Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)

14 pages, 7 figures, 3 tables

Detalles Bibliográficos
Autores: Tarbe, Anne-Laure, Stenuite, Stephane, Balagué, Vanessa, Sinyinza, Danny, Descy, Jean-Pierre, Massana, Ramon
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/46832
Acceso en línea:http://hdl.handle.net/10261/46832
Access Level:acceso abierto
Palabra clave:Small-eukaryote community
Tropical lake
18S rRNA
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spelling Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)Tarbe, Anne-LaureStenuite, StephaneBalagué, VanessaSinyinza, DannyDescy, Jean-PierreMassana, RamonSmall-eukaryote communityTropical lake18S rRNA14 pages, 7 figures, 3 tablesIn aquatic environments, small eukaryotes (mainly algae and protozoa of 1 to 5 µm in size) are a key link in the carbon transfer to higher trophic levels, e.g. through primary production and grazing of picoplankton. However, the diversity of these microorganisms remains poorly investigated in freshwater habitats, and is still unknown in tropical aquatic systems. In this study, we investigated the small-eukaryote diversity in the oligotrophic Lake Tanganyika, one of the African Great Lakes, at different depths in the water column using denaturing gradient gel electrophoresis (DGGE) and gene clone libraries based on 18S rRNA genes. Each sample produced complex DGGE fingerprints clearly discriminating the epilimnion from the metalimnion. Analysis, using genetic libraries, confirmed the high level of small-eukaryote diversity in Lake Tanganyika. Organisms from 5 taxonomic groups (Stramenopiles, Alveolata, Cryptophyta, Kinetoplastea and Choanoflagellida) were dominant among the species detected. Some sequences were nearly identical to those recovered in temperate freshwaters in North America and Europe, suggesting a high dispersal ability in some small-eukaryote lineages. However, 49% of sequences were <95% similar to any sequence in GenBank. This may result from undersampling of freshwater systems, but also raises the possibility that perennially warm tropical waters harbour particular assemblages of planktonic small eukaryotesThis study was supported by the FNRS (National Fund for Scientific Research) through the PhD scholarship to A.L.T. and to project GEMMA (CTM2007-63753-C02-01/MAR, MEC) to R.M.Peer reviewedInter Research201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/46832reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3354/ame01465info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/468322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
title Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
spellingShingle Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
Tarbe, Anne-Laure
Small-eukaryote community
Tropical lake
18S rRNA
title_short Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
title_full Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
title_fullStr Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
title_full_unstemmed Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
title_sort Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)
dc.creator.none.fl_str_mv Tarbe, Anne-Laure
Stenuite, Stephane
Balagué, Vanessa
Sinyinza, Danny
Descy, Jean-Pierre
Massana, Ramon
author Tarbe, Anne-Laure
author_facet Tarbe, Anne-Laure
Stenuite, Stephane
Balagué, Vanessa
Sinyinza, Danny
Descy, Jean-Pierre
Massana, Ramon
author_role author
author2 Stenuite, Stephane
Balagué, Vanessa
Sinyinza, Danny
Descy, Jean-Pierre
Massana, Ramon
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Small-eukaryote community
Tropical lake
18S rRNA
topic Small-eukaryote community
Tropical lake
18S rRNA
description 14 pages, 7 figures, 3 tables
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/46832
url http://hdl.handle.net/10261/46832
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3354/ame01465
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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