An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar

The small arboreal frog Guibemantis liber (Anura: Mantellidae) has served as an example for the existence of deep conspecific lineages that differ by a substantial amount in mitochondrial DNA but are similar in morphology and bioacoustics and thus are assigned to the same nominal species. During fie...

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Autores: Koppetsch, Thore, Pabijan, Maciej, Hutter, Carl R., Köhler, Jörn, Gehring, Philip-Sebastian, Rakotoarison, Andolalao, Ratsoavina, Fanomezana M., Scherz, Mark D., David Vieites
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330218
Acceso en línea:http://hdl.handle.net/10261/330218
Access Level:acceso abierto
Palabra clave:Amphibia
Anura
Bioacoustics
FrogCap
Morphology
Pandanusicola
Phylogeography
Taxonomy
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spelling An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern MadagascarKoppetsch, ThorePabijan, MaciejHutter, Carl R.Köhler, JörnGehring, Philip-SebastianRakotoarison, AndolalaoRatsoavina, Fanomezana M.Scherz, Mark D.David VieitesAmphibiaAnuraBioacousticsFrogCapMorphologyPandanusicolaPhylogeographyTaxonomyThe small arboreal frog Guibemantis liber (Anura: Mantellidae) has served as an example for the existence of deep conspecific lineages that differ by a substantial amount in mitochondrial DNA but are similar in morphology and bioacoustics and thus are assigned to the same nominal species. During fieldwork in northern Madagascar, we identified additional such lineages and surprisingly, observed close syntopy of two of these at various sites. In-depth study based on DNA sequences of the mitochondrial cytochrome b gene from 338 specimens of G. liber sensu lato from across its range, sequences of four nuclear-encoded markers for 154‒257 of these specimens, a phylogenomic dataset obtained by the FrogCap target capture approach, and additional mitochondrial genes for representatives of most mitochondrial lineages, as well as bioacoustic and morphological comparisons, revealed concordant differentiation among several lineages of the G. liber complex. We identify nine lineages differing by 5.3‒15.5% in cytochrome b and 2.4‒10.1% in the 16S rRNA gene, and find that several of these lack or have only limited allele sharing in the nuclear-encoded genes. Based on sympatric or parapatric occurrence without genetic admixture, combined with differences in bioacoustic and morphological characters, we scientifically name three lineages from northern Madagascar as new species: G. razoky sp. nov., G. razandry sp. nov., and G. fotsitenda sp. nov. Of these new species, G. razoky sp. nov. and G. razandry sp. nov. show widespread syntopy across northern Madagascar and differ in body size and advertisement calls. Guibemantis fotsitenda sp. nov. is sister to G. razandry sp. nov., but appears to occur at lower elevations, including in close geographic proximity on the Marojejy Massif. We also detected subtle differences in advertisement calls among various other mitochondrial lineages distributed in the Northern Central East and Southern Central East of Madagascar, but the status and nomenclatural identity of these lineages require further morphological and bioacoustic study of reliably genotyped individuals, and assignment of the three available names in the complex: Rhacophorus liber Peracca, 1893, Gephyromantis albogularis Guibé, 1947, and Gephyromantis variabilis Millot and Guibé, 1951. We discuss the identity and type material of these three nomina, designate a lectotype for Gephyromantis variabilis from Itremo, and flag the collection of new material from their type localities, Andrangoloaka and Itremo, as paramount for a comprehensive revision of the G. liber complex.Field expeditions were supported, among other funders, by the Volkswagen Foundation, Deutsche Forschungsgemeinschaft, and Deutscher Akademischer Austauschdienst. A research stay of TK at TU Braunschweig was supported by a grant of the Alexander-Koenig-Gesellschaft. MP was supported by the Alexander von Humboldt Foundation. Fieldwork for CRH was supported by Global Wildlife Conservation Grant 5019-0096.Peer reviewedPensoft PublishersVolkswagen FoundationGerman Research FoundationGerman Academic Exchange ServiceAlexander Koenig SocietyAlexander von Humboldt FoundationGlobal Wildlife ConservationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/330218reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3897/vz.73.e94063Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3302182026-05-22T06:33:51Z
dc.title.none.fl_str_mv An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
title An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
spellingShingle An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
Koppetsch, Thore
Amphibia
Anura
Bioacoustics
FrogCap
Morphology
Pandanusicola
Phylogeography
Taxonomy
title_short An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
title_full An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
title_fullStr An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
title_full_unstemmed An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
title_sort An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
dc.creator.none.fl_str_mv Koppetsch, Thore
Pabijan, Maciej
Hutter, Carl R.
