Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract
Microglia, the resident macrophage-like population in the CNS, plays an important role in the pathogenesis of many neurodegenerative disorders. Nectandra genus is known to produce different metabolites with anti-inflammatory, anti-oxidant and analgesic properties. Although the species Nectandra angu...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:dnet:digitalcsic_::0bd067701806c8137ca889849dcb36b2 |
| Acceso en línea: | http://hdl.handle.net/10261/337133 |
| Access Level: | acceso abierto |
| Palabra clave: | Microglia Neuroinflammation Nectandra angustifolia extract Migration Phagocytosis ROS |
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Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| title |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| spellingShingle |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract Crescitelli, María Carla Microglia Neuroinflammation Nectandra angustifolia extract Migration Phagocytosis ROS |
| title_short |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| title_full |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| title_fullStr |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| title_full_unstemmed |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| title_sort |
Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract |
| dc.creator.none.fl_str_mv |
Crescitelli, María Carla Simón, Inmaculada Ferrini, Leandro Calvo, Hugo Torres, Ana M. Cabero, M. I. Macías Panedas, Mónica Rauschemberger, Maria B. Aguirre, María Victoria Rodríguez, Juan Pablo Hernández, Marita Nieto, María Luisa |
| author |
Crescitelli, María Carla |
| author_facet |
Crescitelli, María Carla Simón, Inmaculada Ferrini, Leandro Calvo, Hugo Torres, Ana M. Cabero, M. I. Macías Panedas, Mónica Rauschemberger, Maria B. Aguirre, María Victoria Rodríguez, Juan Pablo Hernández, Marita Nieto, María Luisa |
| author_role |
author |
| author2 |
Simón, Inmaculada Ferrini, Leandro Calvo, Hugo Torres, Ana M. Cabero, M. I. Macías Panedas, Mónica Rauschemberger, Maria B. Aguirre, María Victoria Rodríguez, Juan Pablo Hernández, Marita Nieto, María Luisa |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Agencia Nacional de Promoción Científica y Tecnológica (Argentina) Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) Fondo para la Investigación Científica y Tecnológica (Argentina) Universidad Nacional del Sur CSIC-UVA - Instituto de Biología y Genética Molecular (IBGM) Junta de Castilla y León Universidad de Valladolid Banco Santander Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Microglia Neuroinflammation Nectandra angustifolia extract Migration Phagocytosis ROS |
| topic |
Microglia Neuroinflammation Nectandra angustifolia extract Migration Phagocytosis ROS |
| description |
Microglia, the resident macrophage-like population in the CNS, plays an important role in the pathogenesis of many neurodegenerative disorders. Nectandra genus is known to produce different metabolites with anti-inflammatory, anti-oxidant and analgesic properties. Although the species Nectandra angustifolia is popularly used for the treatment of different types of inflammatory processes, its biological effects on neuroinflammation have not yet been addressed. In this study, we have investigated the role of a Nectandra angustifolia ethanolic extract (NaE) in lipopolysaccharide (LPS)-induced neuroinflammation in vitro and in vivo. In LPS-activated BV2 microglial cells, NaE significantly reduced the induced proinflammatory mediators TNF-α, IL-1β, IL-6, COX-2 and iNOS, as well as NO accumulation, while it promoted IL-10 secretion and YM-1 expression. Likewise, reduced CD14 expression levels were detected in microglial cells in the NaE+LPS group. NaE also attenuated LPS-induced ROS and lipid peroxidation build-up in BV2 cells. Mechanistically, NaE prevented NF-κB and MAPKs phosphorylation, as well as NLRP3 upregulation when added before LPS stimulation, although it did not affect the level of some proteins related to antioxidant defense such as Keap-1 and HO-1. Additionally, we observed that NaE modulated some activated microglia functions, decreasing cell migration, without affecting their phagocytic capabilities. In LPS-injected mice, NaE pre-treatment markedly suppressed the up-regulated TNF-α, IL-6 and IL-1β mRNA expression induced by LPS in brain. Our findings indicate that NaE is beneficial in preventing the neuroinflammatory response both in vivo and in vitro. NaE may regulate microglia homeostasis, not only restraining activation of LPS towards the M1 phenotype but promoting an M2 phenotype. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023 2023 2023 |
