Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.

Aminoglycoside resistance was evaluated in 690 enterococcus strains isolated from different clinical sources originating from patients at the University Clinic Hospital of Zaragoza (Spain). The enterococci obtained from clinically significant samples (blood, urine, or exudates) showed more high- lev...

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Authors: Del Campo, R. [0000-0003-1147-7923], Tenorio, C. [0000-0002-8099-1208], Rubio, C., Castillo, J. [0000-0002-2519-701X], Torres, C. [0000-0003-3709-1690], Gómez-Lus, R.
Format: article
Status:Published version
Publication Date:2000
Country:España
Institution:Universidad de La Rioja (UR)
Repository:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc6905b750603269e81394
Online Access:https://investigacion.unirioja.es/documentos/5bbc6905b750603269e81394
Access Level:Open access
Keyword:Aminoglycoside-modifying-enzymes
Enterococcus
Gentamicin
Streptomycin
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spelling Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.Del Campo, R. [0000-0003-1147-7923]Tenorio, C. [0000-0002-8099-1208]Rubio, C.Castillo, J. [0000-0002-2519-701X]Torres, C. [0000-0003-3709-1690]Gómez-Lus, R.Aminoglycoside-modifying-enzymesEnterococcusGentamicinStreptomycinAminoglycoside resistance was evaluated in 690 enterococcus strains isolated from different clinical sources originating from patients at the University Clinic Hospital of Zaragoza (Spain). The enterococci obtained from clinically significant samples (blood, urine, or exudates) showed more high- level resistance to gentamicin and streptomycin (65 and 42%, respectively) than those isolated from faecal samples (49 and 23%, respectively). Aminoglycoside-modifying enzymes (AME) from 119 of these high-level gentamicin and streptomycin resistant enterococcus strains were studied. The most frequent AMEs found were APH(3') and AAC(6')-APH(2''). More than one enzyme was detected in 71% of the strains (four different enzymes in 5% of the strains). Three Enterococcus faecalis strains had ANT(4')(4'') enzymatic activity. Different enzymatic expressions of the bifunctional enzyme AAC(6')- APH(2'') were demonstrated in strains in which the complete aac(6')-aph(2'') gene was detected by PCR and hybridization: (i) AAC(6') + APH(2'') activity; (ii) AAC(6') only; (iii) APH(2'') only; and (iv) no activity of AAC(6') or APH(2''). (C) 2000 Elsevier Science B.V. and International Society of Chemotherapy.2000info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc6905b750603269e81394reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0924-8579(00)00169-2info:eu-repo/semantics/altIdentifier/wos/WOS:000088206800009info:eu-repo/semantics/altIdentifier/pmid/10926445info:eu-repo/semantics/altIdentifier/pissn/0924-8579Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain., 2000, vol. 15, núm. 3, pág. 221-226info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc6905b750603269e813942026-06-14T12:47:17Z
dc.title.none.fl_str_mv Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
title Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
spellingShingle Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
Del Campo, R. [0000-0003-1147-7923]
Aminoglycoside-modifying-enzymes
Enterococcus
Gentamicin
Streptomycin
title_short Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
title_full Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
title_fullStr Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
title_full_unstemmed Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
title_sort Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain.
dc.creator.none.fl_str_mv Del Campo, R. [0000-0003-1147-7923]
Tenorio, C. [0000-0002-8099-1208]
Rubio, C.
Castillo, J. [0000-0002-2519-701X]
Torres, C. [0000-0003-3709-1690]
Gómez-Lus, R.
author Del Campo, R. [0000-0003-1147-7923]
author_facet Del Campo, R. [0000-0003-1147-7923]
Tenorio, C. [0000-0002-8099-1208]
Rubio, C.
Castillo, J. [0000-0002-2519-701X]
Torres, C. [0000-0003-3709-1690]
Gómez-Lus, R.
author_role author
author2 Tenorio, C. [0000-0002-8099-1208]
Rubio, C.
Castillo, J. [0000-0002-2519-701X]
Torres, C. [0000-0003-3709-1690]
Gómez-Lus, R.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Aminoglycoside-modifying-enzymes
Enterococcus
Gentamicin
Streptomycin
topic Aminoglycoside-modifying-enzymes
Enterococcus
Gentamicin
Streptomycin
description Aminoglycoside resistance was evaluated in 690 enterococcus strains isolated from different clinical sources originating from patients at the University Clinic Hospital of Zaragoza (Spain). The enterococci obtained from clinically significant samples (blood, urine, or exudates) showed more high- level resistance to gentamicin and streptomycin (65 and 42%, respectively) than those isolated from faecal samples (49 and 23%, respectively). Aminoglycoside-modifying enzymes (AME) from 119 of these high-level gentamicin and streptomycin resistant enterococcus strains were studied. The most frequent AMEs found were APH(3') and AAC(6')-APH(2''). More than one enzyme was detected in 71% of the strains (four different enzymes in 5% of the strains). Three Enterococcus faecalis strains had ANT(4')(4'') enzymatic activity. Different enzymatic expressions of the bifunctional enzyme AAC(6')- APH(2'') were demonstrated in strains in which the complete aac(6')-aph(2'') gene was detected by PCR and hybridization: (i) AAC(6') + APH(2'') activity; (ii) AAC(6') only; (iii) APH(2'') only; and (iv) no activity of AAC(6') or APH(2''). (C) 2000 Elsevier Science B.V. and International Society of Chemotherapy.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc6905b750603269e81394
url https://investigacion.unirioja.es/documentos/5bbc6905b750603269e81394
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0924-8579(00)00169-2
info:eu-repo/semantics/altIdentifier/wos/WOS:000088206800009
info:eu-repo/semantics/altIdentifier/pmid/10926445
info:eu-repo/semantics/altIdentifier/pissn/0924-8579
Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp strains in Spain., 2000, vol. 15, núm. 3, pág. 221-226
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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