Complexation of Hg with phytochelatins is important for plant Hg tolerance

Three-week-old alfalfa (Medicago sativa), barley (Hordeum vulgare) and maize (Zea mays) were exposed for 7 d to 30 µm of mercury (HgCl2) to characterize the Hg speciation in root, with no symptoms of being poisoned. The largest pool (99%) was associated with the particulate fraction, whereas the sol...

ver descrição completa

Detalhes bibliográficos
Autores: Carrasco Gil, Sandra, Álvarez Fernández, Ana, Sobrino Plata, Juan, Millán, Rocío, Carpena Ruiz, Ramón, Leduc, Danika, Andrews, Joy, Abadía, Javier, Hernández, Luís
Formato: artículo
Fecha de publicación:2011
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/99571
Acesso em linha:https://hdl.handle.net/20.500.14352/99571
Access Level:acceso abierto
Palavra-chave:581.4
Fisiología vegetal (Biología)
2417.19 Fisiología Vegetal
id ES_862ea9efde72849dbad8c964fcaddb6a
oai_identifier_str oai:docta.ucm.es:20.500.14352/99571
network_acronym_str ES
network_name_str España
repository_id_str
spelling Complexation of Hg with phytochelatins is important for plant Hg toleranceCarrasco Gil, SandraÁlvarez Fernández, AnaSobrino Plata, JuanMillán, RocíoCarpena Ruiz, RamónLeduc, DanikaAndrews, JoyAbadía, JavierHernández, Luís581.4Fisiología vegetal (Biología)2417.19 Fisiología VegetalThree-week-old alfalfa (Medicago sativa), barley (Hordeum vulgare) and maize (Zea mays) were exposed for 7 d to 30 µm of mercury (HgCl2) to characterize the Hg speciation in root, with no symptoms of being poisoned. The largest pool (99%) was associated with the particulate fraction, whereas the soluble fraction (SF) accounted for a minor proportion (<1%). Liquid chromatography coupled with electro-spray/time of flight mass spectrometry showed that Hg was bound to an array of phytochelatins (PCs) in root SF, which was particularly varied in alfalfa (eight ligands and five stoichiometries), a species that also accumulated homophytochelatins. Spatial localization of Hg in alfalfa roots by microprobe synchrotron X-ray fluorescence spectroscopy showed that most of the Hg co-localized with sulphur in the vascular cylinder. Extended X-ray Absorption Fine Structure (EXAFS) fingerprint fitting revealed that Hg was bound in vivo to organic-S compounds, i.e. biomolecules containing cysteine. Albeit a minor proportion of total Hg, Hg–PCs complexes in the SF might be important for tolerance to Hg, as was found with Arabidopsis thaliana mutants cad2-1 (with low glutathione content) and cad1-3 (unable to synthesize PCs) in comparison with wild type plants. Interestingly, high-performance liquid chromatography-electrospray ionization-time of flight analysis showed that none of these mutants accumulated Hg–biothiol complexes.WileyUniversidad Complutense de Madrid20112011-01-0120112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/99571reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/995712026-06-02T12:44:21Z
dc.title.none.fl_str_mv Complexation of Hg with phytochelatins is important for plant Hg tolerance
title Complexation of Hg with phytochelatins is important for plant Hg tolerance
spellingShingle Complexation of Hg with phytochelatins is important for plant Hg tolerance
Carrasco Gil, Sandra
581.4
Fisiología vegetal (Biología)
2417.19 Fisiología Vegetal
title_short Complexation of Hg with phytochelatins is important for plant Hg tolerance
title_full Complexation of Hg with phytochelatins is important for plant Hg tolerance
title_fullStr Complexation of Hg with phytochelatins is important for plant Hg tolerance
title_full_unstemmed Complexation of Hg with phytochelatins is important for plant Hg tolerance
title_sort Complexation of Hg with phytochelatins is important for plant Hg tolerance
dc.creator.none.fl_str_mv Carrasco Gil, Sandra
Álvarez Fernández, Ana
Sobrino Plata, Juan
Millán, Rocío
Carpena Ruiz, Ramón
Leduc, Danika
Andrews, Joy
Abadía, Javier
Hernández, Luís
author Carrasco Gil, Sandra
author_facet Carrasco Gil, Sandra
Álvarez Fernández, Ana
Sobrino Plata, Juan
Millán, Rocío
Carpena Ruiz, Ramón
Leduc, Danika
Andrews, Joy
Abadía, Javier
Hernández, Luís
author_role author
author2 Álvarez Fernández, Ana
Sobrino Plata, Juan
Millán, Rocío
Carpena Ruiz, Ramón
Leduc, Danika
Andrews, Joy
Abadía, Javier
Hernández, Luís
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 581.4
Fisiología vegetal (Biología)
2417.19 Fisiología Vegetal
topic 581.4
Fisiología vegetal (Biología)
2417.19 Fisiología Vegetal
description Three-week-old alfalfa (Medicago sativa), barley (Hordeum vulgare) and maize (Zea mays) were exposed for 7 d to 30 µm of mercury (HgCl2) to characterize the Hg speciation in root, with no symptoms of being poisoned. The largest pool (99%) was associated with the particulate fraction, whereas the soluble fraction (SF) accounted for a minor proportion (<1%). Liquid chromatography coupled with electro-spray/time of flight mass spectrometry showed that Hg was bound to an array of phytochelatins (PCs) in root SF, which was particularly varied in alfalfa (eight ligands and five stoichiometries), a species that also accumulated homophytochelatins. Spatial localization of Hg in alfalfa roots by microprobe synchrotron X-ray fluorescence spectroscopy showed that most of the Hg co-localized with sulphur in the vascular cylinder. Extended X-ray Absorption Fine Structure (EXAFS) fingerprint fitting revealed that Hg was bound in vivo to organic-S compounds, i.e. biomolecules containing cysteine. Albeit a minor proportion of total Hg, Hg–PCs complexes in the SF might be important for tolerance to Hg, as was found with Arabidopsis thaliana mutants cad2-1 (with low glutathione content) and cad1-3 (unable to synthesize PCs) in comparison with wild type plants. Interestingly, high-performance liquid chromatography-electrospray ionization-time of flight analysis showed that none of these mutants accumulated Hg–biothiol complexes.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01
2011
2011-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/99571
url https://hdl.handle.net/20.500.14352/99571
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412355261071361
score 15,301603

Registos relacionados