Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis

Background: Growing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low- density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a coho...

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Autores: Fernandez-Bravo, S, Canyelles, M, Martín-Blázquez, A, Borràs, C, Nuñez-Borque, E, Palacio-García, L, Del Rio, PR, Betancor, D, Gómez-López, A, Brescó, MS, Laguna, JJ, Méndez-Barbero, N, Rotllan, N, Escolà-Gil, JC, Esteban, V
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p18650
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18650
http://ddd.uab.cat/record/309009
Access Level:acceso abierto
Palabra clave:Anaphylaxis
high-density lipoprotein cholesterol
macrophage cholesterol efflux
vascular permeability
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spelling Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxisFernandez-Bravo, SCanyelles, MMartín-Blázquez, ABorràs, CNuñez-Borque, EPalacio-García, LDel Rio, PRBetancor, DGómez-López, ABrescó, MSLaguna, JJMéndez-Barbero, NRotllan, NEscolà-Gil, JCEsteban, VAnaphylaxishigh-density lipoprotein cholesterolmacrophage cholesterol effluxvascular permeabilityBackground: Growing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low- density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a cohort of patients presenting with hypersensitivity reactions. We further assessed the function of high-density lipoprotein particles as well as their involvement in the molecular mechanisms of anaphylaxis. Methods: Lipid profile determination was performed in paired (acute and baseline) serum samples from 153 patients. Thirtyeight experienced a non-anaphylactic reaction and 115 had an anaphylactic reaction (88 moderate and 27 severe). Lecithin cholesterol acyl transferase activity was assessed in patient sera, and we also evaluated macrophage cholesterol efflux in response to the serum samples. Last, the effect of anaphylactic-derived high-density lipoprotein (HDL) particles on the endothelial barrier was studied. Detailed methods are provided in the Methods section in this article's Online Repository available at www.jacionline.org. Results: Serum samples from severe anaphylactic reactions show statistically significant low levels of HDL cholesterol, low-density lipoprotein cholesterol, and apolipoproteins A1 and B, which points to their possible role as biomarkers. Specifically, HDL particles play a protective role in cardiovascular diseases. Using functional human serum cell assays, we observed impaired capacity of apolipoprotein B- depleted serum to induce macrophage cholesterol efflux in severe anaphylactic reactions. In addition, purified HDL particles from human anaphylactic sera failed to stabilize and maintain the endothelial barrier. Conclusion: These results encourage further research on HDL functions in severe anaphylaxis, which may lead to new diagnostic and therapeutic strategies.MOSBY-ELSEVIER2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18650http://ddd.uab.cat/record/309009JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGYISSN: 00916749ISSNe: 10976825reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p186502026-06-14T12:41:47Z
dc.title.none.fl_str_mv Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
spellingShingle Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
Fernandez-Bravo, S
Anaphylaxis
high-density lipoprotein cholesterol
macrophage cholesterol efflux
vascular permeability
title_short Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_full Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_fullStr Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_full_unstemmed Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_sort Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
dc.creator.none.fl_str_mv Fernandez-Bravo, S
Canyelles, M
Martín-Blázquez, A
Borràs, C
Nuñez-Borque, E
Palacio-García, L
Del Rio, PR
Betancor, D
Gómez-López, A
Brescó, MS
Laguna, JJ
Méndez-Barbero, N
Rotllan, N
Escolà-Gil, JC
Esteban, V
author Fernandez-Bravo, S
author_facet Fernandez-Bravo, S
Canyelles, M
Martín-Blázquez, A
Borràs, C
Nuñez-Borque, E
Palacio-García, L
Del Rio, PR
Betancor, D
Gómez-López, A
Brescó, MS
Laguna, JJ
Méndez-Barbero, N
Rotllan, N
Escolà-Gil, JC
Esteban, V
author_role author
author2 Canyelles, M
Martín-Blázquez, A
Borràs, C
Nuñez-Borque, E
Palacio-García, L
Del Rio, PR
Betancor, D
Gómez-López, A
Brescó, MS
Laguna, JJ
Méndez-Barbero, N
Rotllan, N
Escolà-Gil, JC
Esteban, V
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anaphylaxis
high-density lipoprotein cholesterol
macrophage cholesterol efflux
vascular permeability
topic Anaphylaxis
high-density lipoprotein cholesterol
macrophage cholesterol efflux
vascular permeability
description Background: Growing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low- density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a cohort of patients presenting with hypersensitivity reactions. We further assessed the function of high-density lipoprotein particles as well as their involvement in the molecular mechanisms of anaphylaxis. Methods: Lipid profile determination was performed in paired (acute and baseline) serum samples from 153 patients. Thirtyeight experienced a non-anaphylactic reaction and 115 had an anaphylactic reaction (88 moderate and 27 severe). Lecithin cholesterol acyl transferase activity was assessed in patient sera, and we also evaluated macrophage cholesterol efflux in response to the serum samples. Last, the effect of anaphylactic-derived high-density lipoprotein (HDL) particles on the endothelial barrier was studied. Detailed methods are provided in the Methods section in this article's Online Repository available at www.jacionline.org. Results: Serum samples from severe anaphylactic reactions show statistically significant low levels of HDL cholesterol, low-density lipoprotein cholesterol, and apolipoproteins A1 and B, which points to their possible role as biomarkers. Specifically, HDL particles play a protective role in cardiovascular diseases. Using functional human serum cell assays, we observed impaired capacity of apolipoprotein B- depleted serum to induce macrophage cholesterol efflux in severe anaphylactic reactions. In addition, purified HDL particles from human anaphylactic sera failed to stabilize and maintain the endothelial barrier. Conclusion: These results encourage further research on HDL functions in severe anaphylaxis, which may lead to new diagnostic and therapeutic strategies.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18650
http://ddd.uab.cat/record/309009
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18650
http://ddd.uab.cat/record/309009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MOSBY-ELSEVIER
publisher.none.fl_str_mv MOSBY-ELSEVIER
dc.source.none.fl_str_mv JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN: 00916749
ISSNe: 10976825
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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