Köhler, Jörn
Gehring, Philip-Sebastian
Rakotoarison, Andolalao
Ratsoavina, Fanomezana M.
Scherz, Mark D.
David Vieites
author Koppetsch, Thore
author_facet Koppetsch, Thore
Pabijan, Maciej
Hutter, Carl R.
Köhler, Jörn
Gehring, Philip-Sebastian
Rakotoarison, Andolalao
Ratsoavina, Fanomezana M.
Scherz, Mark D.
David Vieites
author_role author
author2 Pabijan, Maciej
Hutter, Carl R.
Köhler, Jörn
Gehring, Philip-Sebastian
Rakotoarison, Andolalao
Ratsoavina, Fanomezana M.
Scherz, Mark D.
David Vieites
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Volkswagen Foundation
German Research Foundation
German Academic Exchange Service
Alexander Koenig Society
Alexander von Humboldt Foundation
Global Wildlife Conservation
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Amphibia
Anura
Bioacoustics
FrogCap
Morphology
Pandanusicola
Phylogeography
Taxonomy
topic Amphibia
Anura
Bioacoustics
FrogCap
Morphology
Pandanusicola
Phylogeography
Taxonomy
description The small arboreal frog Guibemantis liber (Anura: Mantellidae) has served as an example for the existence of deep conspecific lineages that differ by a substantial amount in mitochondrial DNA but are similar in morphology and bioacoustics and thus are assigned to the same nominal species. During fieldwork in northern Madagascar, we identified additional such lineages and surprisingly, observed close syntopy of two of these at various sites. In-depth study based on DNA sequences of the mitochondrial cytochrome b gene from 338 specimens of G. liber sensu lato from across its range, sequences of four nuclear-encoded markers for 154‒257 of these specimens, a phylogenomic dataset obtained by the FrogCap target capture approach, and additional mitochondrial genes for representatives of most mitochondrial lineages, as well as bioacoustic and morphological comparisons, revealed concordant differentiation among several lineages of the G. liber complex. We identify nine lineages differing by 5.3‒15.5% in cytochrome b and 2.4‒10.1% in the 16S rRNA gene, and find that several of these lack or have only limited allele sharing in the nuclear-encoded genes. Based on sympatric or parapatric occurrence without genetic admixture, combined with differences in bioacoustic and morphological characters, we scientifically name three lineages from northern Madagascar as new species: G. razoky sp. nov., G. razandry sp. nov., and G. fotsitenda sp. nov. Of these new species, G. razoky sp. nov. and G. razandry sp. nov. show widespread syntopy across northern Madagascar and differ in body size and advertisement calls. Guibemantis fotsitenda sp. nov. is sister to G. razandry sp. nov., but appears to occur at lower elevations, including in close geographic proximity on the Marojejy Massif. We also detected subtle differences in advertisement calls among various other mitochondrial lineages distributed in the Northern Central East and Southern Central East of Madagascar, but the status and nomenclatural identity of these lineages require further morphological and bioacoustic study of reliably genotyped individuals, and assignment of the three available names in the complex: Rhacophorus liber Peracca, 1893, Gephyromantis albogularis Guibé, 1947, and Gephyromantis variabilis Millot and Guibé, 1951. We discuss the identity and type material of these three nomina, designate a lectotype for Gephyromantis variabilis from Itremo, and flag the collection of new material from their type localities, Andrangoloaka and Itremo, as paramount for a comprehensive revision of the G. liber complex.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330218
url http://hdl.handle.net/10261/330218
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3897/vz.73.e94063
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Pensoft Publishers
publisher.none.fl_str_mv Pensoft Publishers
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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