Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming

17 páginas, 5 figuras, 1 tabla.

Detalhes bibliográficos
Autores: Soler Palacios, Blanca, Villares, Ricardo, Lucas, Pilar, Rodríguez-Frade, José Miguel, Cayuela, Ana, Piccirillo, Jonathan G., Lombardía, Manuel, Delgado Gestoso, David, Fernández-García, Miguel, Risco, Cristina, Barbas, Coral, Corrales, Fernando J., Sorzano, Carlos Óscar S., Martínez-Martín, Nuria, Conesa, José J., Iborra, Francisco J., Mellado, Mario
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/334997
Acesso em linha:http://hdl.handle.net/10261/334997
Access Level:Acceso aberto
Palavra-chave:Cryo-FIB/SEM
Growth hormone
Macrophages
Metabolism
Mitochondria
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spelling Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogrammingSoler Palacios, BlancaVillares, RicardoLucas, PilarRodríguez-Frade, José MiguelCayuela, AnaPiccirillo, Jonathan G.Lombardía, ManuelDelgado Gestoso, DavidFernández-García, MiguelRisco, CristinaBarbas, CoralCorrales, Fernando J.Sorzano, Carlos Óscar S.Martínez-Martín, NuriaConesa, José J.Iborra, Francisco J.Mellado, MarioCryo-FIB/SEMGrowth hormoneMacrophagesMetabolismMitochondria17 páginas, 5 figuras, 1 tabla.Introduction: Macrophages are a heterogeneous population of innate immune cells that support tissue homeostasis through their involvement in tissue development and repair, and pathogen defense. Emerging data reveal that metabolism may control macrophage polarization and function and, conversely, phenotypic polarization may drive metabolic reprogramming. Methods: Here we use biochemical analysis, correlative cryogenic fluorescence microscopy and cryo-focused ion-beam scanning electron microscopy. Results: We demonstrate that growth hormone (GH) reprograms inflammatory GM-CSF-primed monocyte-derived macrophages (GM-MØ) by functioning as a metabolic modulator. We found that exogenous treatment of GM-MØ with recombinant human GH reduced glycolysis and lactate production to levels similar to those found in anti-inflammatory M-MØ. Moreover, GH treatment of GM-MØ augmented mitochondrial volume and altered mitochondrial dynamics, including the remodeling of the inner membrane to increase the density of cristae. Conclusions: Our data demonstrate that GH likely serves a modulatory role in the metabolism of inflammatory macrophages and suggest that metabolic reprogramming of macrophages should be considered as a new target to intervene in inflammatory diseases.This work was supported in part by grants from the Spanish Ministry of Science and Innovation (PID2020-114980RB-I00) Agencia Estatal de Investigación/Fondo Europeo de Desarrollo Regional (AEI/FEDER), Unión Europea, and by a grant from the Regional Government of Madrid P2022/BMD-7274. AC was supported by an FPI fellowship from the Spanish Ministry of Science and Innovation (PRE2018-086112) through the Severo Ochoa excellence accreditation SEV-2017-0712-18-1.With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (SEV-2017-0712-18-1)Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionComunidad de MadridIborra, Francisco J. [0000-0002-0692-3696]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/334997reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114980RB-I00P2022/BMD-7274/RETAR-A-COVID-CMinfo:eu-repo/grantAgreement/AEI//SEV-2017-0712-18-1https://dx.doi.org/10.3389/fimmu.2023.1200259Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3349972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
title Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
spellingShingle Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
Soler Palacios, Blanca
Cryo-FIB/SEM
Growth hormone
Macrophages
Metabolism
Mitochondria
title_short Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
title_full Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
title_fullStr Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
title_full_unstemmed Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
title_sort Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
dc.creator.none.fl_str_mv Soler Palacios, Blanca
Villares, Ricardo
Lucas, Pilar
Rodríguez-Frade, José Miguel
Cayuela, Ana
Piccirillo, Jonathan G.
Lombardía, Manuel
Delgado Gestoso, David
Fernández-García, Miguel
Risco, Cristina
Barbas, Coral
Corrales, Fernando J.
Sorzano, Carlos Óscar S.
Martínez-Martín, Nuria
Conesa, José J.
Iborra, Francisco J.
Mellado, Mario
author Soler Palacios, Blanca
author_facet Soler Palacios, Blanca
Villares, Ricardo
Lucas, Pilar
Rodríguez-Frade, José Miguel
Cayuela, Ana
Piccirillo, Jonathan G.
Lombardía, Manuel
Delgado Gestoso, David
Fernández-García, Miguel
Risco, Cristina
Barbas, Coral
Corrales, Fernando J.
Sorzano, Carlos Óscar S.
Martínez-Martín, Nuria
Conesa, José J.
Iborra, Francisco J.
Mellado, Mario
author_role author
author2 Villares, Ricardo
Lucas, Pilar
Rodríguez-Frade, José Miguel
Cayuela, Ana
Piccirillo, Jonathan G.
Lombardía, Manuel
Delgado Gestoso, David
Fernández-García, Miguel
Risco, Cristina
Barbas, Coral
Corrales, Fernando J.
Sorzano, Carlos Óscar S.
Martínez-Martín, Nuria
Conesa, José J.
Iborra, Francisco J.
Mellado, Mario
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Comunidad de Madrid
Iborra, Francisco J. [0000-0002-0692-3696]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cryo-FIB/SEM
Growth hormone
Macrophages
Metabolism
Mitochondria
topic Cryo-FIB/SEM
Growth hormone
Macrophages
Metabolism
Mitochondria
description 17 páginas, 5 figuras, 1 tabla.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/334997
url http://hdl.handle.net/10261/334997
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114980RB-I00
P2022/BMD-7274/RETAR-A-COVID-CM
info:eu-repo/grantAgreement/AEI//SEV-2017-0712-18-1
https://dx.doi.org/10.3389/fimmu.2023.1200259
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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