Dapena, Francisco
González Rodríguez, María Luisa
author_role author
author2 Naitlho, Nabila
Calderón Montaño, José Manuel
Drago, Estrella
Rueda Rueda, Manuela
Longhi, Marcela
Rabasco Álvarez, Antonio María
López Lázaro, Miguel
Prieto Dapena, Francisco
González Rodríguez, María Luisa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Farmacia y Tecnología Farmacéutica
Farmacología
Química Física
dc.subject.none.fl_str_mv Anchoring
Anticancer activity
Controlled drug release
Gold nanoparticles
Langmuir monolayers
Liposomal formulations
Temperature-sensitive nanocarriers
topic Anchoring
Anticancer activity
Controlled drug release
Gold nanoparticles
Langmuir monolayers
Liposomal formulations
Temperature-sensitive nanocarriers
description Stimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D) delivery. Their interfacial and morphological properties, drug release behavior against temperature changes and cytotoxic activity against breast and ovarian cancer cells were studied. Langmuir isotherms were performed to identify the most stable combination of lipid components. Two mole fractions of cholesterol (3.35 mol% and 40 mol%, L1 and L2 series, respectively) were evaluated. Thin-film hydration and transmembrane pH-gradient methods were used for preparing the L and for D loading, respectively. The cationic surface of L allowed the anchoring of negatively charged AuNPs by electrostatic interactions, even inducing a shift in the zeta potential of the L2 series. L exhibited nanometric sizes and spherical shape. The higher the proportion of cholesterol, the higher the drug loading. D was released in a controlled manner by diffusion-controlled mechanisms, and the proportions of cholesterol and temperature of release media influenced its release profiles. D-encapsulated L preserved its antiproliferative activity against cancer cells. The developed liposomal formulations exhibit promising properties for cancer treatment and potential for hyperthermia therapy.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/128678
https://doi.org/10.3390/pharmaceutics13070973
url https://hdl.handle.net/11441/128678
https://doi.org/10.3390/pharmaceutics13070973
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pharmaceutics, 13 (7), 973.
CTQ2014- 57515-C2-1
https://doi.org/10.3390/pharmaceutics13070973
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412399768928256
score 15,300719

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