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info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/337133 |
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http://hdl.handle.net/10261/337133 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Multidisciplinary Digital Publishing Institute |
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Multidisciplinary Digital Publishing Institute |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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1869412357188354048 |
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Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic ExtractCrescitelli, María CarlaSimón, InmaculadaFerrini, LeandroCalvo, HugoTorres, Ana M.Cabero, M. I.Macías Panedas, MónicaRauschemberger, Maria B.Aguirre, María VictoriaRodríguez, Juan PabloHernández, MaritaNieto, María LuisaMicrogliaNeuroinflammationNectandra angustifolia extractMigrationPhagocytosisROSMicroglia, the resident macrophage-like population in the CNS, plays an important role in the pathogenesis of many neurodegenerative disorders. Nectandra genus is known to produce different metabolites with anti-inflammatory, anti-oxidant and analgesic properties. Although the species Nectandra angustifolia is popularly used for the treatment of different types of inflammatory processes, its biological effects on neuroinflammation have not yet been addressed. In this study, we have investigated the role of a Nectandra angustifolia ethanolic extract (NaE) in lipopolysaccharide (LPS)-induced neuroinflammation in vitro and in vivo. In LPS-activated BV2 microglial cells, NaE significantly reduced the induced proinflammatory mediators TNF-α, IL-1β, IL-6, COX-2 and iNOS, as well as NO accumulation, while it promoted IL-10 secretion and YM-1 expression. Likewise, reduced CD14 expression levels were detected in microglial cells in the NaE+LPS group. NaE also attenuated LPS-induced ROS and lipid peroxidation build-up in BV2 cells. Mechanistically, NaE prevented NF-κB and MAPKs phosphorylation, as well as NLRP3 upregulation when added before LPS stimulation, although it did not affect the level of some proteins related to antioxidant defense such as Keap-1 and HO-1. Additionally, we observed that NaE modulated some activated microglia functions, decreasing cell migration, without affecting their phagocytic capabilities. In LPS-injected mice, NaE pre-treatment markedly suppressed the up-regulated TNF-α, IL-6 and IL-1β mRNA expression induced by LPS in brain. Our findings indicate that NaE is beneficial in preventing the neuroinflammatory response both in vivo and in vitro. NaE may regulate microglia homeostasis, not only restraining activation of LPS towards the M1 phenotype but promoting an M2 phenotype.This work was supported by the Ministerio de Economía y Competitividad (RTC-2016-4852-2 and PID2019-111788RB-I00); Agencia Nacional de Promoción de Ciencia y Tecnología (ANPCYT) FONCYT-MinCyT, PICTO-UNNE 2019 00026, and CONICET, PUE—CONICET—229 20180100; Secretaría General de Ciencia y Tecnología (SGCyT)—Universidad Nacional del Sur, Bahía Blanca, Argentina (PGI 24/B328). M.L.N.’s institution is founded by the project “Programa Estratégico IBGM, grant CLU-2019-02-IBGM Unit of Excellence” from the Junta de Castilla y León (co-funded by FEDER, “Europe drives our growth”). I.S. was funded by the FPI Program from the Government of Castilla y León (co-funded by FSE). H.C. holds a predoctoral fellowship from Valladolid University-Banco de Santander. M.C.C. was funded by the program ERASMUS+ KA107.Multidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Agencia Nacional de Promoción Científica y Tecnológica (Argentina)Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Fondo para la Investigación Científica y Tecnológica (Argentina)Universidad Nacional del SurCSIC-UVA - Instituto de Biología y Genética Molecular (IBGM)Junta de Castilla y LeónUniversidad de ValladolidBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/337133reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//RTC-2016-4852-2info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111788RB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/antiox12020232http://dx.doi.org/10.3390/antiox12020232Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::0bd067701806c8137ca889849dcb36b22026-05-22T06:33:51Z |
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15,812429 |