Xylitol production by Debaryomyces hansenii and Candida guilliermondii from rapeseed straw hemicellulosic hydrolysate

This study evaluated the possibility of using rapeseed straw hemicellulosic hydrolysate as a fermentation medium for xylitol production. Two yeast strains, namely Debaryomyces hansenii and Candida guilliermondii, were used for this bioconversion process and their performance to convert xylose into x...

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Detalhes bibliográficos
Autores: López-Linares, Juan Carlos, Romero, Inmaculada, Cara, Cristóbal, Castro, Eulogio, Mussatto, Solange
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Universidad de Jaén
Repositorio:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/2118
Acesso em linha:https://hdl.handle.net/10953/2118
Access Level:acceso abierto
Palavra-chave:Rapeseed straw
Hemicellulosic hydrolysate
Detoxification
Semi-defined media
Xylitol
Descrição
Resumo:This study evaluated the possibility of using rapeseed straw hemicellulosic hydrolysate as a fermentation medium for xylitol production. Two yeast strains, namely Debaryomyces hansenii and Candida guilliermondii, were used for this bioconversion process and their performance to convert xylose into xylitol was compared. Additionally, different strategies were evaluated for the hydrolysate detoxification before its use as a fermentation medium. Assays in semi-defined media containing different combinations of sugars were also performed in order to verify the influence of hexose sugars on xylose metabolism by the yeasts. C. guilliermondii exhibited higher tolerance to the toxic compounds than D. hansenii. Not only the toxic compounds present in the hydrolysate affected the yeast´s performance to convert xylose into xylitol, but glucose also had a negative impact on the bioconversion process. It was not necessary to completely eliminate the toxic compounds to obtain an efficient conversion of xylose into xylitol, mainly by C. guilliermondii.