Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides

Bifidobacterium pseudocatenulatum CECT 7765 was isolated from stools of a breast-fed infant. Although, this strain is generally considered an adult-type bifidobacterial species, it has also been shown to have pre-clinical efficacy in obesity models. In order to understand the molecular basis of its...

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Detalles Bibliográficos
Autores: Benítez-Páez, Alfonso, Moreno, F. Javier, Sanz, M. Luz, Sanz Herranz, Yolanda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/150268
Acceso en línea:http://hdl.handle.net/10261/150268
Access Level:acceso abierto
Palabra clave:Lactulose-oligosaccharides
Probiotics
Gut microbiota
Leucine
Transcriptomes
Bifidobacterium pseudocatenulatum CECT7765
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spelling Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharidesBenítez-Páez, AlfonsoMoreno, F. JavierSanz, M. LuzSanz Herranz, YolandaLactulose-oligosaccharidesProbioticsGut microbiotaLeucineTranscriptomesBifidobacterium pseudocatenulatum CECT7765Bifidobacterium pseudocatenulatum CECT 7765 was isolated from stools of a breast-fed infant. Although, this strain is generally considered an adult-type bifidobacterial species, it has also been shown to have pre-clinical efficacy in obesity models. In order to understand the molecular basis of its adaptation to complex carbohydrates and improve its potential functionality, we have analyzed its genome and transcriptome, as well as its metabolic output when growing in galacto-oligosaccharides derived from lactulose (GOS-Lu) as carbon source. B. pseudocatenulatum CECT 7765 shows strain-specific genome regions, including a great diversity of sugar metabolic-related genes. A preliminary and exploratory transcriptome analysis suggests candidate over-expression of several genes coding for sugar transporters and permeases; furthermore, five out of seven beta-galactosidases identified in the genome could be activated in response to GOS-Lu exposure. Here, we also propose that a specific gene cluster is involved in controlling the import and hydrolysis of certain di- and tri-saccharides, which seemed to be those primarily taken-up by the bifidobacterial strain. This was discerned from mass spectrometry-based quantification of different saccharide fractions of culture supernatants. Our results confirm that the expression of genes involved in sugar transport and metabolism and in the synthesis of leucine, an amino acid with a key role in glucose and energy homeostasis, was up-regulated by GOS-Lu. This was done using qPCR in addition to the exploratory information derived from the single-replicated RNAseq approach, together with the functional annotation of genes predicted to be encoded in the B. pseudocatenulatum CETC 7765 genome.This study was supported by public grants AGL2011-27884 to FM and AGL2011-25169, and Consolider Fun-C-Food CSD2007-00063 to YS from the Spanish Ministry of Economy and Competitiveness (MINECO, Spain). The postdoctoral contract to AB-P was funded by AGL2011-25169 and the EU Project MyNewGut (no 613979) from the 7th Framework Program.Peer ReviewedFrontiers MediaMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/150268reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/613979https://doi.org/10.3389/fmicb.2016.00624Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1502682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
title Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
spellingShingle Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
Benítez-Páez, Alfonso
Lactulose-oligosaccharides
Probiotics
Gut microbiota
Leucine
Transcriptomes
Bifidobacterium pseudocatenulatum CECT7765
title_short Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
title_full Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
title_fullStr Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
title_full_unstemmed Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
title_sort Genome structure of the symbiont Bifidobacterium pseudocatenulatum CECT 7765 and gene expression profiling in response to lactulose-derived oligosaccharides
dc.creator.none.fl_str_mv Benítez-Páez, Alfonso
Moreno, F. Javier
Sanz, M. Luz
Sanz Herranz, Yolanda
author Benítez-Páez, Alfonso
author_facet Benítez-Páez, Alfonso
Moreno, F. Javier
Sanz, M. Luz
Sanz Herranz, Yolanda
author_role author
author2 Moreno, F. Javier
Sanz, M. Luz
Sanz Herranz, Yolanda
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lactulose-oligosaccharides
Probiotics
Gut microbiota
Leucine
Transcriptomes
Bifidobacterium pseudocatenulatum CECT7765
topic Lactulose-oligosaccharides
Probiotics
Gut microbiota
Leucine
Transcriptomes
Bifidobacterium pseudocatenulatum CECT7765
description Bifidobacterium pseudocatenulatum CECT 7765 was isolated from stools of a breast-fed infant. Although, this strain is generally considered an adult-type bifidobacterial species, it has also been shown to have pre-clinical efficacy in obesity models. In order to understand the molecular basis of its adaptation to complex carbohydrates and improve its potential functionality, we have analyzed its genome and transcriptome, as well as its metabolic output when growing in galacto-oligosaccharides derived from lactulose (GOS-Lu) as carbon source. B. pseudocatenulatum CECT 7765 shows strain-specific genome regions, including a great diversity of sugar metabolic-related genes. A preliminary and exploratory transcriptome analysis suggests candidate over-expression of several genes coding for sugar transporters and permeases; furthermore, five out of seven beta-galactosidases identified in the genome could be activated in response to GOS-Lu exposure. Here, we also propose that a specific gene cluster is involved in controlling the import and hydrolysis of certain di- and tri-saccharides, which seemed to be those primarily taken-up by the bifidobacterial strain. This was discerned from mass spectrometry-based quantification of different saccharide fractions of culture supernatants. Our results confirm that the expression of genes involved in sugar transport and metabolism and in the synthesis of leucine, an amino acid with a key role in glucose and energy homeostasis, was up-regulated by GOS-Lu. This was done using qPCR in addition to the exploratory information derived from the single-replicated RNAseq approach, together with the functional annotation of genes predicted to be encoded in the B. pseudocatenulatum CETC 7765 genome.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/150268
url http://hdl.handle.net/10261/150268
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/613979
https://doi.org/10.3389/fmicb.2016.00624
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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