Biofabrication of functional protein nanoparticles through simple His-tag engineering

We have developed a simple, robust, and fully transversal approach for thea-la-cartefabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination b...

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Bibliographic Details
Authors: López-Laguna, Hèctor|||0000-0001-5249-8304, Sánchez, Julieta M.|||0000-0001-6676-5776, Carratalá, José Vicente|||0000-0001-6950-2939, Rojas Peña, Mauricio|||0000-0002-2373-1792, Sánchez-García, Laura|||0000-0002-8420-1701, Parladé Molist, Eloi|||0000-0001-5750-550X, Sánchez Chardi, Alejandro|||0000-0002-8789-1883, Voltà-Durán, Eric|||0000-0003-0017-8274, Serna, Naroa|||0000-0001-5682-8198, Cano-Garrido, Olivia|||0000-0002-5504-2131, Flores, Sandra, Ferrer-Miralles, Neus|||0000-0003-2981-3913, Nolan, Verónica, de Marco, Ario|||0000-0001-7729-819X, Roher Armentia, Nerea|||0000-0002-6659-4038, Unzueta Elorza, Ugutz|||0000-0001-5119-2266, Vázquez, Esther|||0000-0003-1052-0424, Villaverde, Antonio|||0000-0002-2615-4521
Format: article
Publication Date:2021
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:250460
Online Access:https://ddd.uab.cat/record/250460
https://dx.doi.org/urn:doi:10.1021/acssuschemeng.1c04256
Access Level:Open access
Keyword:Nanoparticles
Protein engineering
Divalent cations
Protein materials
Biomaterials design
Description
Summary:We have developed a simple, robust, and fully transversal approach for thea-la-cartefabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Znions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.

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